FastQCFastQC Report
Thu 2 Feb 2017
SRR4063026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592212
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26880.4538915118234686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17960.3032697750130021No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11690.19739552727739393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10210.17240447677520887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.15315461355055285No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8650.14606255867831114No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7230.12208465887216065No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6530.11026456741842448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.11026456741842448No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6370.10756283222899908No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6360.10739397377965999No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6280.10604310618494728No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG6210.10486109703957366No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6070.10249707874882644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC400.00448177919.9993673
TACCGTC801.2644523E-719.9976797
ATACCGT801.26558E-719.9959896
ACCGTCG751.5077203E-619.1977718
TCTAGCG1055.9681042E-918.28359228
GTTCTAG450.00878259217.799761
ACCGTGT450.00885126817.7757138
TATACTC450.00885557517.7742125
AGTGCAA1006.80011E-817.59944310
TAGCGGC1101.0604708E-817.45399330
TAAGACT550.001365421117.4510444
AGCGGCG1151.8362698E-816.69512431
TAATACT1252.8521754E-916.6366634
CGTTTAT1102.0150583E-715.99949431
TTAATAC805.6150016E-515.9994933
CGTCGTA908.569314E-615.99949310
ATCACGA1407.403287E-1015.99814325
TAATAGT908.583063E-615.9967914
TCACGAA1451.2187229E-915.44648326
CGCTTCG1357.885319E-915.40691932
Produced by FastQC (version 0.11.5)