##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063022_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585877
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.090061565823543	32.0	32.0	32.0	32.0	32.0
2	30.710845450495583	32.0	32.0	32.0	32.0	32.0
3	30.792338664941617	32.0	32.0	32.0	32.0	32.0
4	30.830810903995207	32.0	32.0	32.0	32.0	32.0
5	30.78124077238055	32.0	32.0	32.0	32.0	32.0
6	34.39848807855574	36.0	36.0	36.0	32.0	36.0
7	34.28385309544495	36.0	36.0	36.0	32.0	36.0
8	34.277706754148056	36.0	36.0	36.0	32.0	36.0
9	34.38839380962216	36.0	36.0	36.0	32.0	36.0
10	34.131029209202616	36.0	36.0	36.0	32.0	36.0
11	34.438518665180574	36.0	36.0	36.0	32.0	36.0
12	34.233472213450945	36.0	36.0	36.0	32.0	36.0
13	34.314311707064796	36.0	36.0	36.0	32.0	36.0
14	34.21164852008186	36.0	36.0	36.0	32.0	36.0
15	34.15880466377755	36.0	36.0	36.0	32.0	36.0
16	34.17856990460455	36.0	36.0	36.0	32.0	36.0
17	34.0959706559568	36.0	36.0	36.0	32.0	36.0
18	34.13987406913055	36.0	36.0	36.0	32.0	36.0
19	34.12380072950466	36.0	36.0	36.0	32.0	36.0
20	34.08767539944391	36.0	36.0	36.0	32.0	36.0
21	34.05202798539625	36.0	36.0	36.0	32.0	36.0
22	34.03469499570729	36.0	36.0	36.0	32.0	36.0
23	33.97704296294273	36.0	36.0	36.0	32.0	36.0
24	33.97206239534919	36.0	36.0	36.0	32.0	36.0
25	33.93897353881446	36.0	36.0	36.0	32.0	36.0
26	33.89700909917952	36.0	36.0	36.0	32.0	36.0
27	33.90243685961388	36.0	36.0	36.0	32.0	36.0
28	33.86440327918659	36.0	36.0	36.0	32.0	36.0
29	33.84064231912159	36.0	36.0	36.0	32.0	36.0
30	33.790927105860106	36.0	36.0	36.0	32.0	36.0
31	33.8014224828761	36.0	36.0	36.0	32.0	36.0
32	33.764138206483615	36.0	36.0	36.0	32.0	36.0
33	33.683894401043226	36.0	36.0	36.0	27.0	36.0
34	33.68774333179149	36.0	36.0	36.0	27.0	36.0
35	33.62502368244529	36.0	36.0	36.0	27.0	36.0
36	33.579592644872555	36.0	36.0	36.0	27.0	36.0
37	33.56983462399104	36.0	36.0	36.0	27.0	36.0
38	32.91799131899699	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	2.0
10	7.0
11	4.0
12	2.0
13	6.0
14	169.0
15	319.0
16	345.0
17	368.0
18	458.0
19	591.0
20	719.0
21	981.0
22	1538.0
23	2218.0
24	3157.0
25	4558.0
26	6436.0
27	9226.0
28	12650.0
29	16904.0
30	22545.0
31	29810.0
32	40442.0
33	59664.0
34	126555.0
35	246200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.09717206691881	17.295390573328525	11.721386980946017	24.88605037880665
2	15.78723556867145	20.72157923589657	38.2704094789676	25.220775716464377
3	18.80653525819937	25.853736810929195	28.65756127871466	26.68216665215677
4	12.120431091280883	15.924227889844367	36.10713785840352	35.848203160471236
5	13.866268401962875	37.49861318540644	33.34158310219757	15.293535310433112
6	32.75129890966689	36.12351419071612	17.562077982371697	13.563108917245287
7	28.92420921833289	31.439929268309335	21.345472420785285	18.29038909257249
8	28.161686642520404	32.71129969925274	20.0973246807334	19.029688977493453
9	27.756944740772347	14.485001066780455	18.791039044164712	38.967015148282485
10	16.018345304093724	27.109846519376248	31.511149192997788	25.360658983532243
11	36.925912966297695	21.57152244971111	22.301554112295495	19.2010104716957
12	24.74170494366411	24.390768458729973	29.20812274583903	21.659403851766886
13	29.8923666532943	19.79753663886921	25.632100514693956	24.677996193142533
14	23.94084179584479	20.3388760009491	25.542872457192413	30.1774097460137
15	25.02881005509336	27.27283591670608	23.930015894614098	23.768338133586465
16	25.01741184825062	26.08107093109005	25.0833037445716	23.81821347608773
17	23.19243632489795	25.871750090055944	26.431035925066627	24.504777659979478
18	24.396459754026303	24.61085459216332	28.052779366203794	22.93990628760658
19	24.966286917958573	25.667006926971492	26.425935345990105	22.94077080907983
20	25.04075337847641	24.262043796866386	26.840729822596916	23.856473002060284
21	26.785174766446072	24.614441851771563	24.709348472873945	23.89103490890842
