##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063025_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258235
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240552043139797	32.0	32.0	32.0	32.0	32.0
2	30.850602629874388	32.0	32.0	32.0	32.0	32.0
3	30.894046819552788	32.0	32.0	32.0	32.0	32.0
4	30.962732716861318	32.0	32.0	32.0	32.0	32.0
5	30.877452940031077	32.0	32.0	32.0	32.0	32.0
6	34.53306973657544	36.0	36.0	36.0	32.0	36.0
7	34.45548009712017	36.0	36.0	36.0	32.0	36.0
8	34.4378220284764	36.0	36.0	36.0	32.0	36.0
9	34.57617615151383	36.0	36.0	36.0	32.0	36.0
10	34.30272166964041	36.0	36.0	36.0	32.0	36.0
11	34.57884020075741	36.0	36.0	36.0	32.0	36.0
12	34.386151235659476	36.0	36.0	36.0	32.0	36.0
13	34.47734485211427	36.0	36.0	36.0	32.0	36.0
14	34.379290831998794	36.0	36.0	36.0	32.0	36.0
15	34.328920273239895	36.0	36.0	36.0	32.0	36.0
16	34.34001120617373	36.0	36.0	36.0	32.0	36.0
17	34.27230048440872	36.0	36.0	36.0	32.0	36.0
18	34.29021049327034	36.0	36.0	36.0	32.0	36.0
19	34.2829360175166	36.0	36.0	36.0	32.0	36.0
20	34.2578397517157	36.0	36.0	36.0	32.0	36.0
21	34.24432955687928	36.0	36.0	36.0	32.0	36.0
22	34.2293617646942	36.0	36.0	36.0	32.0	36.0
23	34.16803538289747	36.0	36.0	36.0	32.0	36.0
24	34.16544683624283	36.0	36.0	36.0	32.0	36.0
25	34.14327291801611	36.0	36.0	36.0	32.0	36.0
26	34.100498714469076	36.0	36.0	36.0	32.0	36.0
27	34.10584350300222	36.0	36.0	36.0	32.0	36.0
28	34.094335318918965	36.0	36.0	36.0	32.0	36.0
29	34.05547532853561	36.0	36.0	36.0	32.0	36.0
30	34.0273923392689	36.0	36.0	36.0	32.0	36.0
31	34.04803077326572	36.0	36.0	36.0	32.0	36.0
32	34.00379420378546	36.0	36.0	36.0	32.0	36.0
33	33.959727713821344	36.0	36.0	36.0	32.0	36.0
34	33.96756090873326	36.0	36.0	36.0	32.0	36.0
35	33.93793846141619	36.0	36.0	36.0	32.0	36.0
36	33.88305284783844	36.0	36.0	36.0	32.0	36.0
37	33.88878707077772	36.0	36.0	36.0	32.0	36.0
38	33.306225784531506	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	5.0
9	7.0
10	15.0
11	18.0
12	10.0
13	10.0
14	132.0
15	315.0
16	381.0
17	431.0
18	588.0
19	821.0
20	1061.0
21	1562.0
22	2455.0
23	3641.0
24	5605.0
25	8512.0
26	12169.0
27	17489.0
28	24470.0
29	32842.0
30	44411.0
31	59204.0
32	80899.0
33	121548.0
34	269872.0
35	569759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.157543310445035	17.137893963712408	11.39133735397073	25.313225371871823
2	16.62754284932718	19.446525216572592	37.53158930170348	26.39434263239675
3	18.969420263129447	23.808827363427678	28.484493930472272	28.737258442970603
4	12.519263554031337	15.29448698741461	36.23924559193454	35.94700386661951
5	14.57759777211198	36.560109741287775	33.667479447351525	15.194813039248722
6	34.072094812403435	35.575871540383645	16.946318326032085	13.40571532118083
7	30.276164846920366	30.743248076271986	20.697104036292135	18.283483040515517
8	28.210308465556324	32.718394440435276	19.42930851960058	19.64198857440782
9	27.46294397685638	14.429635281307867	18.77550209421171	39.33191864762404
10	15.856280305541052	26.626594406255787	31.664000216177207	25.853125072025957
11	37.221516185462214	21.460073422524452	22.26424001964716	19.054170372366173
12	24.727444692155764	24.029693566526387	28.662398613876338	22.580463127441515
13	29.226489034442736	19.608594389640945	25.36934731286217	25.79556926305414
14	23.435810939705714	19.8814716841958	25.59558253106281	31.087134845035674
15	25.145181639024187	26.914787818177338	22.846442543049687	25.09358799974879
16	25.740777540200465	26.065401766197425	23.695024919122147	24.498795774479955
