##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063028_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1130140
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234572707806112	32.0	32.0	32.0	32.0	32.0
2	30.934830198028564	32.0	32.0	32.0	32.0	32.0
3	30.988411170297486	32.0	32.0	32.0	32.0	32.0
4	31.014033659546605	32.0	32.0	32.0	32.0	32.0
5	30.994715698939956	32.0	32.0	32.0	32.0	32.0
6	34.59250712301131	36.0	36.0	36.0	32.0	36.0
7	34.526542729219386	36.0	36.0	36.0	32.0	36.0
8	34.51788893411436	36.0	36.0	36.0	32.0	36.0
9	34.61053320827508	36.0	36.0	36.0	32.0	36.0
10	34.37626400268993	36.0	36.0	36.0	32.0	36.0
11	34.60997929460067	36.0	36.0	36.0	32.0	36.0
12	34.45664165501619	36.0	36.0	36.0	32.0	36.0
13	34.53738386394606	36.0	36.0	36.0	32.0	36.0
14	34.424154529527314	36.0	36.0	36.0	32.0	36.0
15	34.40164138956236	36.0	36.0	36.0	32.0	36.0
16	34.410941122338826	36.0	36.0	36.0	32.0	36.0
17	34.33129435291203	36.0	36.0	36.0	32.0	36.0
18	34.361904719769235	36.0	36.0	36.0	32.0	36.0
19	34.3413647866636	36.0	36.0	36.0	32.0	36.0
20	34.31632275647265	36.0	36.0	36.0	32.0	36.0
21	34.28741483356045	36.0	36.0	36.0	32.0	36.0
22	34.27914506167377	36.0	36.0	36.0	32.0	36.0
23	34.232670288636804	36.0	36.0	36.0	32.0	36.0
24	34.22678694674996	36.0	36.0	36.0	32.0	36.0
25	34.210131488134216	36.0	36.0	36.0	32.0	36.0
26	34.16347797617994	36.0	36.0	36.0	32.0	36.0
27	34.16252145751854	36.0	36.0	36.0	32.0	36.0
28	34.1449377953174	36.0	36.0	36.0	32.0	36.0
29	34.12873183853328	36.0	36.0	36.0	32.0	36.0
30	34.09163201019343	36.0	36.0	36.0	32.0	36.0
31	34.11627939901251	36.0	36.0	36.0	32.0	36.0
32	34.06370626648026	36.0	36.0	36.0	32.0	36.0
33	34.01746863220486	36.0	36.0	36.0	32.0	36.0
34	34.01848797494116	36.0	36.0	36.0	32.0	36.0
35	33.9861415399862	36.0	36.0	36.0	32.0	36.0
36	33.94489001362663	36.0	36.0	36.0	32.0	36.0
37	33.95415877678872	36.0	36.0	36.0	32.0	36.0
38	33.29028792892916	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	3.0
9	4.0
10	8.0
11	20.0
12	5.0
13	3.0
14	176.0
15	333.0
16	428.0
17	600.0
18	715.0
19	941.0
20	1267.0
21	1708.0
22	2307.0
23	3317.0
24	4607.0
25	6799.0
26	9819.0
27	14085.0
28	20152.0
29	26837.0
30	37014.0
31	50123.0
32	69868.0
33	108305.0
34	248255.0
35	522435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86106209115586	17.14514277209619	11.367018901622265	25.626776235125693
2	17.13603510126318	19.24097767925123	36.839770807732755	26.783216411752832
3	19.19132476299113	23.11160589051	28.022665065516485	29.67440428098239
4	12.639820336232457	14.705281162646855	35.20583376323211	37.44906473788858
5	15.146645807857093	36.00445255358195	32.86855233608639	15.980349302474565
6	35.18187191630034	34.90553349331408	16.342546383798055	13.570048206587526
7	31.244768338025974	29.957668886454574	20.241244874519747	18.5563179009997
8	29.091974130478448	31.95128360998219	18.965201440892354	19.991540818647007
9	27.529237709189978	13.93092671393544	18.569318832385214	39.970516744489366
10	16.519692424765292	25.695008538840664	30.231831736171944	27.553467300222103
11	38.40144198862762	20.82680739682826	21.632572519745935	19.139178094798186
12	25.494313350199054	23.30460143898388	27.710925464316084	23.490159746500982
13	29.36600647799076	18.66962247119418	25.441954724862388	26.52241632595267
14	23.9871543688066	19.671708992826765	24.383160152703372	31.957976485663263
15	25.409245550870708	26.81275245954452	22.117314697791986	25.660687291792783
16	26.41196027989207	25.313383014441506	23.203484580184018	25.07117212548241
17	24.37391419015733	25.598074902846847	24.66508601959259	25.36292488740323
18	25.673362983309055	23.919053088558805	26.043161775246578	24.364422152885563
19	25.75938848939962	24.422549718862992	25.27958840904948	24.538473382687904
20	26.149231014282424	23.494770189193495	24.60869334371626	25.74730545280782
21	27.66975084685169	23.561502493655247	24.228453508992377	24.540293150500677
