##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063027_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292638
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218328720028346	32.0	32.0	32.0	32.0	32.0
2	30.813477555201068	32.0	32.0	32.0	32.0	32.0
3	30.866948828674385	32.0	32.0	32.0	32.0	32.0
4	30.93984239980567	32.0	32.0	32.0	32.0	32.0
5	30.853974585305398	32.0	32.0	32.0	32.0	32.0
6	34.499435263391604	36.0	36.0	36.0	32.0	36.0
7	34.42587406528355	36.0	36.0	36.0	32.0	36.0
8	34.422914226566135	36.0	36.0	36.0	32.0	36.0
9	34.55217315288581	36.0	36.0	36.0	32.0	36.0
10	34.27117104711451	36.0	36.0	36.0	32.0	36.0
11	34.549727766010285	36.0	36.0	36.0	32.0	36.0
12	34.3565429764559	36.0	36.0	36.0	32.0	36.0
13	34.438323026245556	36.0	36.0	36.0	32.0	36.0
14	34.34136548670239	36.0	36.0	36.0	32.0	36.0
15	34.285273216476696	36.0	36.0	36.0	32.0	36.0
16	34.29586782997251	36.0	36.0	36.0	32.0	36.0
17	34.23505111253112	36.0	36.0	36.0	32.0	36.0
18	34.259258972736376	36.0	36.0	36.0	32.0	36.0
19	34.24957799476729	36.0	36.0	36.0	32.0	36.0
20	34.23045972654371	36.0	36.0	36.0	32.0	36.0
21	34.21988135889553	36.0	36.0	36.0	32.0	36.0
22	34.188634404991966	36.0	36.0	36.0	32.0	36.0
23	34.130202732706294	36.0	36.0	36.0	32.0	36.0
24	34.121016092672505	36.0	36.0	36.0	32.0	36.0
25	34.10530945245304	36.0	36.0	36.0	32.0	36.0
26	34.05801856358857	36.0	36.0	36.0	32.0	36.0
27	34.06457956519923	36.0	36.0	36.0	32.0	36.0
28	34.04124743354288	36.0	36.0	36.0	32.0	36.0
29	34.00735936898033	36.0	36.0	36.0	32.0	36.0
30	33.98335574228825	36.0	36.0	36.0	32.0	36.0
31	34.008032411239654	36.0	36.0	36.0	32.0	36.0
32	33.95278415147938	36.0	36.0	36.0	32.0	36.0
33	33.909463438333084	36.0	36.0	36.0	32.0	36.0
34	33.92231699826247	36.0	36.0	36.0	32.0	36.0
35	33.879694082952845	36.0	36.0	36.0	32.0	36.0
36	33.83930226405227	36.0	36.0	36.0	32.0	36.0
37	33.83386299954047	36.0	36.0	36.0	32.0	36.0
38	33.2432111697165	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	3.0
10	10.0
11	16.0
12	4.0
13	10.0
14	159.0
15	346.0
16	471.0
17	561.0
18	680.0
19	808.0
20	1136.0
21	1643.0
22	2540.0
23	3938.0
24	5780.0
25	8854.0
26	12907.0
27	18573.0
28	25813.0
29	34815.0
30	46732.0
31	62036.0
32	84777.0
33	126472.0
34	279128.0
35	574421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59277673771214	17.201566785510412	11.22597015919588	25.979686317581567
2	16.760610259228585	20.084061583237222	37.39687749146612	25.75845066606807
3	18.746160476232475	24.37248448329488	28.722423128285357	28.158931912187285
4	12.583224121417864	15.597705079862664	36.18723280473219	35.63183799398729
5	14.611681392378525	36.538254745918614	33.583132774321015	15.266931087381842
6	33.65322354175321	35.764421555726265	17.08507687035202	13.4972780321685
7	29.728531675946147	30.76772520050099	21.181120453770085	18.322622669782778
8	28.015973585717223	32.47036670859656	19.926290040336244	19.587369665349975
9	27.940524587373854	14.030642834210742	18.52417735865113	39.50465521976428
10	15.900735862952487	26.347870082127013	31.710030387612253	26.041363667308254
11	37.42986117289371	21.184652816190436	22.455487519485693	18.929998491430165
12	24.87209936523532	23.82769678285323	28.846631414856045	22.453572437055403
13	29.641748248123168	19.41451684409167	25.16381412039419	25.779920787390974
14	23.98512365746966	19.79092038665493	25.134813952876623	31.089142002998788
15	25.32022464085474	26.848652170346977	22.516082805751275	25.31504038304701
16	25.84890960309042	25.548099784063734	23.709740187897822	24.893250424948025
17	24.031045539234803	25.529083334880813	25.284504156530673	25.15536696935371
18	25.107170128372058	24.6826673351019	26.11284022309026	24.097322313435782
