FastQCFastQC Report
Thu 2 Feb 2017
SRR4063023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800386
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33030.412675883886025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23280.29085966021394677No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15480.1934066812762842No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12930.16154705354666374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12350.1543005499846324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.13043706411656375No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8690.10857261371388305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA350.002066769622.8556732
AAGACCG555.8361526E-520.3610525
TAGCGTA902.03745E-819.5542967
AGGGACG2200.018.1806478
GACGGCC2250.017.77774211
ACGTGAA450.00885127517.7766328
AACGATT450.00885127517.77663231
GCATTCG2400.017.33221622
CGTATAT957.685376E-716.84207210
ATAGCGT1151.841181E-816.6935336
CTAGCGG1456.91216E-1116.55065729
TCTAGCG1456.91216E-1116.54962328
ACAATAC1551.0913936E-1116.5160983
CCCTCGA1551.0913936E-1116.51506426
TGTAATA1203.0890078E-816.006972
CGTATTG2500.015.99797127
AGGACGT805.62354E-515.9979695
AGGCCCG2050.015.60972610
CGCGCAA1451.2187229E-915.4472821
TAAGACC1451.2205419E-915.4463154
Produced by FastQC (version 0.11.5)