22	25.236800374169345	24.90906080422511	25.70758461057882	24.14655421102673
23	23.595302311290155	24.696493803557406	26.10085009047148	25.60735379468096
24	23.779624878154536	26.350201354426467	26.0048516259434	23.8653221414756
25	24.617511992761663	24.73991498369667	25.98067500896256	24.6618980145791
26	23.297095758701456	25.996647113854728	26.977588243729556	23.72866888371426
27	24.699311394928834	25.44322336819034	26.029066849657408	23.828398387223423
28	23.564805647919922	25.276012044161927	26.749953912645687	24.40922839527246
29	23.271645928750793	25.546130695814124	27.004812197957335	24.177411177477754
30	23.787504821987582	25.73934298072229	26.970699384492487	23.502452812797642
31	23.8649999914652	25.563083246705144	25.751190177472573	24.820726584357082
32	23.317959292361685	26.042100519599344	25.835045985565923	24.804894202473047
33	23.110393412344685	25.42713413437164	26.497423283141835	24.965049170141835
34	23.749743939911234	26.10139979515193	26.758962103106864	23.38989416182998
35	24.943537497418003	25.5290745612313	26.45159418013715	23.075793761213546
36	23.60754750479273	26.50776823499487	25.73461503662619	24.15006922358621
37	24.595864244526457	26.39727696998044	25.81467281647201	23.192185969021097
38	23.738271175041778	26.313875901905458	25.818521344455768	24.129331578596997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1004.0
1	687.0
2	370.0
3	370.0
4	763.0
5	1156.0
6	1156.0
7	1273.0
8	1390.0
9	1307.0
10	1224.0
11	1224.0
12	1546.5
13	1869.0
14	2233.0
15	2597.0
16	2597.0
17	3578.0
18	4559.0
19	4559.0
20	5203.5
21	5848.0
22	5765.0
23	5682.0
24	5682.0
25	6549.5
26	7417.0
27	7417.0
28	9568.0
29	11719.0
30	14060.5
31	16402.0
32	16402.0
33	19506.0
34	22610.0
35	22610.0
36	24315.5
37	26021.0
38	29533.5
39	33046.0
40	33046.0
41	34361.5
42	35677.0
43	40480.0
44	45283.0
45	45283.0
46	49666.0
47	54049.0
48	54049.0
49	54113.0
50	54177.0
51	52120.0
52	50063.0
53	50063.0
54	47247.0
55	44431.0
56	44431.0
57	43177.5
58	41924.0
59	37749.5
60	33575.0
61	33575.0
62	31951.5
63	30328.0
64	24929.5
65	19531.0
66	19531.0
67	16336.0
68	13141.0
69	13141.0
70	10602.5
71	8064.0
72	6207.0
73	4350.0
74	4350.0
75	3291.0
76	2232.0
77	2232.0
78	2206.0
79	2180.0
80	1703.5
81	1227.0
82	1227.0
83	1306.5
84	1386.0
85	1386.0
86	887.5
87	389.0
88	351.0
89	313.0
90	313.0
91	231.5
92	150.0
93	141.5
94	133.0
95	133.0
96	118.0
97	103.0
98	103.0
99	180.0
100	257.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1280132177914477
2	0.055984447247459795
3	0.011947900327201784
4	0.002560264355828954
5	3.4136858077719385E-4
6	1.7068429038859692E-4
7	1.7068429038859692E-4
8	5.120528711657907E-4
9	3.4136858077719385E-4
10	8.534214519429847E-4
11	0.0020482114846631628
12	0.002730948646217551
13	0.01570295471575092
14	0.009899688842538622
15	0.02457853781595796
16	0.011777216036813188
17	0.022188957750517598
18	0.00716874019632107
19	0.008704898809818444
20	0.00614463445398949
21	0.006486003034766683
22	0.00785147735787546
23	0.009729004552150025
24	0.016044323296528114
25	0.01826321907157987
26	0.01928732481391145
27	0.009387635971372831
28	0.005632581582823698
29	0.012630637488756171
30	0.0032430015173833416
31	0.006827371615543878
32	0.006998055905932474
33	0.009216951680984234
34	0.013142690359921964
35	0.015361586134973723
36	0.016044323296528114
37	0.010070373132927218
38	0.005803265873212296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	585877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16062095885786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09466203988737	46.10487197567652
2	11.52223705642983	12.941919758485238
3	3.0522496233915017	5.142487025133204
4	1.1196506123568069	2.5152109458769494
5	0.547407480701571	1.5371372016862115
6	0.30038005072676227	1.0121718103480917
7	0.19708588768030094	0.7747926084047391
8	0.13429740201442594	0.6033780392233224
9	0.11401948371162977	0.5763064505988158
