##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328265
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146587465603627	32.0	32.0	32.0	32.0	32.0
2	31.17381471317847	32.0	32.0	32.0	32.0	32.0
3	31.30063654466541	32.0	32.0	32.0	32.0	32.0
4	31.421756200758132	32.0	32.0	32.0	32.0	32.0
5	31.304205109673145	32.0	32.0	32.0	32.0	32.0
6	34.840416633728964	36.0	36.0	36.0	32.0	36.0
7	34.87908813376849	36.0	36.0	36.0	32.0	36.0
8	34.805588493260004	36.0	36.0	36.0	32.0	36.0
9	34.94515175812056	36.0	36.0	36.0	32.0	36.0
10	34.751993013442345	36.0	36.0	36.0	32.0	36.0
11	34.95595984235074	36.0	36.0	36.0	36.0	36.0
12	34.83113008322887	36.0	36.0	36.0	32.0	36.0
13	34.90322337786511	36.0	36.0	36.0	32.0	36.0
14	34.83673137513975	36.0	36.0	36.0	32.0	36.0
15	34.80994906889815	36.0	36.0	36.0	32.0	36.0
16	34.8183811212371	36.0	36.0	36.0	32.0	36.0
17	34.76401621664352	36.0	36.0	36.0	32.0	36.0
18	34.77605146563374	36.0	36.0	36.0	32.0	36.0
19	34.762643749552986	36.0	36.0	36.0	32.0	36.0
20	34.75424256454849	36.0	36.0	36.0	32.0	36.0
21	34.74908395538541	36.0	36.0	36.0	32.0	36.0
22	34.743691959059376	36.0	36.0	36.0	32.0	36.0
23	34.677005717985494	36.0	36.0	36.0	32.0	36.0
24	34.655338354921646	36.0	36.0	36.0	32.0	36.0
25	34.632434792755966	36.0	36.0	36.0	32.0	36.0
26	34.557107203758285	36.0	36.0	36.0	32.0	36.0
27	34.55371179696822	36.0	36.0	36.0	32.0	36.0
28	34.53208358271881	36.0	36.0	36.0	32.0	36.0
29	34.48037176316473	36.0	36.0	36.0	32.0	36.0
30	34.46483118955931	36.0	36.0	36.0	32.0	36.0
31	34.47234324475914	36.0	36.0	36.0	32.0	36.0
32	34.433999992471385	36.0	36.0	36.0	32.0	36.0
33	34.39102739287718	36.0	36.0	36.0	32.0	36.0
34	34.374594678019825	36.0	36.0	36.0	32.0	36.0
35	34.349287228075724	36.0	36.0	36.0	32.0	36.0
36	34.31934440793064	36.0	36.0	36.0	32.0	36.0
37	34.32107297866013	36.0	36.0	36.0	32.0	36.0
38	33.841594109609154	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	30.0
22	100.0
23	287.0
24	823.0
25	2104.0
26	4377.0
27	8928.0
28	15953.0
29	25061.0
30	39084.0
31	57159.0
32	84223.0
33	137756.0
34	328944.0
35	623430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99324091830749	18.66340797160538	12.075995057746216	26.267356052340908
2	16.263772665846048	20.34646700771307	35.73955498338058	27.650205343060307
3	18.021027430520263	23.969313352380738	28.375964133663086	29.633695083435914
4	12.040094499428577	15.553783816746996	35.54419233554826	36.86192934827617
5	14.675998144947089	36.54589750230376	32.37722320261634	16.400881150132804
6	35.28362186762431	35.52288135274211	15.78788871196636	13.405608067667222
7	31.023885491780938	30.29684081508507	20.242137422511057	18.43713627062294
8	28.961363968313	31.923098351053497	18.861246670410964	20.254291010222538
9	27.425229859268608	13.677793471484803	18.461170121457247	40.43580654778934
10	16.433365581157233	25.679933446514415	30.17861781058607	27.708083161742287
11	38.84895173761743	20.72070579838247	21.346817266702704	19.08352519729739
12	25.23764136894679	23.615220491138047	27.43141036933978	23.715727770575384
13	29.305491457640542	18.51079191395078	25.45233888168232	26.731377746726366
14	23.909009120920903	19.603919398613982	24.258111144989893	32.22896033547523
15	25.49446081918894	26.962240215619627	21.823657176843476	25.719641788347957
16	26.57895826510523	25.40042837837329	22.9343542139558	25.086259142565677
17	24.457318381497668	25.75991989550278	24.33723692184918	25.44552480115037
18	25.86682627337165	24.004923716276497	25.726793975599747	24.4014560347521
19	25.804489315008677	24.49661776829172	25.23193790395742	24.466955012742186
20	26.308906731713925	23.270130583881983	24.531926987461087	25.889035696943
21	27.918094595652672	23.53854354254877	24.03159311703095	24.511768744767608
22	26.433018159807208	23.498584241223313	25.03126263398137	25.037134964988105
23	24.32421241243276	23.271034018061155	25.866374556282068	26.538379013224016
24	25.048465479403582	24.57724926878296	25.140013476226507	25.234271775586947
