##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063024_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328265
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24926577151397	32.0	32.0	32.0	32.0	32.0
2	30.95354993167779	32.0	32.0	32.0	32.0	32.0
3	31.00531068724991	32.0	32.0	32.0	32.0	32.0
4	31.02460728845524	32.0	32.0	32.0	32.0	32.0
5	31.02214994748789	32.0	32.0	32.0	32.0	32.0
6	34.59435353638017	36.0	36.0	36.0	32.0	36.0
7	34.53745675749944	36.0	36.0	36.0	32.0	36.0
8	34.518629942067285	36.0	36.0	36.0	32.0	36.0
9	34.624221070343644	36.0	36.0	36.0	32.0	36.0
10	34.37900720112327	36.0	36.0	36.0	32.0	36.0
11	34.616602108765946	36.0	36.0	36.0	32.0	36.0
12	34.46298216093927	36.0	36.0	36.0	32.0	36.0
13	34.55060850056277	36.0	36.0	36.0	32.0	36.0
14	34.42875405133765	36.0	36.0	36.0	32.0	36.0
15	34.40466774325906	36.0	36.0	36.0	32.0	36.0
16	34.41695821240491	36.0	36.0	36.0	32.0	36.0
17	34.32840886419502	36.0	36.0	36.0	32.0	36.0
18	34.37097190696134	36.0	36.0	36.0	32.0	36.0
19	34.352922797785084	36.0	36.0	36.0	32.0	36.0
20	34.305905071653626	36.0	36.0	36.0	32.0	36.0
21	34.28566137028379	36.0	36.0	36.0	32.0	36.0
22	34.28353302993002	36.0	36.0	36.0	32.0	36.0
23	34.23645206340602	36.0	36.0	36.0	32.0	36.0
24	34.23071826781553	36.0	36.0	36.0	32.0	36.0
25	34.197638272483275	36.0	36.0	36.0	32.0	36.0
26	34.162465321302605	36.0	36.0	36.0	32.0	36.0
27	34.15999066451348	36.0	36.0	36.0	32.0	36.0
28	34.15218348748179	36.0	36.0	36.0	32.0	36.0
29	34.12557960949058	36.0	36.0	36.0	32.0	36.0
30	34.09593040545373	36.0	36.0	36.0	32.0	36.0
31	34.105888508693674	36.0	36.0	36.0	32.0	36.0
32	34.065328831219674	36.0	36.0	36.0	32.0	36.0
33	34.018646881458146	36.0	36.0	36.0	32.0	36.0
34	34.02429786224887	36.0	36.0	36.0	32.0	36.0
35	33.98579500325613	36.0	36.0	36.0	32.0	36.0
36	33.94836873666023	36.0	36.0	36.0	32.0	36.0
37	33.96226129575047	36.0	36.0	36.0	32.0	36.0
38	33.2563773042277	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	3.0
10	12.0
11	9.0
12	8.0
13	13.0
14	216.0
15	407.0
16	582.0
17	735.0
18	937.0
19	1236.0
20	1660.0
21	2042.0
22	2760.0
23	3753.0
24	5274.0
25	7662.0
26	10739.0
27	15434.0
28	21973.0
29	30345.0
30	42277.0
31	59008.0
32	84075.0
33	133333.0
34	305982.0
35	597785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70373067219638	17.31450095013685	11.297922362399756	25.683846015267015
2	17.310954627905435	19.37752728412248	36.53368105147072	26.777837036501367
3	19.137649977599832	23.177134510189255	27.97566475794641	29.7095507542645
4	12.534463053949832	14.65698062239033	35.410326794826815	37.39822952883302
5	15.148355408529786	35.875876991228395	32.96816970735465	16.007597892887176
6	35.27496834208914	34.989331923972834	16.223154769164516	13.512544964773515
7	31.266652362292163	30.027968816463584	20.246185813824802	18.459193007419454
8	29.04873364496887	31.66877594087307	19.064400746540024	20.218089667618035
9	27.412232987342094	13.879952810479265	18.77944169105319	39.928372511125445
10	16.571165713915732	25.468075126914254	30.17604247364407	27.78471668552594
11	38.54145335180389	20.918056977654643	21.449587423959528	19.09090224658194
12	25.460482282019566	23.488680775198816	27.58120411646503	23.469632826316587
13	29.22498516624349	18.527135985639116	25.441850168517497	26.806028679599898
14	24.0037164868658	19.600025900961953	24.374612238505694	32.02164537366655
15	25.531000008283637	26.8776935942629	21.92483125853309	25.66647513892037
16	26.54131030105255	25.374235660229182	23.086694069077264	24.997759969641002
17	24.321238942717876	25.707865608511028	24.543081136560964	25.427814312210128
18	25.851125824623434	23.88610898205216	25.847737734738164	24.41502745858625
19	25.685351805142492	24.472461694838685	25.31513759740993	24.527048902608893
20	26.126209557195907	23.407134015775068	24.495697846363274	25.97095858066576
21	27.83484430162546	23.517874500160747	24.111018335658578	24.536262862555215
22	26.4551666812231	23.412696022479153	24.99329902030171	25.13883827599604
23	24.355168179186336	23.33900794872818	25.829790149660763	26.47603372242472
24	24.942453870857225	24.620029893115174	25.163602685109538	25.273913550918063
25	25.06268759214888	24.025047928711597	25.141451822044043	25.770812657095483
26	24.856418887119304	25.537822055052917	25.507174349301014	24.09858470852676