17	23.888844283582696	26.015324969970916	25.37761924786974	24.71821149857665
18	24.69111270958435	25.056809827166187	26.29792273545577	23.954154727793696
19	25.13273984579922	25.31213734997218	25.944042603926555	23.61108020030204
20	25.62964328890507	24.326839849205133	25.43514874818481	24.60836811370499
21	26.817991420620874	24.360779027336537	24.850949780349136	23.970279771693452
22	25.55519169811561	24.531445231153686	25.39519157094213	24.518171499788576
23	24.22070546454038	24.400819335295562	25.846737880267995	25.531737319896063
24	24.384111540357072	25.395065261283605	25.606588130554364	24.614235067804962
25	24.724519986708916	24.725712368818094	25.646231357105727	24.903536287367267
26	24.438973514303296	25.57287104061967	25.933295282369418	24.054860162707616
27	25.058878233533765	25.1781846507247	25.119048092170367	24.643889023571163
28	24.150207053325172	24.961410982966783	26.21907116116777	24.669310802540277
29	24.131054085762706	25.418655152788862	26.06503899605429	24.38525176539414
30	24.307732414336854	25.377846165584682	26.13171046524197	24.182710954836494
31	24.814229883613997	25.314014883864367	24.96262342615017	24.909131806371466
32	24.370828607377813	25.187918828286264	25.105176520896606	25.336076043439316
33	24.183508902731667	24.8916419004258	25.56694754061094	25.357901656231597
34	24.94608715558774	25.015480319129065	25.78134873919855	24.257083786084642
35	25.38740371545084	24.8567953640331	25.77651650649051	23.97928441402555
36	24.266444730079165	25.567142574384928	25.372709589775017	24.79370310576089
37	25.25610372598332	25.356494478906015	25.12924415221877	24.258157642891888
38	24.260457086719942	25.25541632025638	25.641064363164094	24.843062229859587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	153.5
2	177.0
3	177.0
4	505.5
5	834.0
6	834.0
7	1021.0
8	1208.0
9	1237.0
10	1266.0
11	1266.0
12	1664.5
13	2063.0
14	2680.0
15	3297.0
16	3297.0
17	4851.0
18	6405.0
19	6405.0
20	7867.0
21	9329.0
22	9953.5
23	10578.0
24	10578.0
25	12425.5
26	14273.0
27	14273.0
28	18144.5
29	22016.0
30	26256.5
31	30497.0
32	30497.0
33	37402.5
34	44308.0
35	44308.0
36	49247.5
37	54187.0
38	61204.0
39	68221.0
40	68221.0
41	74156.5
42	80092.0
43	90122.5
44	100153.0
45	100153.0
46	103675.5
47	107198.0
48	107198.0
49	112452.0
50	117706.0
51	117790.0
52	117874.0
53	117874.0
54	112380.5
55	106887.0
56	106887.0
57	102490.0
58	98093.0
59	87207.0
60	76321.0
61	76321.0
62	71172.0
63	66023.0
64	54869.0
65	43715.0
66	43715.0
67	36609.0
68	29503.0
69	29503.0
70	23780.5
71	18058.0
72	14149.5
73	10241.0
74	10241.0
75	7799.5
76	5358.0
77	5358.0
78	4934.0
79	4510.0
80	3559.5
81	2609.0
82	2609.0
83	2525.0
84	2441.0
85	2441.0
86	1632.0
87	823.0
88	706.5
89	590.0
90	590.0
91	435.5
92	281.0
93	237.0
94	193.0
95	193.0
96	186.5
97	180.0
98	180.0
99	388.5
100	597.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12334738741173151
2	0.05475924608678029
3	0.011683032183971993
4	0.0023842922824432638
5	2.3842922824432637E-4
6	2.3842922824432637E-4
7	7.947640941477546E-5
8	5.563348659034282E-4
9	3.973820470738773E-4
10	6.358112753182037E-4
11	0.0030201035577614673
12	0.003973820470738773
13	0.01629266393002897
14	0.01104722090865379
15	0.02606826228804635
16	0.013113607553437951
17	0.022889205911455334
18	0.007152876847329792
19	0.010729315270994686
20	0.008106593760307097
21	0.008106593760307097
22	0.008821881445040076
23	0.011285650136898116
24	0.017087428024176725
25	0.01978962594427909
26	0.0216970597702337
27	0.01033193322392081
28	0.008345022988551424
29	0.012636749096949298
30	0.004053296880153549
