FastQCFastQC Report
Thu 2 Feb 2017
SRR4063025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258235
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38870.3089248033952322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24980.1985320707181091No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14430.114684458785521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14010.11134644959010043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA751.5086534E-619.20019132
CTAGCGG2400.017.33350629
CAACGAT753.2338066E-517.06683730
TAGCGGC2550.016.94134330
CGCTTCG2750.016.87289632
TCTAGCG2550.016.31388728
AACGATT805.621057E-516.0001631
ATACCGT2700.015.9963446
TACCGTC2700.015.4057237
CGAGCCG3250.015.26229815
GTCCTAT3900.015.1905081
AACGCTT2950.015.18659230
GTCCTAC951.422799E-515.16891
GTAAACG3000.014.93288927
CGCAAGA3250.014.7693782
AAACGCT3150.014.73030529
CCTATTC3950.014.5777873
TAAACGC3200.014.50014428
ACGCTTC3200.014.50014431
TCGTTTA2550.014.43151530
Produced by FastQC (version 0.11.5)