##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063026_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592212
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159674238279536	32.0	32.0	32.0	32.0	32.0
2	30.83046442827906	32.0	32.0	32.0	32.0	32.0
3	30.904748637312313	32.0	32.0	32.0	32.0	32.0
4	30.931117572761107	32.0	32.0	32.0	32.0	32.0
5	30.888085685531532	32.0	32.0	32.0	32.0	32.0
6	34.50176794796458	36.0	36.0	36.0	32.0	36.0
7	34.41584432601839	36.0	36.0	36.0	32.0	36.0
8	34.396672137680426	36.0	36.0	36.0	32.0	36.0
9	34.50098782192863	36.0	36.0	36.0	32.0	36.0
10	34.272829662350645	36.0	36.0	36.0	32.0	36.0
11	34.532690995792045	36.0	36.0	36.0	32.0	36.0
12	34.36179273638494	36.0	36.0	36.0	32.0	36.0
13	34.440168385645684	36.0	36.0	36.0	32.0	36.0
14	34.355330523528735	36.0	36.0	36.0	32.0	36.0
15	34.30309416222569	36.0	36.0	36.0	32.0	36.0
16	34.320483205338626	36.0	36.0	36.0	32.0	36.0
17	34.2493110575267	36.0	36.0	36.0	32.0	36.0
18	34.275722207587826	36.0	36.0	36.0	32.0	36.0
19	34.252293097742026	36.0	36.0	36.0	32.0	36.0
20	34.21579603250188	36.0	36.0	36.0	32.0	36.0
21	34.18324687780727	36.0	36.0	36.0	32.0	36.0
22	34.1667342100464	36.0	36.0	36.0	32.0	36.0
23	34.126632016912865	36.0	36.0	36.0	32.0	36.0
24	34.131051042532064	36.0	36.0	36.0	32.0	36.0
25	34.101495748144245	36.0	36.0	36.0	32.0	36.0
26	34.047025389556445	36.0	36.0	36.0	32.0	36.0
27	34.055451426178465	36.0	36.0	36.0	32.0	36.0
28	34.04313657946816	36.0	36.0	36.0	32.0	36.0
29	34.017387354528445	36.0	36.0	36.0	32.0	36.0
30	33.95992988997183	36.0	36.0	36.0	32.0	36.0
31	33.97131094945729	36.0	36.0	36.0	32.0	36.0
32	33.940080579252026	36.0	36.0	36.0	32.0	36.0
33	33.872711123719206	36.0	36.0	36.0	32.0	36.0
34	33.868493039654716	36.0	36.0	36.0	32.0	36.0
35	33.81683924000189	36.0	36.0	36.0	32.0	36.0
36	33.78482536659169	36.0	36.0	36.0	32.0	36.0
37	33.78845075749901	36.0	36.0	36.0	32.0	36.0
38	33.14288970841523	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	5.0
11	5.0
12	4.0
13	2.0
14	126.0
15	268.0
16	305.0
17	355.0
18	413.0
19	595.0
20	707.0
21	971.0
22	1337.0
23	1950.0
24	2699.0
25	3909.0
26	5645.0
27	7984.0
28	11203.0
29	15386.0
30	20946.0
31	28388.0
32	38857.0
33	58390.0
34	129463.0
35	262295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.638389808159154	17.13618198170175	11.371996781239222	24.853431428899874
2	16.095635837759666	20.186277689926882	37.86964954249956	25.84843692981389
3	18.640861958320667	25.179180599182626	28.618907690748806	27.561049751747895
4	12.357649442755827	15.717831813576494	36.36001350894968	35.564505234718006
5	14.11622864784908	37.29221967808825	33.49932118903366	15.09223048502901
6	33.16346173329821	35.82332002728752	17.61041654002283	13.402801699391434
7	29.365835207662123	31.2764685619339	21.1816038850952	18.176092345308774
8	27.91881258337414	32.74666081288732	20.01604160686243	19.31848499687611
9	27.798322222447368	14.484340067408294	18.8263662337136	38.890971476430735
10	15.793721821651102	27.10744499417436	31.813545870552673	25.285287313621858
11	36.74917806623089	21.8473119768457	22.440185004753456	18.963324952169955
12	24.786684160845123	24.390532204654868	29.029697887696155	21.79308574680385
13	29.64467262256599	20.00557310050158	25.31842669684022	25.03132758009221
14	23.673460427147347	20.19281918262094	25.991917738587983	30.141802651643722
15	24.93282146470319	27.175089810650267	23.47017881059791	24.42190991404863
16	25.00299755635828	26.147641894185412	24.655787648041287	24.193572901415013
