##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292638
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221637457664094	32.0	32.0	32.0	32.0	32.0
2	31.30378419944331	32.0	32.0	32.0	32.0	32.0
3	31.376137789543552	32.0	32.0	32.0	32.0	32.0
4	31.47885177443337	32.0	32.0	32.0	32.0	32.0
5	31.411518924865277	32.0	32.0	32.0	32.0	32.0
6	34.9319933345608	36.0	36.0	36.0	36.0	36.0
7	34.94947618745542	36.0	36.0	36.0	36.0	36.0
8	34.893390106124066	36.0	36.0	36.0	32.0	36.0
9	34.994628813325924	36.0	36.0	36.0	36.0	36.0
10	34.85378814486345	36.0	36.0	36.0	32.0	36.0
11	35.02783145784048	36.0	36.0	36.0	36.0	36.0
12	34.91795614858916	36.0	36.0	36.0	32.0	36.0
13	34.976140265101286	36.0	36.0	36.0	36.0	36.0
14	34.9161397081008	36.0	36.0	36.0	32.0	36.0
15	34.88686778510302	36.0	36.0	36.0	32.0	36.0
16	34.89908079446837	36.0	36.0	36.0	32.0	36.0
17	34.863556540965064	36.0	36.0	36.0	32.0	36.0
18	34.86834365073594	36.0	36.0	36.0	32.0	36.0
19	34.85603161906118	36.0	36.0	36.0	32.0	36.0
20	34.84577120585965	36.0	36.0	36.0	32.0	36.0
21	34.83795231147467	36.0	36.0	36.0	32.0	36.0
22	34.81989311779477	36.0	36.0	36.0	32.0	36.0
23	34.76051145022814	36.0	36.0	36.0	32.0	36.0
24	34.745303016002936	36.0	36.0	36.0	32.0	36.0
25	34.717962028038784	36.0	36.0	36.0	32.0	36.0
26	34.66336824385481	36.0	36.0	36.0	32.0	36.0
27	34.64321024138235	36.0	36.0	36.0	32.0	36.0
28	34.60721253746215	36.0	36.0	36.0	32.0	36.0
29	34.569458734773384	36.0	36.0	36.0	32.0	36.0
30	34.55500147759852	36.0	36.0	36.0	32.0	36.0
31	34.54891934168731	36.0	36.0	36.0	32.0	36.0
32	34.52365550138554	36.0	36.0	36.0	32.0	36.0
33	34.483250531084494	36.0	36.0	36.0	32.0	36.0
34	34.47888194529327	36.0	36.0	36.0	32.0	36.0
35	34.43611204374311	36.0	36.0	36.0	32.0	36.0
36	34.4110129827531	36.0	36.0	36.0	32.0	36.0
37	34.40917874919351	36.0	36.0	36.0	32.0	36.0
38	33.99111429495342	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	26.0
22	91.0
23	316.0
24	858.0
25	1980.0
26	4436.0
27	8882.0
28	14982.0
29	24010.0
30	35855.0
31	51618.0
32	73938.0
33	117759.0
34	288419.0
35	669458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02107067010688	18.38598744347436	11.916088677077571	26.67685320934119
2	15.78593542817092	21.079683561832468	36.70671912786101	26.4276618821356
3	17.726463248024583	25.10323849368501	28.997832339757924	28.17246591853249
4	12.081136593083121	16.45576670908648	36.30344219365869	35.15965450417171
5	14.101627833933398	37.07542250808037	33.27551874538734	15.547430912598886
6	33.704023177463085	36.270486254820575	16.675086161213333	13.350404406502998
7	29.44743747640473	30.93571875058021	21.279606260714573	18.337237512300483
8	27.92065726973124	32.82000897402175	19.6767031300769	19.582630626170104
9	27.78794691555352	13.755258853983731	18.41647183698984	40.04032239347291
10	15.616362053959964	26.73613770428392	31.65450149888792	25.992998742868195
11	37.548353355490775	21.059394940923802	22.352402349431518	19.0398493541539
12	24.71221507745437	23.926840740442756	28.760467016134523	22.60047716596835
13	29.62066719375417	19.36257482760061	25.141686999763273	25.875070978881947
14	23.853236559655528	19.718049446171317	25.128380876935385	31.300333117237773
15	25.209919559845833	26.932134131907	22.418031962544813	25.439914345702352
16	25.797807118317053	25.680991647307017	23.534990875241853	24.98621035913408
17	24.081838844285873	25.669135519766556	25.05713123086278	25.191894405084795
18	25.09202112269638	24.60201541344135	26.046271268522204	24.259692195340072
