##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800386
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129568733086288	32.0	32.0	32.0	32.0	32.0
2	31.25285549722259	32.0	32.0	32.0	32.0	32.0
3	31.35800601209916	32.0	32.0	32.0	32.0	32.0
4	31.459634476365153	32.0	32.0	32.0	32.0	32.0
5	31.37590112770588	32.0	32.0	32.0	32.0	32.0
6	34.88861624266291	36.0	36.0	36.0	36.0	36.0
7	34.91845184698383	36.0	36.0	36.0	36.0	36.0
8	34.862954874273164	36.0	36.0	36.0	32.0	36.0
9	34.96459958070231	36.0	36.0	36.0	32.0	36.0
10	34.80820004347902	36.0	36.0	36.0	32.0	36.0
11	35.00210273543016	36.0	36.0	36.0	36.0	36.0
12	34.901381333506585	36.0	36.0	36.0	32.0	36.0
13	34.95404717223939	36.0	36.0	36.0	36.0	36.0
14	34.89909368729588	36.0	36.0	36.0	32.0	36.0
15	34.86814112190868	36.0	36.0	36.0	32.0	36.0
16	34.87890717728696	36.0	36.0	36.0	32.0	36.0
17	34.83631023031387	36.0	36.0	36.0	32.0	36.0
18	34.83529197162369	36.0	36.0	36.0	32.0	36.0
19	34.82641750355454	36.0	36.0	36.0	32.0	36.0
20	34.81261791185753	36.0	36.0	36.0	32.0	36.0
21	34.797791315690176	36.0	36.0	36.0	32.0	36.0
22	34.78315212909771	36.0	36.0	36.0	32.0	36.0
23	34.71888813647415	36.0	36.0	36.0	32.0	36.0
24	34.713127166142336	36.0	36.0	36.0	32.0	36.0
25	34.692855697126134	36.0	36.0	36.0	32.0	36.0
26	34.61541056440267	36.0	36.0	36.0	32.0	36.0
27	34.601398575187474	36.0	36.0	36.0	32.0	36.0
28	34.56386543492765	36.0	36.0	36.0	32.0	36.0
29	34.525029173423825	36.0	36.0	36.0	32.0	36.0
30	34.51464668297547	36.0	36.0	36.0	32.0	36.0
31	34.482557166167325	36.0	36.0	36.0	32.0	36.0
32	34.44808379956671	36.0	36.0	36.0	32.0	36.0
33	34.4141264339956	36.0	36.0	36.0	32.0	36.0
34	34.38092870190133	36.0	36.0	36.0	32.0	36.0
35	34.33551686311355	36.0	36.0	36.0	32.0	36.0
36	34.311479711039425	36.0	36.0	36.0	32.0	36.0
37	34.29324725819792	36.0	36.0	36.0	32.0	36.0
38	33.866936703040786	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	9.0
21	18.0
22	62.0
23	192.0
24	527.0
25	1347.0
26	2888.0
27	5630.0
28	9581.0
29	15567.0
30	23299.0
31	33348.0
32	48238.0
33	76618.0
34	181771.0
35	401288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13018161825804	18.42572519227045	12.305433553457744	26.138659636013774
2	15.120454380761284	21.55747351902707	37.438560894368464	25.883511205843178
3	17.592011854280308	26.019570557206144	29.299987755907775	27.08842983260577
4	11.808317247324725	16.934475283769686	36.67235143087389	34.584856038031695
5	13.574325388000291	37.70593188786411	33.533445112733105	15.1862976114025
6	32.59631302435702	36.80016492063194	17.323413107442043	13.280108947568984
7	28.708952361437866	31.469137823111744	21.68567219861541	18.136237616834983
8	27.595917898177348	33.17020085660168	20.075914699664658	19.157966545556317
9	27.627335711474426	14.14914817984744	18.6001037052771	39.62341240340103
10	15.206258019618158	27.560124490711296	32.5243852615192	24.709232228151343
11	36.639370070869525	21.533490599042413	22.685745129594277	19.14139420049379
12	24.238831256614038	24.392258187038422	29.6228920976385	21.74601845870904
13	29.873123559287095	20.092955265278583	25.321189177708227	24.712731997726095
