FastQCFastQC Report
Thu 2 Feb 2017
SRR4063027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292638
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39730.30735596508844704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26850.20771476623772472No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18610.14396915455061665No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16520.12780066809114385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15800.12223066318644507No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15500.11990982780948727No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15400.119136216017168No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15090.11673801946097825No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14800.11449454526325235No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14590.11286996049938189No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14560.1126378769616861No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14530.11240579342399032No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14100.10907926271701744No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13680.10583009318927651No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12990.10049217182227352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3450.018.5497629
GTATAGA953.7911377E-818.526071
TAGCGGC3500.018.28476330
TCTAGCG3500.018.28476328
TAGACTG1505.456968E-1217.069085
CATCGTT3550.016.6751928
CGTTTAT3500.016.45628731
TAGCACT1850.016.4334854
CGAAAAA2550.016.31287824
GTATTAG3050.016.262081
TCGTTTA3700.015.99916830
ATCGTTT3750.015.78584629
CTTACAC1850.015.5685643
ACGAAAA3000.014.39925223
AGCGGCG4350.014.34408231
GGTCTAG901.5345916E-414.2220331
GCATTCG5400.013.92574122
CGACCCG2900.013.7950555
CGGCATC4300.013.766726525
GGCATCG4300.013.766726526
Produced by FastQC (version 0.11.5)