FastQCFastQC Report
Thu 2 Feb 2017
SRR4063028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130140
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42920.37977595696108446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30640.27111685277930164No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22190.1963473551949316No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21510.19033040154317166No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18650.16502380236076947No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17740.15697170262091423No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17430.1542286796326119No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17420.154140195020086No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16800.14865414904348132No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16770.1483886952059037No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16700.14776930291822252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16470.14573415683012725No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16100.14246022616666962No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14620.12936450351283912No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14060.12440936521138973No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13940.12334754986107915No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13900.12299361141097565No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13860.12263967296087211No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13780.12193179606066505No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13350.11812695772205213No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13230.11706514237174155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.11175606562018865No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT12340.10919001185693807No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC12320.10901304263188631No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG12250.10839365034420514No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12230.10821668111915338No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC12220.1081281965066275No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12120.10724335038136867No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11940.1056506273559028No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG11920.10547365813085104No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11680.10335002743022989No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11580.10246518130497106No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11340.1003415506043499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4050.020.93798329
TAGCGGC4100.020.68264230
TCTAGCG4300.019.72065728
GTGCAAT507.190081E-419.2022841
CGCAACG507.196506E-419.19973416
AGCGGCG4850.017.81418631
TAGGACG904.3651744E-717.7783184
GAGTAAT550.001363539517.4566211
CGTGTCA550.00136475317.45430410
CAAGACG4500.017.422754
AAGACGG4450.017.2589535
CGCTTCG2650.016.90542632
GCGCAAG4700.016.0019041
TAATACC805.619262E-516.0004884
ATAGGGG600.002441375516.0004863
ATGCGTA600.002442096615.9997822
CGCAAGA4900.015.6732522
TTACACA1652.910383E-1115.5156234
CCCTAGA1255.1286406E-815.3618261
CGGTCCA5750.015.30413610
Produced by FastQC (version 0.11.5)