>10	0.687951718843004	7.851859729770312
>50	0.11319861877968243	4.53339224421171
>100	0.10770649867740643	11.679447902253525
>500	0.007322821439823618	2.7204465279152616
>1k	0.0018307053599559045	2.0065777804162073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3014	0.5144424512312311	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2398	0.4093009283518554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2121	0.36202137991421407	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1990	0.3396617378733079	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1131	0.19304393242950313	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.18024261065035835	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	928	0.15839502148061796	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	916	0.15634680999595477	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.14730054260535913	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	764	0.13040279785688805	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	757	0.12920800782416786	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	728	0.12425816340289855	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	717	0.122380636208624	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	711	0.12135653046629243	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	706	0.12050310901434944	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	699	0.11930831898162925	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	695	0.11862558182007486	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	651	0.1111154730429766	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	638	0.10889657726792484	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	618	0.10548289146015288	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	612	0.10445878571782131	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	593	0.10121578420043797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.7068429038859692E-4	0.0
6	0.0	0.0	0.0	1.7068429038859692E-4	0.0
7	0.0	0.0	0.0	1.7068429038859692E-4	0.0
8	0.0	0.0	0.0	1.7068429038859692E-4	0.0
9	0.0	0.0	0.0	3.4136858077719385E-4	0.0
10	0.0	0.0	0.0	3.4136858077719385E-4	0.0
11	0.0	0.0	0.0	3.4136858077719385E-4	0.0
12	0.0	0.0	0.0	3.4136858077719385E-4	1.7068429038859692E-4
13	0.0	0.0	0.0	3.4136858077719385E-4	1.7068429038859692E-4
14	0.0	0.0	0.0	3.4136858077719385E-4	1.7068429038859692E-4
15	0.0	0.0	0.0	3.4136858077719385E-4	1.7068429038859692E-4
16	0.0	0.0	0.0	3.4136858077719385E-4	5.120528711657908E-4
17	0.0	0.0	0.0	5.120528711657908E-4	5.120528711657908E-4
18	0.0	0.0	0.0	6.827371615543877E-4	6.827371615543877E-4
19	0.0	0.0	0.0	8.534214519429846E-4	6.827371615543877E-4
20	0.0	0.0	0.0	0.0011947900327201784	6.827371615543877E-4
21	0.0	0.0	0.0	0.0011947900327201784	6.827371615543877E-4
22	0.0	0.0	0.0	0.0015361586134973724	6.827371615543877E-4
23	0.0	0.0	0.0	0.0037550543885491324	8.534214519429846E-4
24	0.0	0.0	0.0	0.005803265873212295	8.534214519429846E-4
25	0.0	0.0	0.0	0.00665668732515528	8.534214519429846E-4
26	0.0	0.0	0.0	0.007680793067486862	8.534214519429846E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCTG	45	3.5345485E-4	21.332764	10
ATCGTTT	100	1.4551915E-10	20.799444	29
TAATCCC	40	0.004483808	19.997759	5
CATCGTT	105	2.7830538E-10	19.808994	28
ATATTAG	100	3.254172E-9	19.199488	10
TAACCTA	50	7.1955984E-4	19.19785	5
CCAACGA	135	1.8189894E-12	18.962458	29
TATACAC	220	0.0	18.906973	3
TCCAACG	180	0.0	18.666168	28
CGAGCCG	190	0.0	18.525822	15
GATATAC	200	0.0	18.416801	1
CGTTATT	70	1.7813034E-5	18.289911	2
AACGAAT	140	1.8189894E-12	18.285227	31
GTTCAAA	230	0.0	18.10347	1
TAGAACA	275	0.0	18.034344	4
GTTATTC	80	2.7944243E-6	17.997984	3
AATCCCG	125	1.4733814E-10	17.919521	19
CCCGTTT	125	1.4733814E-10	17.919521	22
TATATAG	45	0.008833558	17.781855	2
TACGCTA	180	0.0	17.777304	9
>>END_MODULE