FastQCFastQC Report
Thu 2 Feb 2017
SRR4063022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585877
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38970.6651566796443622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29770.508127132486853No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24410.4166403528385651No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23510.40127876670359136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17670.30159914111665076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.23895800654403568No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9670.16505170880577325No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8010.13671811660126615No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7840.13381648366465998No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7730.13193895647038542No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7540.12869595495300207No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7530.12852527066261346No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7440.12698911204911612No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7310.12477021627406434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.1220392676278468No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG7130.1216978990470696No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7070.12067379330473803No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7060.12050310901434944No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG6940.11845489752968627No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6810.11623600175463451No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.11589463317385731No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG6270.10701905007365027No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6190.10565357575054149No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6060.10343467997548973No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5920.10104509991004937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1250.021.75953529
GTCGTAA453.534421E-421.33287630
TAGCGGC1250.020.47956330
ACTAGTA751.5050664E-619.201238
GTTTTCG2250.019.1995928
ACCGTCG1351.8189894E-1218.9641768
TCTAGCG1450.018.7582228
CAAGACG1800.018.6662674
GATATAC2250.018.4900721
TTTCGGA2350.018.38258630
AACCGCG1055.9553713E-918.2868847
CCGTCGT1401.8189894E-1218.2868849
CTATAGA904.3547698E-717.7789151
CCATGCG450.0088419817.7789159
GGGCGTC450.0088419817.7789157
CGTCGTA1352.3646862E-1117.77891510
ATTAGTG450.008846329517.7773973
CAAGCGA450.008846329517.77739726
CCTATCC450.008846329517.7773973
TATACAC2250.017.7773973
Produced by FastQC (version 0.11.5)