##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063023_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800386
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148945633731724	32.0	32.0	32.0	32.0	32.0
2	30.733989600017992	32.0	32.0	32.0	32.0	32.0
3	30.79181419964867	32.0	32.0	32.0	32.0	32.0
4	30.862452616612483	32.0	32.0	32.0	32.0	32.0
5	30.753377745237923	32.0	32.0	32.0	32.0	32.0
6	34.405335675536556	36.0	36.0	36.0	32.0	36.0
7	34.32096888251419	36.0	36.0	36.0	32.0	36.0
8	34.29783504459098	36.0	36.0	36.0	32.0	36.0
9	34.446986828855074	36.0	36.0	36.0	32.0	36.0
10	34.14644059241416	36.0	36.0	36.0	32.0	36.0
11	34.46574902609491	36.0	36.0	36.0	32.0	36.0
12	34.25605895155587	36.0	36.0	36.0	32.0	36.0
13	34.35556718883139	36.0	36.0	36.0	32.0	36.0
14	34.24437958684935	36.0	36.0	36.0	32.0	36.0
15	34.19462109532151	36.0	36.0	36.0	32.0	36.0
16	34.2042264607327	36.0	36.0	36.0	32.0	36.0
17	34.136455410264546	36.0	36.0	36.0	32.0	36.0
18	34.162925138620615	36.0	36.0	36.0	32.0	36.0
19	34.14367817528043	36.0	36.0	36.0	32.0	36.0
20	34.11884140902015	36.0	36.0	36.0	32.0	36.0
21	34.095513914536234	36.0	36.0	36.0	32.0	36.0
22	34.06652165330228	36.0	36.0	36.0	32.0	36.0
23	34.01828242872814	36.0	36.0	36.0	32.0	36.0
24	33.99860317396856	36.0	36.0	36.0	32.0	36.0
25	33.97485213384542	36.0	36.0	36.0	32.0	36.0
26	33.93933302181697	36.0	36.0	36.0	32.0	36.0
27	33.94816501038249	36.0	36.0	36.0	32.0	36.0
28	33.926832803172466	36.0	36.0	36.0	32.0	36.0
29	33.88529034740738	36.0	36.0	36.0	32.0	36.0
30	33.85177776722731	36.0	36.0	36.0	32.0	36.0
31	33.84704255196868	36.0	36.0	36.0	32.0	36.0
32	33.81590632519809	36.0	36.0	36.0	32.0	36.0
33	33.76103405107036	36.0	36.0	36.0	32.0	36.0
34	33.76473476547566	36.0	36.0	36.0	32.0	36.0
35	33.70645038768794	36.0	36.0	36.0	27.0	36.0
36	33.653715832111004	36.0	36.0	36.0	27.0	36.0
37	33.65015380079112	36.0	36.0	36.0	27.0	36.0
38	33.033278942910044	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	6.0
12	4.0
13	2.0
14	170.0
15	304.0
16	397.0
17	437.0
18	573.0
19	684.0
20	932.0
21	1228.0
22	1807.0
23	2659.0
24	3960.0
25	5871.0
26	8523.0
27	12149.0
28	16942.0
29	22631.0
30	30595.0
31	41299.0
32	55344.0
33	82167.0
34	176223.0
35	335472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.722580661301336	17.27203816604833	11.609673602233524	25.39570757041681
2	16.006565180542466	20.567065594303845	38.25355197267925	25.172817252474445
3	18.583072288328633	25.39331683137388	28.826115912728707	27.197494967568776
4	12.301873873963922	16.05674347242664	36.691471736962974	34.94991091664647
5	14.023876009670348	37.2073439657165	33.902434453419296	14.866345571193861
6	32.65091174872093	36.284663005928394	17.687113076831775	13.377312168518902
7	28.900305602546773	31.399474753431466	21.724392980386963	17.9758266636348
8	27.821735789651232	32.89791787702168	20.237260818231224	19.04308551509586
9	27.83869283070918	14.352200063469375	18.705224729068224	39.10388237675322
10	15.491775218457093	27.409649843950294	32.32615263135537	24.77242230623724
11	36.51108978779973	21.65969494337661	22.628489609883353	19.2007256589403
12	24.46559291730231	24.33015606473482	29.626811499138526	21.57743951882435
13	29.782796677096556	20.101991342856856	25.369628022752494	24.745583957294095
14	23.56445713957269	20.039184962770538	26.10848087046596	30.287877027190817
15	24.850597236142598	27.13781545790604	23.746353972701346	24.26523333325002
16	24.976539666394682	26.216219931099715	24.614419576976758	24.19282082552884
17	23.424667463122272	26.01385632807306	26.124077119563317	24.437399089241353
