FastQCFastQC Report
Thu 2 Feb 2017
SRR4063023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800386
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40480.5057559727431514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29810.3724452951450925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17770.22201787637464923No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15990.1997786068222083No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14960.18690981601377335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.1580487414822348No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8590.10732321654801558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT453.533235E-421.3353351
TCTAGCG1950.018.87120628
CTAGCGG1950.018.87120629
TAGCGGC2050.017.9506630
CCCTATA450.00884958417.7772222
TCTATTC550.001363298717.456183
TCTTATG854.981599E-616.9406472
GCATTCG2250.016.35504522
CTATACT703.6920098E-416.00154
CTTATAC1102.0147672E-716.00153
ATAACAC600.002439510316.00153
CGCCGGT2000.016.00157
CCCGCCG1407.403287E-1016.00158
CGTATTG2300.015.99949927
CGGCGGG1357.899871E-915.40692631
GGCGGGT1357.899871E-915.40692632
TATTGCG2400.015.33285329
CGGTCCA2400.015.33285310
TACCGTC1153.3375E-715.3057827
TCTTGCG2300.015.3038692
Produced by FastQC (version 0.11.5)