##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592212
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109114641378426	32.0	32.0	32.0	32.0	32.0
2	31.208862704572013	32.0	32.0	32.0	32.0	32.0
3	31.318475478375987	32.0	32.0	32.0	32.0	32.0
4	31.41831641371671	32.0	32.0	32.0	32.0	32.0
5	31.330239846541442	32.0	32.0	32.0	32.0	32.0
6	34.834932422848574	36.0	36.0	36.0	32.0	36.0
7	34.87687348449542	36.0	36.0	36.0	32.0	36.0
8	34.80826291935996	36.0	36.0	36.0	32.0	36.0
9	34.93673549337062	36.0	36.0	36.0	32.0	36.0
10	34.74820672326802	36.0	36.0	36.0	32.0	36.0
11	34.948830824096774	36.0	36.0	36.0	36.0	36.0
12	34.83248059816417	36.0	36.0	36.0	32.0	36.0
13	34.90806164008835	36.0	36.0	36.0	32.0	36.0
14	34.8277458072447	36.0	36.0	36.0	32.0	36.0
15	34.79906519962446	36.0	36.0	36.0	32.0	36.0
16	34.80213842340243	36.0	36.0	36.0	32.0	36.0
17	34.79006673285918	36.0	36.0	36.0	32.0	36.0
18	34.76772844859611	36.0	36.0	36.0	32.0	36.0
19	34.767904061383426	36.0	36.0	36.0	32.0	36.0
20	34.754361613746426	36.0	36.0	36.0	32.0	36.0
21	34.741817119545026	36.0	36.0	36.0	32.0	36.0
22	34.72410049104037	36.0	36.0	36.0	32.0	36.0
23	34.649463367848	36.0	36.0	36.0	32.0	36.0
24	34.628215909167665	36.0	36.0	36.0	32.0	36.0
25	34.609104509871464	36.0	36.0	36.0	32.0	36.0
26	34.561020377837664	36.0	36.0	36.0	32.0	36.0
27	34.5297663674495	36.0	36.0	36.0	32.0	36.0
28	34.496840658412864	36.0	36.0	36.0	32.0	36.0
29	34.4566945620825	36.0	36.0	36.0	32.0	36.0
30	34.42859651611247	36.0	36.0	36.0	32.0	36.0
31	34.41311726206156	36.0	36.0	36.0	32.0	36.0
32	34.36750859489507	36.0	36.0	36.0	32.0	36.0
33	34.317089150506916	36.0	36.0	36.0	32.0	36.0
34	34.277915678844735	36.0	36.0	36.0	32.0	36.0
35	34.235395432716665	36.0	36.0	36.0	32.0	36.0
36	34.19469379208797	36.0	36.0	36.0	32.0	36.0
37	34.20138565243528	36.0	36.0	36.0	32.0	36.0
38	33.749250268484936	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	11.0
22	59.0
23	162.0
24	424.0
25	1074.0
26	2257.0
27	4605.0
28	7695.0
29	12112.0
30	18400.0
31	26089.0
32	37367.0
33	59164.0
34	138636.0
35	284146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17408852187717	18.660013171893205	12.323825928365165	25.84207237786447
2	15.274935327213903	21.480483340425387	37.1235300871985	26.121051245162207
3	17.820814167899332	25.643857267329945	29.209303425124787	27.32602513964594
4	11.759133553524752	16.73657406469305	36.33107738445016	35.17321499733204
5	13.488244074757013	37.714872376783994	33.41843799180023	15.378445556658765
6	33.04813141239961	36.44252396101396	17.002357263952774	13.506987362633652
7	28.981175660067677	31.35836490986336	21.326822151526816	18.333637278542145
8	27.871861506949475	32.90594610294105	19.850963267663285	19.371229122446188
9	27.502051761813252	14.097039045956086	18.76374173807023	39.63716745416043
10	15.605185349041806	27.34195982148134	31.814044751244076	25.23881007823278
11	36.92312628445115	21.411262473002253	22.520048836584884	19.145562405961712
12	24.583974292946266	24.343857172528036	29.216444814432695	21.855723720093007
13	29.785498749601068	19.827054884154467	25.416447853889913	24.97099851235455
14	23.52586573726976	20.025598940919807	26.035946586695307	30.412588735115126
15	24.870147852458242	27.25510459092352	23.469467015190506	24.405280541427732
16	24.818223265395723	26.078418128704346	24.72467536853481	24.378683237365124
17	23.252990483137793	26.001499463030132	26.177281108792123	24.56822894503995
18	24.32726793783307	24.57954246114567	27.640270713865984	23.452918887155274
19	24.84566337730407	25.407455438255223	26.71222467629835	23.034656508142355