22	26.4401890165124	23.500079641788933	24.878767499070843	25.180963842627825
23	24.451891687160348	23.362887304063722	25.805403960804334	26.37981704797159
24	25.090046000895594	24.605877282566563	25.104825049691232	25.19925166684661
25	25.18273077974868	23.925673995367898	25.070070030205116	25.82152519467831
26	24.819612559727204	25.4042510635624	25.53753377424599	24.2386026024644
27	25.724684812878802	24.154656061760484	24.81533888533445	25.305320240026262
28	24.752204519209073	24.224093304367447	25.865060859331102	25.15864131709238
29	24.432531163220407	24.532352581994267	25.739589988832034	25.295526265953285
30	24.340123015988894	24.80495037151678	26.063514901818163	24.791411710676165
31	25.297172645515708	24.78631797937644	24.351211034389998	25.565298340717852
32	24.946971889128108	24.556019542307894	24.160731146371745	26.336277422192257
33	24.43090711512118	24.127733079419915	25.213000555730574	26.228359249728328
34	25.762606570230602	24.321640770049008	25.144910734987246	24.77084192473314
35	26.18487124254616	24.0182343706027	25.46439943572924	24.332494951121905
36	24.571469027801747	25.14538060913391	24.639435734735144	25.643714628329196
37	25.949887967377798	24.78204079898905	24.590275639187702	24.67779559444545
38	24.705593979852612	24.422957121316163	25.211136989501554	25.66031190932967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	115.5
2	160.0
3	160.0
4	433.0
5	706.0
6	706.0
7	907.0
8	1108.0
9	1117.0
10	1126.0
11	1126.0
12	1434.0
13	1742.0
14	2372.5
15	3003.0
16	3003.0
17	4372.0
18	5741.0
19	5741.0
20	6630.0
21	7519.0
22	7395.5
23	7272.0
24	7272.0
25	8328.5
26	9385.0
27	9385.0
28	11989.0
29	14593.0
30	17947.5
31	21302.0
32	21302.0
33	27862.5
34	34423.0
35	34423.0
36	38800.0
37	43177.0
38	50278.0
39	57379.0
40	57379.0
41	61232.0
42	65085.0
43	76137.0
44	87189.0
45	87189.0
46	88217.5
47	89246.0
48	89246.0
49	96928.5
50	104611.0
51	107195.5
52	109780.0
53	109780.0
54	104894.0
55	100008.0
56	100008.0
57	96983.5
58	93959.0
59	84575.0
60	75191.0
61	75191.0
62	72116.5
63	69042.0
64	57415.0
65	45788.0
66	45788.0
67	38850.0
68	31912.0
69	31912.0
70	25631.0
71	19350.0
72	14919.0
73	10488.0
74	10488.0
75	7978.0
76	5468.0
77	5468.0
78	5333.0
79	5198.0
80	4118.0
81	3038.0
82	3038.0
83	3149.0
84	3260.0
85	3260.0
86	2114.5
87	969.0
88	847.5
89	726.0
90	726.0
91	508.0
92	290.0
93	238.5
94	187.0
95	187.0
96	170.5
97	154.0
98	154.0
99	324.0
100	494.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1192772576848886
2	0.053798644415736105
3	0.010529668890579928
4	0.002566053763250571
5	1.7696922505176349E-4
6	3.5393845010352697E-4
7	1.7696922505176349E-4
8	6.193922876811723E-4
9	6.193922876811723E-4
10	8.848461252588174E-4
11	0.0030084768258799794
12	0.003273930663457625
13	0.014157538004141079
14	0.009379368927743464
15	0.022652060806625726
16	0.011768453465942273
17	0.019732068593271628
18	0.005839984426708195
19	0.00760967667722583
20	0.007078769002070539
21	0.006370892101863486
22	0.007078769002070539
23	0.010352699665528164
24	0.014334507229192843
25	0.01760843789265047
26	0.01867025324296105
27	0.00858300741501053
28	0.006636345939441131
29	0.010795122728157573
30	0.003804838338612915
31	0.006459376714389368
32	0.006990284389544659
33	0.008494522802484647
34	0.011856938078468155
35	0.016458137929814005
36	0.015484807192029307
37	0.009910276602898756
38	0.006016953651759959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1130140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.1965778900839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4567847155031	37.614121644816244
2	13.805241388804992	13.583412665515098
3	4.542630361442705	6.704456052077282
4	1.881207677731566	3.7019592017977936
5	0.9464495356871861	2.3281039150734197
6	0.5623866069654517	1.660049790834951
7	0.34645795313529604	1.193118197394174
8	0.24707822128434534	0.9724322366686945