19	25.751304396264135	24.900429537844513	25.417391331257853	23.930874734633502
20	25.832279227737097	24.11128260474328	24.985513301071897	25.070924866447726
21	27.379090999819738	24.086253436002732	24.24879870458925	24.285856859588275
22	26.084178048595565	24.15650971029271	24.860314449156935	24.898997791954795
23	24.563006518249164	23.93578460764782	25.346124833175377	26.15508404092764
24	24.787385491458284	25.01918791780144	25.181434142114384	25.011992448625897
25	25.06152999824366	24.243941200455875	25.21379788449769	25.480730916802774
26	24.695282124688088	25.21763351838598	25.69394742441534	24.39313693251059
27	25.476641602187332	24.510244612710082	24.87882320992965	25.134290575172933
28	24.63642426221087	24.589154387588216	25.563254209803972	25.211167140396945
29	24.535405145281636	24.847120827156786	25.492292599595828	25.12518142796575
30	24.62053226056011	25.055933776883798	25.73866625406158	24.584867708494507
31	25.23729911099386	24.828654916921135	24.447475450015357	25.486570522069652
32	24.817610296449995	24.85397218856813	24.590541885031435	25.73787562995044
33	24.599416193248853	24.30619758910867	25.256721011395296	25.83766520624718
34	25.351163530211863	24.568642363687427	25.38071890747233	24.69947519862838
35	26.116859493005872	24.377747224051234	25.23001049951216	24.275382783430732
36	24.714985698026815	25.213871946855615	24.853866097630988	25.21727625748658
37	25.65639013279444	24.976325391249986	24.81323364208324	24.55405083387233
38	24.740711170114828	24.740479075085837	25.260913495088094	25.257896259711245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	661.0
1	473.5
2	286.0
3	286.0
4	601.5
5	917.0
6	917.0
7	1060.0
8	1203.0
9	1133.0
10	1063.0
11	1063.0
12	1421.0
13	1779.0
14	2327.5
15	2876.0
16	2876.0
17	4278.5
18	5681.0
19	5681.0
20	6958.0
21	8235.0
22	8856.5
23	9478.0
24	9478.0
25	11377.0
26	13276.0
27	13276.0
28	16885.5
29	20495.0
30	24867.5
31	29240.0
32	29240.0
33	36531.0
34	43822.0
35	43822.0
36	48683.5
37	53545.0
38	61694.5
39	69844.0
40	69844.0
41	74795.0
42	79746.0
43	90386.5
44	101027.0
45	101027.0
46	104022.5
47	107018.0
48	107018.0
49	113253.5
50	119489.0
51	120406.5
52	121324.0
53	121324.0
54	116204.5
55	111085.0
56	111085.0
57	107448.0
58	103811.0
59	93220.5
60	82630.0
61	82630.0
62	78241.5
63	73853.0
64	60714.0
65	47575.0
66	47575.0
67	39662.5
68	31750.0
69	31750.0
70	25661.5
71	19573.0
72	15347.5
73	11122.0
74	11122.0
75	8497.5
76	5873.0
77	5873.0
78	5557.0
79	5241.0
80	4112.0
81	2983.0
82	2983.0
83	3033.5
84	3084.0
85	3084.0
86	2035.5
87	987.0
88	839.0
89	691.0
90	691.0
91	521.5
92	352.0
93	291.0
94	230.0
95	230.0
96	216.0
97	202.0
98	202.0
99	396.5
100	591.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12277219144106859
2	0.05631893848084305
3	0.012377788677108363
4	0.0034038918862047997
5	7.736117923192727E-5
6	5.415282546234909E-4
7	7.736117923192727E-5
8	3.0944471692770907E-4
9	2.320835376957818E-4
10	4.641670753915636E-4
11	0.001779307122334327
12	0.0025529189146535998
13	0.012919316931731855
14	0.008973896790903563
15	0.020423351317228798
16	0.009824869762454762
17	0.016555292355632436
18	0.005183199008539127
19	0.006653061413945746
20	0.0056473660839306905
21	0.005802088442394545
22	0.007117228489337308
23	0.00874181325320778
24	0.012223066318644508
25	0.013847651082514982
26	0.01647793117640051
27	0.008122923819352363
28	0.004641670753915636
29	0.008819174432439708
30	0.0029397248108132363
31	0.005492643725466836
32	0.005879449621626473
33	0.006730422593177673
34	0.011526815705557162
35	0.015240152308689673
36	0.012300427497876435
37	0.008819174432439708