27	25.80718511836908	24.07129099925158	24.692613847699104	25.428910034680236
28	24.56925889339967	24.112768187348664	26.022003108021828	25.295969811229835
29	24.403346994278365	24.461399108662864	25.949521013585397	25.185732883473367
30	24.276819526738592	24.96954593707415	26.038186157517902	24.715448378669358
31	25.26410101002496	24.80829101367656	24.22486325323656	25.702744723061922
32	24.94142307298474	24.46753723926491	24.153043892037754	26.43799579571259
33	24.288250600842083	24.02735864399429	25.28859996265443	26.3957907925092
34	25.663765457616222	24.126945402603937	25.19646975642042	25.012819383359425
35	26.34377753179569	23.79613105323002	25.440546381427854	24.419545033546434
36	24.414005290499315	25.0869513817721	24.770168993276563	25.728874334452023
37	25.94793982759049	24.848413204559538	24.6733533265518	24.53029364129817
38	24.51902118467644	24.364222539616332	25.34918162263012	25.767574653077112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	134.0
2	197.0
3	197.0
4	487.5
5	778.0
6	778.0
7	1044.5
8	1311.0
9	1331.0
10	1351.0
11	1351.0
12	1697.5
13	2044.0
14	2783.5
15	3523.0
16	3523.0
17	4946.5
18	6370.0
19	6370.0
20	7487.0
21	8604.0
22	8614.5
23	8625.0
24	8625.0
25	9747.5
26	10870.0
27	10870.0
28	14113.0
29	17356.0
30	21506.0
31	25656.0
32	25656.0
33	34064.0
34	42472.0
35	42472.0
36	47112.0
37	51752.0
38	60599.0
39	69446.0
40	69446.0
41	73308.5
42	77171.0
43	89483.5
44	101796.0
45	101796.0
46	102351.5
47	102907.0
48	102907.0
49	111039.0
50	119171.0
51	123242.0
52	127313.0
53	127313.0
54	121578.0
55	115843.0
56	115843.0
57	112858.0
58	109873.0
59	98025.5
60	86178.0
61	86178.0
62	84492.5
63	82807.0
64	69266.0
65	55725.0
66	55725.0
67	47196.5
68	38668.0
69	38668.0
70	30901.0
71	23134.0
72	17865.5
73	12597.0
74	12597.0
75	9591.5
76	6586.0
77	6586.0
78	6645.5
79	6705.0
80	5285.0
81	3865.0
82	3865.0
83	3965.5
84	4066.0
85	4066.0
86	2670.0
87	1274.0
88	1048.5
89	823.0
90	823.0
91	597.0
92	371.0
93	295.0
94	219.0
95	219.0
96	205.5
97	192.0
98	192.0
99	373.5
100	555.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12151189709884701
2	0.05669049474314237
3	0.011292927239669794
4	0.002333871629531758
5	1.5057236319559726E-4
6	2.258585447933959E-4
7	0.0
8	3.011447263911945E-4
9	3.011447263911945E-4
10	4.517170895867918E-4
11	0.0018821545399449657
12	0.002860874900716348
13	0.016035956680331108
14	0.009711917426116023
15	0.026049018832838326
16	0.011895216692452184
17	0.022134137389752798
18	0.0062487530726172864
19	0.008657910883746843
20	0.007001614888595272
21	0.00662518398060628
22	0.007603904341377662
23	0.009636631244518225
24	0.015057236319559726
25	0.018896831581047456
26	0.02032726903140563
27	0.00986248978931162
28	0.0067004701622040775
29	0.011142354876474197
30	0.002635016355922952
31	0.0060228945278238905
32	0.007302759614986468
33	0.009711917426116023
34	0.01490666395636413
35	0.01919797630743865
36	0.01686410467790689
37	0.011142354876474197
38	0.00662518398060628
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1328265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.519485843431355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70035200625	34.00321615647835
2	14.318723610990663	13.035618734129914
3	5.047513039125044	6.8928059498696275
4	2.1407690827708614	3.8978683182897496
5	1.0998729805418657	2.503282628367405
6	0.6377734836140228	1.7418672635210644
7	0.4191705292804638	1.3356298881495987
8	0.26820951583213903	0.9767007407195704
9	0.19472542509780846	0.7977421107986259
>10	0.9391035628052857	7.645888162561411
>50	0.09536448821751727	3.088585425024569
>100	0.10990895054272971	11.192303168684399
>500	0.01906414166943362	5.949567738407865
>1k	0.009449183262241012	6.938923714997909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4353	0.3277207484952175	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3036	0.22856884733091662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2885	0.21720063390964905	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2651	0.19958366741576417	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2280	0.17165249404298089	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2215	0.166758892239124	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2174	0.16367215879361424	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2166	0.1630698693408318	