31	0.007470782484988892
32	0.008901357854454851
33	0.010967744499239013
34	0.014782612151148235
35	0.019471720306619988
36	0.01732585725242105
37	0.012000937821631094
38	0.0073913060755741175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1258235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.25024287154301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.291170556533	46.187299424869884
2	13.146244693138526	15.315438924481054
3	3.7405887044504325	6.536706015503643
4	1.4443844746196774	3.365429857859291
5	0.7144584934792905	2.08086903834027
6	0.38347613826286403	1.3402546913551945
7	0.23549269670916387	0.9602254744448391
8	0.16476802908462895	0.767822216931608
9	0.11821907054579417	0.619766061420653
>10	0.6105850503803417	6.523546551551622
>50	0.06676603237243045	2.7311412282775662
>100	0.0753379627175653	9.116227553202508
>500	0.00617523220619303	2.5466581973728313
>1k	0.0023328655001173667	1.908614764389061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3887	0.3089248033952322	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2498	0.1985320707181091	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1443	0.114684458785521	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1401	0.11134644959010043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.947640941477546E-5
2	0.0	0.0	0.0	0.0	7.947640941477546E-5
3	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
4	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
5	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
6	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
7	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
8	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
9	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
10	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
11	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
12	0.0	0.0	0.0	7.947640941477546E-5	7.947640941477546E-5
13	0.0	0.0	0.0	7.947640941477546E-5	1.5895281882955092E-4
14	0.0	0.0	0.0	7.947640941477546E-5	1.5895281882955092E-4
15	0.0	0.0	0.0	7.947640941477546E-5	2.3842922824432637E-4
16	0.0	0.0	0.0	1.5895281882955092E-4	2.3842922824432637E-4
17	0.0	0.0	0.0	1.5895281882955092E-4	2.3842922824432637E-4
18	0.0	0.0	0.0	1.5895281882955092E-4	3.1790563765910184E-4
19	0.0	0.0	0.0	1.5895281882955092E-4	3.1790563765910184E-4
20	0.0	7.947640941477546E-5	0.0	1.5895281882955092E-4	3.1790563765910184E-4
21	0.0	7.947640941477546E-5	0.0	3.973820470738773E-4	3.1790563765910184E-4
22	0.0	7.947640941477546E-5	0.0	5.563348659034282E-4	3.1790563765910184E-4
23	0.0	1.5895281882955092E-4	0.0	0.0015100517788807337	3.1790563765910184E-4
24	0.0	1.5895281882955092E-4	0.0	0.002463768691858039	3.1790563765910184E-4
25	0.0	1.5895281882955092E-4	0.0	0.0028611507389319163	3.1790563765910184E-4
26	0.0	1.5895281882955092E-4	0.0	0.003973820470738773	3.1790563765910184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	75	1.5086534E-6	19.200191	32
CTAGCGG	240	0.0	17.333506	29
CAACGAT	75	3.2338066E-5	17.066837	30
TAGCGGC	255	0.0	16.941343	30
CGCTTCG	275	0.0	16.872896	32
TCTAGCG	255	0.0	16.313887	28
AACGATT	80	5.621057E-5	16.00016	31
ATACCGT	270	0.0	15.996344	6
TACCGTC	270	0.0	15.405723	7
CGAGCCG	325	0.0	15.262298	15
GTCCTAT	390	0.0	15.190508	1
AACGCTT	295	0.0	15.186592	30
GTCCTAC	95	1.422799E-5	15.1689	1
GTAAACG	300	0.0	14.932889	27
CGCAAGA	325	0.0	14.769378	2
AAACGCT	315	0.0	14.730305	29
CCTATTC	395	0.0	14.577787	3
TAAACGC	320	0.0	14.500144	28
ACGCTTC	320	0.0	14.500144	31
TCGTTTA	255	0.0	14.431515	30
>>END_MODULE