18	24.298737289808606	25.13300564008886	27.234357312249013	23.33389975785352
19	24.924561331169194	25.686757405484563	26.356615299649267	23.032065963696976
20	25.211691545707502	24.323193046946358	26.453415365238982	24.011700042107158
21	26.459096809984743	24.730208748520944	25.18888823638924	23.621806205105074
22	25.198512111893983	24.874895199349268	25.65482445344352	24.271768235313225
23	23.77900591145192	24.805107014648055	26.242548209383603	25.17333886451642
24	23.994861689219668	25.876618061812177	25.968462867832198	24.160057381135953
25	24.484437852002454	24.896062820918146	26.084201923665685	24.535297403413715
26	23.95820186022216	25.593362883281866	26.57295992262196	23.875475333874014
27	24.705556013704484	25.581078615857116	25.71452490372625	23.99884046671215
28	23.954474291518967	25.208567869365357	26.466784699983382	24.37017313913229
29	23.75491374549235	25.800381354132046	26.471874508000692	23.972830392374917
30	23.930361685433127	25.723670729817933	26.559665352264723	23.786302232484218
31	24.234136077507255	25.55445324350462	25.59681034762813	24.614600331360002
32	23.749661074341237	25.81670150676664	25.714117924887823	24.719519494004295
33	23.588337092269917	25.26030375539631	26.260659865178088	24.890699287155684
34	24.293970273090213	25.615249567341635	26.283136632574646	23.80764352699351
35	24.88294297664108	25.577225145610566	26.11805546009939	23.421776417648964
36	23.9683053403827	26.172336275290185	25.79408467987319	24.065273704453922
37	24.652789492032433	26.070870303521026	25.673522785631015	23.60281741881553
38	23.881314996057913	26.024662111085707	25.827745062392466	24.266277830463917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	643.0
1	489.0
2	335.0
3	335.0
4	741.0
5	1147.0
6	1147.0
7	1254.0
8	1361.0
9	1276.5
10	1192.0
11	1192.0
12	1572.5
13	1953.0
14	2431.5
15	2910.0
16	2910.0
17	4178.0
18	5446.0
19	5446.0
20	6319.0
21	7192.0
22	7325.0
23	7458.0
24	7458.0
25	8626.5
26	9795.0
27	9795.0
28	12389.5
29	14984.0
30	18184.5
31	21385.0
32	21385.0
33	25381.5
34	29378.0
35	29378.0
36	32822.5
37	36267.0
38	41241.0
39	46215.0
40	46215.0
41	49782.5
42	53350.0
43	59177.0
44	65004.0
45	65004.0
46	69151.0
47	73298.0
48	73298.0
49	74836.0
50	76374.0
51	73941.0
52	71508.0
53	71508.0
54	67832.5
55	64157.0
56	64157.0
57	61091.0
58	58025.0
59	51733.5
60	45442.0
61	45442.0
62	42132.5
63	38823.0
64	31962.0
65	25101.0
66	25101.0
67	20650.5
68	16200.0
69	16200.0
70	13057.0
71	9914.0
72	7794.0
73	5674.0
74	5674.0
75	4268.5
76	2863.0
77	2863.0
78	2700.5
79	2538.0
80	1967.0
81	1396.0
82	1396.0
83	1423.0
84	1450.0
85	1450.0
86	958.5
87	467.0
88	402.0
89	337.0
90	337.0
91	251.5
92	166.0
93	151.5
94	137.0
95	137.0
96	123.0
97	109.0
98	109.0
99	250.5
100	392.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1164438158588481
2	0.05097540436739273
3	0.009620358177179511
4	0.0014992765990409628
5	1.2493971658674688E-4
6	1.2493971658674688E-4
7	0.0
8	1.2493971658674688E-4
9	0.0
10	0.0
11	0.001249397165867469
12	0.0016242163156277095
13	0.01524264542358312
14	0.009870237610353005
15	0.024987943317349375
16	0.012369031942087943
17	0.02173951068609396
18	0.005497347529816863
19	0.008370961011312041
20	0.005872166679577104
21	0.00562228724640361
22	0.007121563845444573
23	0.009370478744006018
24	0.015367585140169868
25	0.017866379471904805
26	0.019865414937292755
27	0.0079961418615518
28	0.005872166679577104
29	0.009995177326939752
30	0.0027486737649084314
31	0.005997106396163851
32	0.007871202144965055
33	0.00887071987765903
34	0.012618911375261437
35	0.01686686173921083
36	0.015617464573343363
37	0.009870237610353005