17	23.308692267028768	26.12121918388923	25.95790245245321	24.612186096628786
18	24.170691343848155	25.06264986997197	27.374109223546895	23.392549562632983
19	24.91792869591062	25.460680077275367	26.511733156806855	23.10965807000716
20	25.391225934137935	24.341370907684695	26.348878798062064	23.918524360115303
21	26.720355841357442	24.409210806283287	25.13585533981566	23.73457801254361
22	25.256975532653243	24.81268269453221	25.581034048941447	24.3493077238731
23	23.71723858416644	24.700756552283167	26.261314509592005	25.32069035395839
24	24.21082318926733	25.637691341178375	26.17523676993123	23.97624869962306
25	24.454284908454973	24.883932627724775	26.034388706209793	24.627393757610456
26	23.759227577261697	26.004076938276775	26.570355896107316	23.66633958835421
27	24.63704726955858	25.51080203392006	25.80481189954691	24.04733879697445
28	23.66817241524887	25.269219403048087	26.605479799045888	24.457128382657153
29	23.591955430287207	25.691419924985347	26.676810475706365	24.039814169021078
30	23.939621137667725	25.633491727373126	26.7631890900617	23.663698044897448
31	24.088953284158762	25.702324984464077	25.58833860203723	24.620383129339928
32	23.667039302371922	25.93476961319063	25.635028656924103	24.76316242751335
33	23.469134447324645	25.324022865248708	26.176319100250772	25.030523587175875
34	24.16238974145924	25.705798210193354	26.338935134286164	23.79287691406125
35	24.863497339190324	25.67905112706527	26.09637011854156	23.361081415202843
36	23.73208812179655	26.349227794337438	25.694985096304052	24.22369898756196
37	24.561047979478136	26.235795382581074	25.54171331877619	23.6614433191646
38	23.732969475084943	26.145275359186183	25.970663928723408	24.151091237005463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	766.0
1	545.5
2	325.0
3	325.0
4	654.0
5	983.0
6	983.0
7	1113.5
8	1244.0
9	1158.5
10	1073.0
11	1073.0
12	1358.0
13	1643.0
14	1980.0
15	2317.0
16	2317.0
17	3254.0
18	4191.0
19	4191.0
20	4854.0
21	5517.0
22	5525.0
23	5533.0
24	5533.0
25	6321.5
26	7110.0
27	7110.0
28	8938.0
29	10766.0
30	13147.5
31	15529.0
32	15529.0
33	18653.0
34	21777.0
35	21777.0
36	24025.0
37	26273.0
38	29879.5
39	33486.0
40	33486.0
41	35598.0
42	37710.0
43	42095.5
44	46481.0
45	46481.0
46	49799.0
47	53117.0
48	53117.0
49	54305.5
50	55494.0
51	54254.0
52	53014.0
53	53014.0
54	50441.0
55	47868.0
56	47868.0
57	46022.0
58	44176.0
59	39096.5
60	34017.0
61	34017.0
62	31976.0
63	29935.0
64	24504.5
65	19074.0
66	19074.0
67	15863.5
68	12653.0
69	12653.0
70	10286.5
71	7920.0
72	6071.5
73	4223.0
74	4223.0
75	3228.5
76	2234.0
77	2234.0
78	2174.5
79	2115.0
80	1654.0
81	1193.0
82	1193.0
83	1228.5
84	1264.0
85	1264.0
86	814.5
87	365.0
88	326.0
89	287.0
90	287.0
91	208.5
92	130.0
93	108.5
94	87.0
95	87.0
96	77.0
97	67.0
98	67.0
99	161.0
100	255.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11482374555057985
2	0.05065753480172641
3	0.012157808352414338
4	0.0020263013920690564
5	0.0
6	0.0
7	0.0
8	3.3771689867817604E-4
9	0.0
10	3.3771689867817604E-4
11	0.0018574429427299685
12	0.0025328767400863202
13	0.013846392845805218
14	0.00861178091629349
15	0.02245817376209871
16	0.010638082308362546
17	0.018743287876638773
18	0.005403470378850817
19	0.007429771770919874
20	0.005910045726868081
21	0.006416621074885345
22	0.005910045726868081
23	0.008780639365632578
24	0.01283324214977069
25	0.016716986484569714
26	0.01705470338324789
27	0.009287214713649843
28	0.006247762625546258
29	0.010300365409684369
30	0.0030394520881035845
31	0.006078904176207169
32	0.005741187277528993