14	23.56650416174196	19.869662887656705	26.207354951236027	30.356477999365307
15	24.754930745915097	27.07693537867979	23.725302541523714	24.4428313338814
16	24.73943822105834	26.22147313921033	24.753431469316055	24.285657170415277
17	23.332741952008156	26.008325982713345	26.21260241933267	24.44632964594583
18	24.32776185490501	24.840889270926777	27.470620425644626	23.360728448523588
19	24.77741989490071	25.561916375348893	26.604538310265298	23.0561254194851
20	25.11888014033229	24.247675496572903	26.695869243090208	23.9375751200046
21	26.268950231513294	24.62499344066488	25.46483821556099	23.641218112260834
22	24.979447416621483	24.783292061580287	26.01107465647825	24.226185865319984
23	23.762689205819697	24.464601410571163	26.507243389118983	25.26546599449015
24	23.946820659032017	25.60639491445378	26.318676238714822	24.128108187799388
25	24.375238947207972	24.613748866172074	26.338166834502353	24.672845352117605
26	23.929204158993286	25.34751982168604	26.719857668674862	24.003418350645813
27	24.71782365008883	25.197092402915594	26.00107947915131	24.084004467844263
28	23.938574637737293	24.87962058306867	26.700991771470267	24.48081300772377
29	23.691693757761882	25.39149860192457	26.785076200733148	24.131731439580403
30	24.0384014712901	25.321907179785754	26.818560044778394	23.821131304145748
31	24.154720347432367	25.200215895830265	26.017196702591	24.627867054146375
32	23.843620453131365	25.480455680134334	26.02331874870375	24.65260511803055
33	23.696816286141935	24.912729607964156	26.50233762209734	24.88811648379657
34	24.068886762137268	25.304540559180193	26.729602966568635	23.896969712113904
35	25.030672700422045	25.10851014385559	26.314553228067457	23.546263927654906
36	24.066637847238706	25.825664117063518	26.064298975744205	24.043399059953572
37	24.709952447943866	25.669989230196432	25.907874450577594	23.712183871282104
38	23.906839949724258	25.458091470865305	26.24995939459211	24.385109184818322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	342.0
1	264.5
2	187.0
3	187.0
4	652.5
5	1118.0
6	1118.0
7	1283.0
8	1448.0
9	1408.0
10	1368.0
11	1368.0
12	1729.0
13	2090.0
14	2544.0
15	2998.0
16	2998.0
17	4598.5
18	6199.0
19	6199.0
20	6931.0
21	7663.0
22	7740.0
23	7817.0
24	7817.0
25	8792.0
26	9767.0
27	9767.0
28	12198.5
29	14630.0
30	18016.0
31	21402.0
32	21402.0
33	25617.0
34	29832.0
35	29832.0
36	32843.5
37	35855.0
38	40733.0
39	45611.0
40	45611.0
41	49371.0
42	53131.0
43	58925.5
44	64720.0
45	64720.0
46	69367.0
47	74014.0
48	74014.0
49	75379.5
50	76745.0
51	73856.5
52	70968.0
53	70968.0
54	67331.0
55	63694.0
56	63694.0
57	61059.5
58	58425.0
59	52184.0
60	45943.0
61	45943.0
62	42469.5
63	38996.0
64	31966.0
65	24936.0
66	24936.0
67	20714.0
68	16492.0
69	16492.0
70	13276.0
71	10060.0
72	7830.5
73	5601.0
74	5601.0
75	4182.5
76	2764.0
77	2764.0
78	2616.0
79	2468.0
80	1894.5
81	1321.0
82	1321.0
83	1251.5
84	1182.0
85	1182.0
86	743.5
87	305.0
88	258.5
89	212.0
90	212.0
91	130.5
92	49.0
93	34.0
94	19.0
95	19.0
96	14.5
97	10.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0101201170435265
