FastQCFastQC Report
Thu 2 Feb 2017
SRR4063027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292638
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35440.27416801919795025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22410.173366402658749No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16740.12950261403424623No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15840.1225401079033728No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15270.11813052068715293No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14480.11201898752783068No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14390.11132273691474334No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13900.1075320391323789No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13810.10683578851929156No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13760.10644898262313193No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13760.10644898262313193No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13670.10575273201004456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13440.10397342488771026No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13340.10319981309539097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC604.7296307E-621.3293
GTATTAC1105.14774E-1018.922821
GTAGCGT450.00885924217.7748556
CATCGTT3600.016.88872328
TAGTACA1151.8477294E-816.690974
GTATAGG908.5235315E-616.0116161
ATCGTTT3850.015.79205629
CGACGAC1753.6379788E-1215.542706514
TACCGTC3300.015.5150017
ACCGTCG3200.015.499858
CGGCATC4150.015.42153625
GTAATAC1255.1508323E-815.356883
GCATCGT4000.015.19985427
TATTGTC951.4348974E-515.1554035
GTCTTAG1603.0195224E-1015.010891
GCGACGA2150.014.88357732
ATACCGT3550.014.8707946
GTCTAGG1401.2665623E-814.8679291
CGTCGTA3450.014.84043510
GGCATCG4350.014.71250226
Produced by FastQC (version 0.11.5)