33	0.007936347118937138
34	0.01367753439646613
35	0.016716986484569714
36	0.013002100599109778
37	0.010638082308362546
38	0.0067543379735635215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	592212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.0495667029122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.77839018381894	54.30019157044029
2	10.390437951774691	13.310060973293272
3	2.353062733708274	4.521379455563553
4	0.8197392365102961	2.1001577163144214
5	0.3849612330513288	1.2328300087178208
6	0.2487355724515216	0.9558843383472472
7	0.15536211688373475	0.6965613387915287
8	0.10910232138583098	0.5590365128835477
9	0.07879271390504432	0.45419752664681534
>10	0.52652492731718	6.8237554324193646
>50	0.08035302648575704	3.677262524214399
>100	0.06951592004266396	8.575819187610007
>500	0.003964786314218653	1.662987047533824
>1k	0.0010572763504583076	1.1298763672239378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2688	0.4538915118234686	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1796	0.3032697750130021	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1169	0.19739552727739393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1021	0.17240447677520887	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.15315461355055285	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	865	0.14606255867831114	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	723	0.12208465887216065	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	653	0.11026456741842448	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	653	0.11026456741842448	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	637	0.10756283222899908	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	636	0.10739397377965999	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	628	0.10604310618494728	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	621	0.10486109703957366	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	607	0.10249707874882644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.6885844933908802E-4	0.0
11	0.0	0.0	0.0	1.6885844933908802E-4	0.0
12	0.0	0.0	0.0	1.6885844933908802E-4	0.0
13	0.0	0.0	0.0	1.6885844933908802E-4	0.0
14	0.0	0.0	0.0	1.6885844933908802E-4	0.0
15	0.0	0.0	0.0	3.3771689867817604E-4	0.0
16	0.0	0.0	0.0	3.3771689867817604E-4	0.0
17	0.0	0.0	0.0	3.3771689867817604E-4	0.0
18	0.0	0.0	0.0	6.754337973563521E-4	0.0
19	0.0	0.0	0.0	6.754337973563521E-4	0.0
20	0.0	0.0	0.0	0.0011820091453736163	0.0
21	0.0	0.0	0.0	0.0011820091453736163	0.0
22	0.0	0.0	0.0	0.0020263013920690564	0.0
23	0.0	0.0	0.0	0.0033771689867817607	0.0
24	0.0	0.0	0.0	0.005234611929511729	0.0
25	0.0	0.0	0.0	0.005741187277528993	0.0
26	0.0	0.0	0.0	0.0075986302202589616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATAC	40	0.004481779	19.999367	3
TACCGTC	80	1.2644523E-7	19.997679	7
ATACCGT	80	1.26558E-7	19.995989	6
ACCGTCG	75	1.5077203E-6	19.197771	8
TCTAGCG	105	5.9681042E-9	18.283592	28
GTTCTAG	45	0.008782592	17.79976	1
ACCGTGT	45	0.008851268	17.775713	8
TATACTC	45	0.008855575	17.774212	5
AGTGCAA	100	6.80011E-8	17.599443	10
TAGCGGC	110	1.0604708E-8	17.453993	30
TAAGACT	55	0.0013654211	17.451044	4
AGCGGCG	115	1.8362698E-8	16.695124	31
TAATACT	125	2.8521754E-9	16.636663	4
CGTTTAT	110	2.0150583E-7	15.999494	31
TTAATAC	80	5.6150016E-5	15.999493	3
CGTCGTA	90	8.569314E-6	15.999493	10
ATCACGA	140	7.403287E-10	15.998143	25
TAATAGT	90	8.583063E-6	15.996791	4
TCACGAA	145	1.2187229E-9	15.446483	26
CGCTTCG	135	7.885319E-9	15.406919	32
>>END_MODULE