9	0.16836049092881206	0.7454484005032859
>10	0.8265364881639323	7.417931226168071
>50	0.09517112937850021	3.3047032870768294
>100	0.09948691834950135	10.848725833016386
>500	0.015527903135889008	5.086372757914397
>1k	0.006680609488696433	4.839164791143371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3782	0.33464880457288476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2619	0.23174120020528433	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2265	0.20041764737112217	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2085	0.18449041711646344	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1744	0.15431716424513778	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1714	0.15166262586936133	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1711	0.15139717203178366	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1669	0.14768081830569663	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1652	0.14617657989275665	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1634	0.14458385686729078	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1613	0.14272568000424726	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1477	0.13069177270072735	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1461	0.12927601890031323	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1446	0.127948749712425	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1433	0.12679844974958854	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1428	0.12635602668695914	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1376	0.12175482683561328	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1357	0.12007361919762152	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1349	0.11936574229741448	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1315	0.1163572654715345	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1283	0.11352575787070629	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1276	0.11290636558302511	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1264	0.11184455023271454	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1206	0.1067124427062134	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1194	0.1056506273559028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.848461252588174E-5	0.0
10	0.0	0.0	0.0	1.7696922505176349E-4	0.0
11	0.0	0.0	0.0	1.7696922505176349E-4	0.0
12	0.0	0.0	0.0	1.7696922505176349E-4	8.848461252588174E-5
13	0.0	0.0	0.0	1.7696922505176349E-4	8.848461252588174E-5
14	0.0	0.0	0.0	1.7696922505176349E-4	1.7696922505176349E-4
15	0.0	0.0	0.0	1.7696922505176349E-4	1.7696922505176349E-4
16	0.0	0.0	0.0	1.7696922505176349E-4	2.6545383757764523E-4
17	0.0	0.0	0.0	1.7696922505176349E-4	2.6545383757764523E-4
18	0.0	0.0	0.0	2.6545383757764523E-4	2.6545383757764523E-4
19	0.0	0.0	0.0	2.6545383757764523E-4	2.6545383757764523E-4
20	0.0	0.0	0.0	3.5393845010352697E-4	2.6545383757764523E-4
21	0.0	0.0	0.0	4.4242306262940877E-4	3.5393845010352697E-4
22	0.0	0.0	0.0	7.078769002070539E-4	3.5393845010352697E-4
23	0.0	0.0	0.0	0.0015042384129399897	3.5393845010352697E-4
24	0.0	0.0	0.0	0.00203514608809528	3.5393845010352697E-4
25	0.0	0.0	0.0	0.0023005999256729254	4.4242306262940877E-4
26	0.0	0.0	0.0	0.003185446050931743	4.4242306262940877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	395	0.0	19.84706	28
CTAGCGG	410	0.0	19.120947	29
TAGCGGC	410	0.0	18.731552	30
GTATTAG	300	0.0	18.679739	1
AAGACGG	535	0.0	18.5378	5
CAAGACG	540	0.0	18.366966	4
GACGGAC	525	0.0	18.283945	7
CGCAAGA	560	0.0	18.004631	2
GCTATAT	45	0.008859634	17.774483	3
AGCGGCG	445	0.0	17.258284	31
GCGCAAG	560	0.0	17.154861	1
CGCTTCG	300	0.0	17.066526	32
ATAATAC	75	3.2387587E-5	17.063505	3
AAACGCT	310	0.0	17.031363	29
ACAATAC	245	0.0	16.976444	3
GTCCTAT	540	0.0	16.900715	1
ACGGACC	560	0.0	16.85551	8
AGAGCGA	580	0.0	16.828192	15
CTATTCC	505	0.0	16.788969	4
GCAAGAC	640	0.0	16.746897	3
>>END_MODULE