38	0.004951115470843345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1292638.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.433366070455214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64918187923578	45.51304804273637
2	12.015226143191946	13.561193107161095
3	3.346519134650685	5.665660181625753
4	1.3708977745010331	3.0945750381435664
5	0.6973698060085952	1.9677462774482697
6	0.41436693436673383	1.4030472536766108
7	0.27078751584492217	1.0697015706289978
8	0.19069471672856717	0.8609235805475993
9	0.1479354375398657	0.7513645231332183
>10	0.7348482028678512	7.45645829727601
>50	0.07030907435639329	2.8089316489146086
>100	0.07850395420217487	9.399569014162372
>500	0.009778549297783712	3.6342705164142357
>1k	0.003580877207639106	2.81351094813146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3544	0.27416801919795025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2241	0.173366402658749	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1674	0.12950261403424623	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1584	0.1225401079033728	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1527	0.11813052068715293	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1448	0.11201898752783068	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1439	0.11132273691474334	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1390	0.1075320391323789	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1381	0.10683578851929156	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1376	0.10644898262313193	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1376	0.10644898262313193	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1367	0.10575273201004456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1344	0.10397342488771026	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1334	0.10319981309539097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.736117923192727E-5	0.0
8	0.0	0.0	0.0	7.736117923192727E-5	0.0
9	0.0	0.0	0.0	7.736117923192727E-5	0.0
10	0.0	0.0	0.0	7.736117923192727E-5	0.0
11	0.0	0.0	0.0	7.736117923192727E-5	0.0
12	0.0	0.0	0.0	7.736117923192727E-5	7.736117923192727E-5
13	0.0	0.0	0.0	7.736117923192727E-5	3.0944471692770907E-4
14	0.0	0.0	0.0	7.736117923192727E-5	3.0944471692770907E-4
15	0.0	0.0	0.0	7.736117923192727E-5	3.8680589615963635E-4
16	0.0	0.0	0.0	7.736117923192727E-5	3.8680589615963635E-4
17	0.0	0.0	0.0	7.736117923192727E-5	3.8680589615963635E-4
18	0.0	0.0	0.0	7.736117923192727E-5	3.8680589615963635E-4
19	0.0	0.0	0.0	1.5472235846385453E-4	3.8680589615963635E-4
20	0.0	0.0	0.0	2.3208353769578181E-4	4.6416707539156363E-4
21	0.0	0.0	0.0	2.3208353769578181E-4	5.415282546234909E-4
22	0.0	0.0	0.0	3.8680589615963635E-4	5.415282546234909E-4
23	0.0	0.0	0.0	0.0010830565092469818	5.415282546234909E-4
24	0.0	0.0	0.0	0.0013925012261746907	5.415282546234909E-4
25	0.0	0.0	0.0	0.001779307122334327	5.415282546234909E-4
26	0.0	0.0	0.0	0.002320835376957818	5.415282546234909E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTAC	60	4.7296307E-6	21.329	3
GTATTAC	110	5.14774E-10	18.92282	1
GTAGCGT	45	0.008859242	17.774855	6
CATCGTT	360	0.0	16.888723	28
TAGTACA	115	1.8477294E-8	16.69097	4
GTATAGG	90	8.5235315E-6	16.011616	1
ATCGTTT	385	0.0	15.792056	29
CGACGAC	175	3.6379788E-12	15.5427065	14
TACCGTC	330	0.0	15.515001	7
ACCGTCG	320	0.0	15.49985	8
CGGCATC	415	0.0	15.421536	25
GTAATAC	125	5.1508323E-8	15.35688	3
GCATCGT	400	0.0	15.199854	27
TATTGTC	95	1.4348974E-5	15.155403	5
GTCTTAG	160	3.0195224E-10	15.01089	1
GCGACGA	215	0.0	14.883577	32
ATACCGT	355	0.0	14.870794	6
GTCTAGG	140	1.2665623E-8	14.867929	1
CGTCGTA	345	0.0	14.840435	10
GGCATCG	435	0.0	14.712502	26
>>END_MODULE