FastQCFastQC Report
Thu 2 Feb 2017
SRR4063024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328265
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45630.3435308466307551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33570.25273571162381No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT29930.2253315415222113No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26720.2011646772293179No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24050.1810632667427057No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23060.17360993476452363No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23010.17323350385653463No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21900.164876737699179No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21850.16450030679119002No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21670.16314515552242964No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21230.1598325635321265No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21110.15892912935295292No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19480.14665748175251173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18800.14153802140386143No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17790.13393411706248376No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17580.13235310724892999No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17500.1317508177961476No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG17160.12919108762182246No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17030.12821236726105106No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17020.12813708107945326No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16780.12633021272110612No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16660.12542677854193252No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16280.12256590364121617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.1200814596484888No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15930.11993088728529322No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15830.11917802546931523No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC15650.11782287420055484No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15520.11684415383978349No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG15070.11345627566788255No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT14990.11285398621510015No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14950.11255284148870895No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC14700.11067068694876399No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14490.10908967713521023No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC13990.10532536805532028No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC13700.10314206878898413No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC13570.10216334842821276No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG13330.10035648006986558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG453.5352592E-421.3350415
TCTAGCG4350.019.4936128
CTAGCGG4350.019.4936129
ACACCGA507.192874E-419.2015366
CAAGACG5800.017.6565844
ACCGTCG4600.017.3926948
ATAATAC652.0994301E-417.2321473
TAGCGGC5050.017.10834730
AAGACGG6000.017.0680315
GACTATA753.232085E-517.0680311
TACCGTC4600.017.0448427
ATCGTTT4450.016.8983229
CGCGGTC2750.016.87280710
TAAACGC2950.016.81300528
AGAACCG3150.016.7632475
CGCTTCG2900.016.55117832
TCGTTTA4550.016.52692830
CGCAAGA6200.016.5162072
TAGAACC3800.016.4223674
GCGCAAG6150.016.3915561
Produced by FastQC (version 0.11.5)