2	0.0
3	0.0
4	1.2493971658674688E-4
5	0.0
6	1.2493971658674688E-4
7	6.246985829337345E-4
8	0.0017491560322144563
9	0.005122528380056623
10	6.246985829337345E-4
11	0.0052474680966433694
12	6.246985829337345E-4
13	1.2493971658674688E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.2493971658674688E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.69799728020189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97739129464627	52.85493645004627
2	11.583804813327983	14.757303349875109
3	2.7388110657333624	5.233703394482117
4	0.9980067088503337	2.5428411450388717
5	0.46820611756182284	1.491189600151344
6	0.2651597766291149	1.0134088038324183
7	0.16303827075205796	0.7269647928853384
8	0.1144597773240603	0.5832686867744405
9	0.08965061566197191	0.5139508205344241
>10	0.46035564707857846	5.584164409442368
>50	0.07144694692715758	3.2227895241478755
>100	0.06315600273250212	7.74573776763301
>500	0.005328787724843514	2.0745648575094893
>1k	0.0011841750499652255	1.6551763976469482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4048	0.5057559727431514	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2981	0.3724452951450925	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1777	0.22201787637464923	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1599	0.1997786068222083	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1496	0.18690981601377335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1265	0.1580487414822348	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	859	0.10732321654801558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2493971658674688E-4
13	0.0	0.0	0.0	0.0	1.2493971658674688E-4
14	0.0	0.0	0.0	0.0	1.2493971658674688E-4
15	0.0	0.0	0.0	0.0	1.2493971658674688E-4
16	0.0	0.0	0.0	0.0	1.2493971658674688E-4
17	0.0	0.0	0.0	0.0	1.2493971658674688E-4
18	0.0	0.0	0.0	1.2493971658674688E-4	1.2493971658674688E-4
19	0.0	0.0	0.0	1.2493971658674688E-4	1.2493971658674688E-4
20	0.0	0.0	0.0	1.2493971658674688E-4	1.2493971658674688E-4
21	0.0	0.0	0.0	9.99517732693975E-4	1.2493971658674688E-4
22	0.0	0.0	0.0	0.0018740957488012034	1.2493971658674688E-4
23	0.0	0.0	0.0	0.002748673764908432	1.2493971658674688E-4
24	0.0	0.0	0.0	0.004372890080536141	1.2493971658674688E-4
25	0.0	0.0	0.0	0.005497347529816864	1.2493971658674688E-4
26	0.0	0.0	0.0	0.006621804979097585	1.2493971658674688E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGT	45	3.533235E-4	21.335335	1
TCTAGCG	195	0.0	18.871206	28
CTAGCGG	195	0.0	18.871206	29
TAGCGGC	205	0.0	17.95066	30
CCCTATA	45	0.008849584	17.777222	2
TCTATTC	55	0.0013632987	17.45618	3
TCTTATG	85	4.981599E-6	16.940647	2
GCATTCG	225	0.0	16.355045	22
CTATACT	70	3.6920098E-4	16.0015	4
CTTATAC	110	2.0147672E-7	16.0015	3
ATAACAC	60	0.0024395103	16.0015	3
CGCCGGT	200	0.0	16.0015	7
CCCGCCG	140	7.403287E-10	16.0015	8
CGTATTG	230	0.0	15.999499	27
CGGCGGG	135	7.899871E-9	15.406926	31
GGCGGGT	135	7.899871E-9	15.406926	32
TATTGCG	240	0.0	15.332853	29
CGGTCCA	240	0.0	15.332853	10
TACCGTC	115	3.3375E-7	15.305782	7
TCTTGCG	230	0.0	15.303869	2
>>END_MODULE