19	25.794383268943044	24.80431489713284	25.44765046362555	23.953651370298566
20	25.91382892967714	23.962006377655616	25.017290223558337	25.10687446910891
21	27.35483561523025	24.087331488011337	24.289940416419757	24.267892480338656
22	25.88319663570663	24.175443319609418	24.90441996729159	25.036940077392362
23	24.437815440696376	23.824727146698685	25.56233928190148	26.175118130703463
24	24.808260317273668	24.973967963188457	25.1218825378799	25.095889181657977
25	25.039628356032445	24.196080099950876	25.316659381805383	25.4476321622113
26	24.89103677905183	25.05395942251427	25.673235662265846	24.381768136168052
27	25.549652028877425	24.52105619834199	24.786173369765347	25.143118403015237
28	24.551266479865205	24.47498835714253	25.80908189299711	25.16466326999516
29	24.560163015476878	24.599153049809768	25.68468511679217	25.155998817921184
30	24.495411708459756	24.961512813332114	25.87151236463728	24.67156311357085
31	25.23065235587999	24.58236567391644	24.66986116762775	25.517120802575814
32	24.898850258154255	24.791705024918038	24.654234209422903	25.655210507504805
33	24.655936155366003	24.229598696618854	25.317374237798983	25.797090910216163
34	25.301283112518743	24.3498953303245	25.57947391303675	24.76934764412001
35	26.0663851751225	24.201516588557663	25.368896783167443	24.363201453152392
36	24.768109865252296	25.0356248230363	24.926081393243894	25.270183918467502
37	25.74796656140389	24.821024911846937	24.89103677905183	24.539971747697344
38	24.831661813534513	24.55153655011929	25.453956102104534	25.162845534241658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	396.0
1	291.5
2	187.0
3	187.0
4	510.5
5	834.0
6	834.0
7	994.5
8	1155.0
9	1133.5
10	1112.0
11	1112.0
12	1425.0
13	1738.0
14	2316.5
15	2895.0
16	2895.0
17	4498.5
18	6102.0
19	6102.0
20	7263.5
21	8425.0
22	9015.5
23	9606.0
24	9606.0
25	11180.5
26	12755.0
27	12755.0
28	16608.0
29	20461.0
30	24791.0
31	29121.0
32	29121.0
33	36999.5
34	44878.0
35	44878.0
36	49275.0
37	53672.0
38	61840.0
39	70008.0
40	70008.0
41	74887.0
42	79766.0
43	90724.0
44	101682.0
45	101682.0
46	104405.5
47	107129.0
48	107129.0
49	113392.5
50	119656.0
51	120417.5
52	121179.0
53	121179.0
54	116517.0
55	111855.0
56	111855.0
57	108011.0
58	104167.0
59	93472.0
60	82777.0
61	82777.0
62	78275.5
63	73774.0
64	60691.0
65	47608.0
66	47608.0
67	39723.5
68	31839.0
69	31839.0
70	25765.5
71	19692.0
72	15364.0
73	11036.0
74	11036.0
75	8206.0
76	5376.0
77	5376.0
78	5280.5
79	5185.0
80	3961.0
81	2737.0
82	2737.0
83	2627.5
84	2518.0
85	2518.0
86	1619.0
87	720.0
88	588.5
89	457.0
90	457.0
91	270.0
92	83.0
93	59.0
94	35.0
95	35.0
96	22.0
97	9.0
98	9.0
99	11.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006420977876249963
2	0.0
3	0.0
4	2.320835376957818E-4
5	0.0
6	2.320835376957818E-4
7	4.641670753915636E-4
8	0.001392501226174691
9	0.004332226036987927
10	0.0010056953300150546
11	0.005415282546234908
12	4.641670753915636E-4
13	0.0
14	0.0
15	0.0
16	7.736117923192727E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	3.0944471692770907E-4
23	1.5472235846385453E-4
24	0.0
25	2.320835376957818E-4
26	0.0
27	1.5472235846385453E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.5472235846385453E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1292638.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.3737042053812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95903826636996	42.93295391036976
2	13.06861290159937	14.211777845723853
3	3.6561672348285517	5.963980672559225