20	25.319817903048232	23.966417431595442	26.70614577212215	24.007618893234177
21	26.599596090589177	24.37437944519868	25.448994616792636	23.577029847419507
22	25.1529018422519	24.563752722851692	26.085510207527733	24.19783522736867
23	23.59492884304945	24.32844994697845	26.728941662782923	25.347679547189184
24	24.089515241163635	25.398168223541568	26.421112709637768	24.09120382565703
25	24.493255793533397	24.43719478835282	26.43107535814877	24.638474059965013
26	23.84133357874136	25.428099106568435	26.89396178051404	23.836605534176165
27	24.711462637472117	25.08008125482303	26.106573501674234	24.10188260603062
28	23.79637697311098	24.853262007524332	26.970746962236497	24.37961405712819
29	23.57247066928735	25.255989409198058	27.104651712562394	24.0668882089522
30	23.897016608917077	25.168858449339087	27.17574111973415	23.758383822009684
31	24.08917752426496	25.108575982925036	26.07968092507413	24.72256556773588
32	23.761760990996468	25.411676899488693	26.153978642783326	24.672583466731506
33	23.56841806650321	24.754648673110307	26.735189425408468	24.941743834978013
34	24.119234328247316	25.108575982925036	26.955043126447965	23.817146562379687
35	25.017561278731264	24.917428218273187	26.57190330489757	23.493107198097977
36	23.864258069745294	25.68117498463388	26.229458369637896	24.225108575982926
37	24.67950666315441	25.729975076492877	25.941723571964094	23.648794688388616
38	23.92462133364854	25.384238699110114	26.43048918457979	24.260650782661557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	386.0
1	284.5
2	183.0
3	183.0
4	592.5
5	1002.0
6	1002.0
7	1132.0
8	1262.0
9	1220.5
10	1179.0
11	1179.0
12	1398.5
13	1618.0
14	2094.5
15	2571.0
16	2571.0
17	3900.5
18	5230.0
19	5230.0
20	5655.5
21	6081.0
22	5916.0
23	5751.0
24	5751.0
25	6403.0
26	7055.0
27	7055.0
28	8822.5
29	10590.0
30	12826.5
31	15063.0
32	15063.0
33	18442.5
34	21822.0
35	21822.0
36	23986.5
37	26151.0
38	29689.0
39	33227.0
40	33227.0
41	35370.5
42	37514.0
43	42196.0
44	46878.0
45	46878.0
46	49748.0
47	52618.0
48	52618.0
49	54259.5
50	55901.0
51	54459.5
52	53018.0
53	53018.0
54	50436.5
55	47855.0
56	47855.0
57	45947.0
58	44039.0
59	39209.0
60	34379.0
61	34379.0
62	32194.0
63	30009.0
64	24630.5
65	19252.0
66	19252.0
67	15851.5
68	12451.0
69	12451.0
70	10157.5
71	7864.0
72	6111.0
73	4358.0
74	4358.0
75	3280.5
76	2203.0
77	2203.0
78	2098.5
79	1994.0
80	1544.5
81	1095.0
82	1095.0
83	1080.0
84	1065.0
85	1065.0
86	672.0
87	279.0
88	232.0
89	185.0
90	185.0
91	113.0
92	41.0
93	30.0
94	19.0
95	19.0
96	15.0
97	11.0
98	11.0
99	12.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007092054872241697
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0015197260440517923
9	0.006416621074885345
10	8.442922466954402E-4
11	0.005572328828189905
12	1.6885844933908802E-4
13	1.6885844933908802E-4
14	0.0
15	0.0
16	3.3771689867817604E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	3.3771689867817604E-4
23	0.0
24	0.0
25	0.0
26	1.6885844933908802E-4
27	1.6885844933908802E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.3771689867817604E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	592212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.24891467900777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87078167966244	52.208651328389955
2	10.930591086950642	13.608348639254267
3	2.548293711909226	4.758855535490683
4	0.8920006595719189	2.2210429180524414
5	0.43044352392212043	1.339732109737976
6	0.25445003549187273	0.9503543129642449
7	0.15845405758937275	0.6904515177996413
8	0.11322707537088889	0.5638610043292835