38	0.006122046112750599
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.6213761384782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.99814751693748	55.12074033144333
2	10.972011267701443	14.399969567869144
3	2.562789980486223	5.045214158202289
4	0.9096746825592494	2.3877641803148504
5	0.4155119853156453	1.3633234139221893
6	0.24908524204788007	0.9807189815380692
7	0.14958574234871785	0.687121558453309
8	0.10812747287498901	0.5676378854746202
9	0.06817979777912418	0.40266469395983595
>10	0.4401746655466147	5.625761641435203
>50	0.06221166425501533	2.953972898605498
>100	0.059729273419349506	7.613505462316576
>500	0.003625738633456511	1.505319973095738
>1k	0.0011449700947757404	1.3462852533692937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3303	0.412675883886025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2328	0.29085966021394677	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1548	0.1934066812762842	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1293	0.16154705354666374	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1235	0.1543005499846324	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1044	0.13043706411656375	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	869	0.10857261371388305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.4987943317349376E-4	0.0
8	0.0	0.0	0.0	2.4987943317349376E-4	0.0
9	0.0	0.0	0.0	2.4987943317349376E-4	0.0
10	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	0.0
11	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	0.0
12	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	3.748191497602407E-4
13	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	6.246985829337345E-4
14	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	6.246985829337345E-4
15	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	6.246985829337345E-4
16	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	6.246985829337345E-4
17	1.2493971658674688E-4	0.0	0.0	3.748191497602407E-4	6.246985829337345E-4
18	1.2493971658674688E-4	0.0	0.0	4.997588663469875E-4	6.246985829337345E-4
19	1.2493971658674688E-4	0.0	0.0	6.246985829337345E-4	6.246985829337345E-4
20	1.2493971658674688E-4	0.0	1.2493971658674688E-4	7.496382995204814E-4	8.745780161072283E-4
21	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.0016242163156277097	9.99517732693975E-4
22	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.002498794331734938	9.99517732693975E-4
23	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.003373372347842166	9.99517732693975E-4
24	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.004872648946883129	9.99517732693975E-4
25	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.005997106396163851	9.99517732693975E-4
26	1.2493971658674688E-4	0.0	1.2493971658674688E-4	0.007371443278618067	0.0011244574492807221
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	35	0.0020667696	22.85567	32
AAGACCG	55	5.8361526E-5	20.361052	5
TAGCGTA	90	2.03745E-8	19.554296	7
AGGGACG	220	0.0	18.180647	8
GACGGCC	225	0.0	17.777742	11
ACGTGAA	45	0.008851275	17.776632	8
AACGATT	45	0.008851275	17.776632	31
GCATTCG	240	0.0	17.332216	22
CGTATAT	95	7.685376E-7	16.842072	10
ATAGCGT	115	1.841181E-8	16.693533	6
CTAGCGG	145	6.91216E-11	16.550657	29
TCTAGCG	145	6.91216E-11	16.549623	28
ACAATAC	155	1.0913936E-11	16.516098	3
CCCTCGA	155	1.0913936E-11	16.515064	26
TGTAATA	120	3.0890078E-8	16.00697	2
CGTATTG	250	0.0	15.997971	27
AGGACGT	80	5.62354E-5	15.997969	5
AGGCCCG	205	0.0	15.609726	10
CGCGCAA	145	1.2187229E-9	15.44728	21
TAAGACC	145	1.2205419E-9	15.446315	4
>>END_MODULE