FastQCFastQC Report
Thu 2 Feb 2017
SRR4063022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585877
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30140.5144424512312311No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23980.4093009283518554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21210.36202137991421407No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19900.3396617378733079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11310.19304393242950313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.18024261065035835No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9280.15839502148061796No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9160.15634680999595477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8630.14730054260535913No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7640.13040279785688805No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG7570.12920800782416786No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7280.12425816340289855No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG7170.122380636208624No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7110.12135653046629243No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7060.12050310901434944No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6990.11930831898162925No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6950.11862558182007486No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6510.1111154730429766No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC6380.10889657726792484No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA6180.10548289146015288No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG6120.10445878571782131No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG5930.10121578420043797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTG453.5345485E-421.33276410
ATCGTTT1001.4551915E-1020.79944429
TAATCCC400.00448380819.9977595
CATCGTT1052.7830538E-1019.80899428
ATATTAG1003.254172E-919.19948810
TAACCTA507.1955984E-419.197855
CCAACGA1351.8189894E-1218.96245829
TATACAC2200.018.9069733
TCCAACG1800.018.66616828
CGAGCCG1900.018.52582215
GATATAC2000.018.4168011
CGTTATT701.7813034E-518.2899112
AACGAAT1401.8189894E-1218.28522731
GTTCAAA2300.018.103471
TAGAACA2750.018.0343444
GTTATTC802.7944243E-617.9979843
AATCCCG1251.4733814E-1017.91952119
CCCGTTT1251.4733814E-1017.91952122
TATATAG450.00883355817.7818552
TACGCTA1800.017.7773049
Produced by FastQC (version 0.11.5)