25	25.186182867261326	24.022709340914155	25.06188528786446	25.72922250396006
26	25.03890413434066	25.349986636702766	25.467809510903322	24.14329971805325
27	25.97328093415094	24.013506340978648	24.59448980436886	25.41872292050156
28	24.650653295840815	24.00341799264454	26.074013845128796	25.27191486638585
29	24.543897490335137	24.26880178277678	25.930518383003392	25.256782343884694
30	24.4596522531272	24.680316051390346	26.08214475274136	24.777886942741095
31	25.33733855819434	24.613461922131503	24.32699800115188	25.72220151852228
32	25.147767952931076	24.32067396189766	24.27437296021502	26.25718512495624
33	24.48570127196004	23.922184202700517	25.300749473937806	26.291365051401637
34	25.679438967374736	24.044448961615338	25.253168607167996	25.02294346384193
35	26.398196143088914	23.714544913853786	25.42173436776547	24.465524575291827
36	24.622797408649628	24.894731096580877	24.765238864232664	25.71723263053683
37	26.13273706677508	24.67775632121602	24.55918058519949	24.63032602680941
38	24.843461206912774	23.93438056411936	25.452300557494173	25.76985767147369
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	52.5
2	79.0
3	79.0
4	306.0
5	533.0
6	533.0
7	798.0
8	1063.0
9	1186.0
10	1309.0
11	1309.0
12	1685.5
13	2062.0
14	2776.5
15	3491.0
16	3491.0
17	5142.5
18	6794.0
19	6794.0
20	7651.0
21	8508.0
22	8680.0
23	8852.0
24	8852.0
25	9843.5
26	10835.0
27	10835.0
28	13979.0
29	17123.0
30	21206.5
31	25290.0
32	25290.0
33	34034.5
34	42779.0
35	42779.0
36	47266.5
37	51754.0
38	60577.5
39	69401.0
40	69401.0
41	72988.0
42	76575.0
43	89472.5
44	102370.0
45	102370.0
46	102322.0
47	102274.0
48	102274.0
49	111223.0
50	120172.0
51	123429.5
52	126687.0
53	126687.0
54	121509.0
55	116331.0
56	116331.0
57	113546.0
58	110761.0
59	99010.5
60	87260.0
61	87260.0
62	85418.0
63	83576.0
64	69633.0
65	55690.0
66	55690.0
67	47271.5
68	38853.0
69	38853.0
70	31238.0
71	23623.0
72	18040.0
73	12457.0
74	12457.0
75	9474.0
76	6491.0
77	6491.0
78	6514.5
79	6538.0
80	5047.5
81	3557.0
82	3557.0
83	3470.5
84	3384.0
85	3384.0
86	2175.0
87	966.0
88	786.5
89	607.0
90	607.0
91	365.0
92	123.0
93	78.5
94	34.0
95	34.0
96	26.5
97	19.0
98	19.0
99	18.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009486058881322628
2	0.0
3	0.0
4	2.258585447933959E-4
5	7.528618159779863E-5
6	0.0
7	8.28147997575785E-4
8	0.0017315821767493686
9	0.004592457077465717
10	7.528618159779863E-4
11	0.005044174167052508
12	2.258585447933959E-4
13	1.5057236319559726E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	7.528618159779863E-5
22	1.5057236319559726E-4
23	0.0
24	0.0
25	7.528618159779863E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1328265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.78280569155042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0938605067066	32.00254291810426
2	15.245849204661537	13.350121066607493
3	5.275145364675147	6.928819934887646
4	2.2893320448601697	4.009335203342104
5	1.1591310047799122	2.537500377666525
6	0.7110372353447781	1.8678723068734575
7	0.4541496401981658	1.391876181617868
8	0.2958344615912522	1.0361970198971386
9	0.21623053925765484	0.852046171640736
>10	1.0130662201794471	7.897091410352202
>50	0.09891343352744478	3.0933478927918383
>100	0.1158168045443609	11.255929997249362
>500	0.021434748194130267	6.436186911665516
>1k	0.010198791479465208	7.3411326073039085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4563	0.3435308466307551	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3357	0.25273571162381	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2993	0.2253315415222113	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2672	0.2011646772293179	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2405	0.1810632667427057	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2306	0.17360993476452363	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2301	0.17323350385653463	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2190	0.164876737699179	