FastQCFastQC Report
Thu 2 Feb 2017
SRR4063026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592212
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34060.5751318784489338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27180.4589572653036412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15160.25598940919805746No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11700.19756438572673302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.19672009348003758No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11220.18945918015845678No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG7180.12124036662546521No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6680.1127974441585108No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6670.11262858570917171No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC6480.10942027517172905No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC6330.10688739843164273No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA6130.10351022944486096No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6110.10317251254618279No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6080.10266593719816552No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6070.10249707874882644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1150.023.65211110
CCGTCGT1200.022.6666059
ATACCGT1300.022.1537886
ACCGTCG1250.021.7599418
TACCGTC1250.021.7599417
GGACCGA453.5340362E-421.3332776
GTCGTAG1400.020.57137311
GTATTAG1301.2732926E-1118.461491
TAGAATA450.00884548517.777734
TGAGTAC450.00884548517.777733
TGTAGGA1352.3646862E-1117.777732
CCTATAC450.00884548517.777733
TTACACA904.3580076E-717.777734
GACGTGG652.0982811E-417.2307247
CGCATCG652.0982811E-417.23072413
GATATAC753.22949E-517.0666221
TGGACAG1700.016.9411335
CGACCAT1700.016.94113321
CCAGTCG854.9758346E-616.94113320
AGGGGCG1900.016.84348332
Produced by FastQC (version 0.11.5)