FastQCFastQC Report
Thu 2 Feb 2017
SRR4063025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258235
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41780.3320524385349318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30470.24216461948682083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17650.1402758626170787No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14320.11381021828195846No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14120.11222069009366295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.10562414811223658No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13260.10538571888399224No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12970.10308090301096379No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12680.10077608713793529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2600.018.46096629
TCTAGCG2700.017.77722728
ACACCGC550.00136393717.4560856
TAGCGGC2800.017.14232430
CGCAAGA2950.016.2713282
GGACCGA600.002440641216.0014116
CAAGACG3450.015.7695074
CGGTCCA3450.015.76825310
GCGCAAG2950.015.7308251
TAGACTG1753.6379788E-1215.5442285
AAGACGG3450.015.3056985
CATCGTT2050.014.82880828
AGCGGCG3350.014.8055131
GGCGTTA2100.014.4757431
TTTCGTT3000.014.39955328
AGGCCCG4150.014.26518410
ATCGTTT2150.014.13909629
TACCGTC2950.014.1029397
GTGTAGG1952.910383E-1113.9505021
ATACCGT3100.013.9367126
Produced by FastQC (version 0.11.5)