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2156	0.16231700752485384	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2082	0.15674583008661674	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2016	0.15177694210116205	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1939	0.14597990611813155	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1908	0.14364603448859978	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1904	0.1433448897622086	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1901	0.1431190312174152	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1861	0.14010758395350326	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1796	0.13521398214964633	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1779	0.13393411706248376	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1701	0.12806179489785546	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1692	0.1273842192634753	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1644	0.12377048254678097	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1628	0.12256590364121617	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1559	0.11737115711096806	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1538	0.1157901472974143	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1516	0.11413385130226272	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1443	0.10863796004562341	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1442	0.10856267386402563	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1439	0.10833681531923223	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1425	0.10728280877686305	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1409	0.10607822987129827	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1401	0.10547594041851588	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1400	0.10540065423691809	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1396	0.10509950951052688	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1382	0.1040455029681577	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1366	0.10284092406259292	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1359	0.10231392079140834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5057236319559726E-4
2	0.0	0.0	0.0	0.0	1.5057236319559726E-4
3	0.0	0.0	0.0	0.0	1.5057236319559726E-4
4	0.0	0.0	0.0	0.0	1.5057236319559726E-4
5	0.0	0.0	0.0	0.0	1.5057236319559726E-4
6	0.0	0.0	0.0	0.0	1.5057236319559726E-4
7	0.0	0.0	0.0	7.528618159779863E-5	1.5057236319559726E-4
8	0.0	0.0	0.0	7.528618159779863E-5	1.5057236319559726E-4
9	0.0	0.0	0.0	7.528618159779863E-5	1.5057236319559726E-4
10	0.0	0.0	0.0	7.528618159779863E-5	3.011447263911945E-4
11	0.0	0.0	0.0	7.528618159779863E-5	3.011447263911945E-4
12	0.0	0.0	0.0	7.528618159779863E-5	6.02289452782389E-4
13	0.0	0.0	0.0	7.528618159779863E-5	7.528618159779863E-4
14	0.0	0.0	0.0	7.528618159779863E-5	7.528618159779863E-4
15	0.0	0.0	0.0	7.528618159779863E-5	9.787203607713822E-4
16	0.0	0.0	0.0	7.528618159779863E-5	9.787203607713822E-4
17	0.0	0.0	0.0	7.528618159779863E-5	9.787203607713822E-4
18	0.0	0.0	0.0	7.528618159779863E-5	0.001204578905564778
19	0.0	0.0	0.0	1.5057236319559726E-4	0.0012798650871625767
20	0.0	0.0	0.0	1.5057236319559726E-4	0.0013551512687603755
21	0.0	0.0	0.0	2.258585447933959E-4	0.0013551512687603755
22	0.0	0.0	0.0	5.270032711845905E-4	0.0013551512687603755
23	0.0	0.0	0.0	8.28147997575785E-4	0.0013551512687603755
24	0.0	0.0	0.0	0.001204578905564778	0.0013551512687603755
25	0.0	0.0	0.0	0.0013551512687603755	0.0013551512687603755
26	0.0	0.0	0.0	0.002032726903140563	0.001430437450358174
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTGCG	80	2.8005252E-6	17.997522	7
TCTAGCG	535	0.0	17.940779	28
TAGTACA	45	0.008863764	17.773323	4
CTAGCGG	545	0.0	17.61358	29
CGAACTC	55	0.0013653677	17.453457	20
ATCGTTT	425	0.0	16.940119	29
GTTCTAC	105	1.1720658E-7	16.78361	1
TAGCGGC	575	0.0	16.69461	30
CATCGTT	415	0.0	16.575407	28
TCGTTTA	440	0.0	15.635387	30
AGCGGCG	625	0.0	15.615026	31
CGCAAGA	610	0.0	15.219615	2
AAACGCT	390	0.0	15.17854	29
TCGCGTA	330	0.0	15.031064	9
AACGCTT	395	0.0	14.986407	30
AAGACGG	620	0.0	14.963992	5
CGTCTAT	75	6.1842444E-4	14.95267	1
GCGGCGC	655	0.0	14.899835	32
TATACAG	215	0.0	14.879992	5
CAAGACG	605	0.0	14.806207	4
>>END_MODULE