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2185	0.16450030679119002	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2167	0.16314515552242964	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2123	0.1598325635321265	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2111	0.15892912935295292	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1948	0.14665748175251173	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1880	0.14153802140386143	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1779	0.13393411706248376	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1758	0.13235310724892999	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1750	0.1317508177961476	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1716	0.12919108762182246	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1703	0.12821236726105106	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1702	0.12813708107945326	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1678	0.12633021272110612	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1666	0.12542677854193252	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1628	0.12256590364121617	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.1200814596484888	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1593	0.11993088728529322	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1583	0.11917802546931523	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1565	0.11782287420055484	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1552	0.11684415383978349	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1507	0.11345627566788255	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1499	0.11285398621510015	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1495	0.11255284148870895	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1470	0.11067068694876399	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1449	0.10908967713521023	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1399	0.10532536805532028	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1370	0.10314206878898413	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1357	0.10216334842821276	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1333	0.10035648006986558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.528618159779863E-5
7	0.0	0.0	0.0	0.0	7.528618159779863E-5
8	0.0	0.0	0.0	0.0	7.528618159779863E-5
9	0.0	0.0	0.0	0.0	7.528618159779863E-5
10	0.0	0.0	0.0	0.0	7.528618159779863E-5
11	0.0	0.0	0.0	0.0	7.528618159779863E-5
12	0.0	0.0	0.0	0.0	2.258585447933959E-4
13	0.0	0.0	0.0	0.0	3.7643090798899316E-4
14	0.0	0.0	0.0	0.0	3.7643090798899316E-4
15	0.0	0.0	0.0	0.0	5.270032711845905E-4
16	0.0	0.0	0.0	0.0	5.270032711845905E-4
17	0.0	0.0	0.0	0.0	5.270032711845905E-4
18	0.0	0.0	0.0	7.528618159779863E-5	5.270032711845905E-4
19	0.0	0.0	0.0	7.528618159779863E-5	5.270032711845905E-4
20	0.0	0.0	0.0	7.528618159779863E-5	6.02289452782389E-4
21	0.0	0.0	0.0	1.5057236319559726E-4	6.02289452782389E-4
22	0.0	0.0	0.0	3.7643090798899316E-4	6.02289452782389E-4
23	0.0	0.0	0.0	6.775756343801877E-4	6.02289452782389E-4
24	0.0	0.0	0.0	9.787203607713822E-4	6.02289452782389E-4
25	0.0	0.0	0.0	0.0011292927239669795	6.02289452782389E-4
26	0.0	0.0	7.528618159779863E-5	0.0015810098135537712	6.775756343801877E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	45	3.5352592E-4	21.335041	5
TCTAGCG	435	0.0	19.49361	28
CTAGCGG	435	0.0	19.49361	29
ACACCGA	50	7.192874E-4	19.201536	6
CAAGACG	580	0.0	17.656584	4
ACCGTCG	460	0.0	17.392694	8
ATAATAC	65	2.0994301E-4	17.232147	3
TAGCGGC	505	0.0	17.108347	30
AAGACGG	600	0.0	17.068031	5
GACTATA	75	3.232085E-5	17.068031	1
TACCGTC	460	0.0	17.044842	7
ATCGTTT	445	0.0	16.89832	29
CGCGGTC	275	0.0	16.872807	10
TAAACGC	295	0.0	16.813005	28
AGAACCG	315	0.0	16.763247	5
CGCTTCG	290	0.0	16.551178	32
TCGTTTA	455	0.0	16.526928	30
CGCAAGA	620	0.0	16.516207	2
TAGAACC	380	0.0	16.422367	4
GCGCAAG	615	0.0	16.391556	1
>>END_MODULE