FastQCFastQC Report
Thu 2 Feb 2017
SRR4063024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328265
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43530.3277207484952175No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30360.22856884733091662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28850.21720063390964905No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26510.19958366741576417No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22800.17165249404298089No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22150.166758892239124No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21740.16367215879361424No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21660.1630698693408318No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21560.16231700752485384No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20820.15674583008661674No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20160.15177694210116205No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19390.14597990611813155No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC19080.14364603448859978No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19040.1433448897622086No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG19010.1431190312174152No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18610.14010758395350326No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17960.13521398214964633No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17790.13393411706248376No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17010.12806179489785546No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16920.1273842192634753No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16440.12377048254678097No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16280.12256590364121617No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC15590.11737115711096806No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG15380.1157901472974143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15160.11413385130226272No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT14430.10863796004562341No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14420.10856267386402563No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14390.10833681531923223No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14250.10728280877686305No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC14090.10607822987129827No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG14010.10547594041851588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.10540065423691809No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC13960.10509950951052688No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13820.1040455029681577No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG13660.10284092406259292No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13590.10231392079140834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCG802.8005252E-617.9975227
TCTAGCG5350.017.94077928
TAGTACA450.00886376417.7733234
CTAGCGG5450.017.6135829
CGAACTC550.001365367717.45345720
ATCGTTT4250.016.94011929
GTTCTAC1051.1720658E-716.783611
TAGCGGC5750.016.6946130
CATCGTT4150.016.57540728
TCGTTTA4400.015.63538730
AGCGGCG6250.015.61502631
CGCAAGA6100.015.2196152
AAACGCT3900.015.1785429
TCGCGTA3300.015.0310649
AACGCTT3950.014.98640730
AAGACGG6200.014.9639925
CGTCTAT756.1842444E-414.952671
GCGGCGC6550.014.89983532
TATACAG2150.014.8799925
CAAGACG6050.014.8062074
Produced by FastQC (version 0.11.5)