4	1.4830995238326214	3.225664592640666
5	0.756266633806316	2.0560509123491983
6	0.4603110038272148	1.5017288618749842
7	0.2962255591093004	1.1274816650357935
8	0.20937603435630028	0.9107640447828161
9	0.14846970924880237	0.7265563248737997
>10	0.7868048658906739	7.678960018675745
>50	0.07741360614360596	2.9404943104999934
>100	0.08377554544456797	9.763330602540684
>500	0.010579153961955261	3.845941898982883
>1k	0.003859961580713406	3.1143143390906745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3973	0.30735596508844704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2685	0.20771476623772472	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1861	0.14396915455061665	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1652	0.12780066809114385	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1580	0.12223066318644507	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1550	0.11990982780948727	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1540	0.119136216017168	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1509	0.11673801946097825	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1480	0.11449454526325235	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1459	0.11286996049938189	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1456	0.1126378769616861	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1453	0.11240579342399032	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1410	0.10907926271701744	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1368	0.10583009318927651	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1299	0.10049217182227352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.736117923192727E-5
2	0.0	0.0	0.0	0.0	7.736117923192727E-5
3	0.0	0.0	0.0	0.0	7.736117923192727E-5
4	0.0	0.0	0.0	0.0	7.736117923192727E-5
5	0.0	0.0	0.0	0.0	7.736117923192727E-5
6	0.0	0.0	0.0	0.0	7.736117923192727E-5
7	0.0	0.0	0.0	0.0	7.736117923192727E-5
8	0.0	0.0	0.0	0.0	7.736117923192727E-5
9	0.0	0.0	0.0	0.0	7.736117923192727E-5
10	0.0	0.0	0.0	0.0	7.736117923192727E-5
11	0.0	0.0	0.0	0.0	7.736117923192727E-5
12	0.0	0.0	0.0	0.0	1.5472235846385453E-4
13	0.0	0.0	0.0	0.0	1.5472235846385453E-4
14	0.0	0.0	0.0	0.0	1.5472235846385453E-4
15	0.0	0.0	0.0	0.0	2.3208353769578181E-4
16	0.0	0.0	0.0	0.0	2.3208353769578181E-4
17	0.0	0.0	0.0	0.0	2.3208353769578181E-4
18	0.0	0.0	0.0	0.0	3.0944471692770907E-4
19	0.0	0.0	0.0	7.736117923192727E-5	3.0944471692770907E-4
20	0.0	0.0	0.0	7.736117923192727E-5	6.962506130873454E-4
21	0.0	0.0	0.0	7.736117923192727E-5	6.962506130873454E-4
22	0.0	0.0	0.0	3.8680589615963635E-4	6.962506130873454E-4
23	0.0	0.0	0.0	0.0010830565092469818	6.962506130873454E-4
24	0.0	0.0	0.0	0.0014698624054066182	6.962506130873454E-4
25	0.0	0.0	0.0	0.001779307122334327	6.962506130873454E-4
26	0.0	0.0	0.0	0.002243474197725891	9.283341507831273E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	345	0.0	18.54976	29
GTATAGA	95	3.7911377E-8	18.52607	1
TAGCGGC	350	0.0	18.284763	30
TCTAGCG	350	0.0	18.284763	28
TAGACTG	150	5.456968E-12	17.06908	5
CATCGTT	355	0.0	16.67519	28
CGTTTAT	350	0.0	16.456287	31
TAGCACT	185	0.0	16.433485	4
CGAAAAA	255	0.0	16.312878	24
GTATTAG	305	0.0	16.26208	1
TCGTTTA	370	0.0	15.999168	30
ATCGTTT	375	0.0	15.785846	29
CTTACAC	185	0.0	15.568564	3
ACGAAAA	300	0.0	14.399252	23
AGCGGCG	435	0.0	14.344082	31
GGTCTAG	90	1.5345916E-4	14.222033	1
GCATTCG	540	0.0	13.925741	22
CGACCCG	290	0.0	13.795055	5
CGGCATC	430	0.0	13.7667265	25
GGCATCG	430	0.0	13.7667265	26
>>END_MODULE