9	0.0774402986843784	0.43385170909686743
>10	0.5573635237202511	6.949196339866195
>50	0.08402278422190824	3.7463065315910287
>100	0.07692993664218745	9.024689937266075
>500	0.004364819100265146	1.6202123794115384
>1k	0.0016368071625994297	1.8844457367498
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3406	0.5751318784489338	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2718	0.4589572653036412	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1516	0.25598940919805746	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1170	0.19756438572673302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1165	0.19672009348003758	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1122	0.18945918015845678	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	718	0.12124036662546521	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	668	0.1127974441585108	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	667	0.11262858570917171	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	648	0.10942027517172905	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	633	0.10688739843164273	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	613	0.10351022944486096	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	611	0.10317251254618279	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	608	0.10266593719816552	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	607	0.10249707874882644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6885844933908802E-4	0.0
4	0.0	0.0	0.0	1.6885844933908802E-4	0.0
5	0.0	0.0	0.0	1.6885844933908802E-4	0.0
6	0.0	0.0	0.0	1.6885844933908802E-4	0.0
7	0.0	0.0	0.0	1.6885844933908802E-4	0.0
8	0.0	0.0	0.0	1.6885844933908802E-4	0.0
9	0.0	0.0	0.0	1.6885844933908802E-4	0.0
10	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
11	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
12	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
13	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
14	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
15	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
16	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
17	0.0	0.0	1.6885844933908802E-4	1.6885844933908802E-4	0.0
18	0.0	0.0	1.6885844933908802E-4	5.065753480172641E-4	0.0
19	0.0	0.0	1.6885844933908802E-4	5.065753480172641E-4	0.0
20	0.0	0.0	1.6885844933908802E-4	6.754337973563521E-4	0.0
21	0.0	0.0	1.6885844933908802E-4	8.442922466954402E-4	1.6885844933908802E-4
22	0.0	0.0	1.6885844933908802E-4	0.0015197260440517923	1.6885844933908802E-4
23	0.0	0.0	1.6885844933908802E-4	0.0030394520881035845	1.6885844933908802E-4
24	0.0	0.0	1.6885844933908802E-4	0.0050657534801726405	1.6885844933908802E-4
25	0.0	0.0	1.6885844933908802E-4	0.005572328828189905	1.6885844933908802E-4
26	0.0	0.0	1.6885844933908802E-4	0.0075986302202589616	1.6885844933908802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	115	0.0	23.652111	10
CCGTCGT	120	0.0	22.666605	9
ATACCGT	130	0.0	22.153788	6
ACCGTCG	125	0.0	21.759941	8
TACCGTC	125	0.0	21.759941	7
GGACCGA	45	3.5340362E-4	21.333277	6
GTCGTAG	140	0.0	20.571373	11
GTATTAG	130	1.2732926E-11	18.46149	1
TAGAATA	45	0.008845485	17.77773	4
TGAGTAC	45	0.008845485	17.77773	3
TGTAGGA	135	2.3646862E-11	17.77773	2
CCTATAC	45	0.008845485	17.77773	3
TTACACA	90	4.3580076E-7	17.77773	4
GACGTGG	65	2.0982811E-4	17.230724	7
CGCATCG	65	2.0982811E-4	17.230724	13
GATATAC	75	3.22949E-5	17.066622	1
TGGACAG	170	0.0	16.941133	5
CGACCAT	170	0.0	16.941133	21
CCAGTCG	85	4.9758346E-6	16.941133	20
AGGGGCG	190	0.0	16.843483	32
>>END_MODULE