##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585877
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12268274740261	32.0	32.0	32.0	32.0	32.0
2	31.314065921686634	32.0	32.0	32.0	32.0	32.0
3	31.386164672789683	32.0	32.0	32.0	32.0	32.0
4	31.487866907217725	32.0	32.0	32.0	32.0	32.0
5	31.413192530172715	32.0	32.0	32.0	32.0	32.0
6	34.92637362449798	36.0	36.0	36.0	36.0	36.0
7	34.951520541000924	36.0	36.0	36.0	36.0	36.0
8	34.904060067215475	36.0	36.0	36.0	36.0	36.0
9	34.99365907861206	36.0	36.0	36.0	36.0	36.0
10	34.85965654907088	36.0	36.0	36.0	32.0	36.0
11	35.01880599511502	36.0	36.0	36.0	36.0	36.0
12	34.92718949540603	36.0	36.0	36.0	32.0	36.0
13	34.96701867456821	36.0	36.0	36.0	36.0	36.0
14	34.92126675052955	36.0	36.0	36.0	32.0	36.0
15	34.87631021528409	36.0	36.0	36.0	32.0	36.0
16	34.899366249229786	36.0	36.0	36.0	32.0	36.0
17	34.86082232277423	36.0	36.0	36.0	32.0	36.0
18	34.85544406078409	36.0	36.0	36.0	32.0	36.0
19	34.84717952744347	36.0	36.0	36.0	32.0	36.0
20	34.821766343447514	36.0	36.0	36.0	32.0	36.0
21	34.80053833825188	36.0	36.0	36.0	32.0	36.0
22	34.794400531169515	36.0	36.0	36.0	32.0	36.0
23	34.71565704064164	36.0	36.0	36.0	32.0	36.0
24	34.708003557060614	36.0	36.0	36.0	32.0	36.0
25	34.687043526200895	36.0	36.0	36.0	32.0	36.0
26	34.62329465058365	36.0	36.0	36.0	32.0	36.0
27	34.593303713919475	36.0	36.0	36.0	32.0	36.0
28	34.554071929773656	36.0	36.0	36.0	32.0	36.0
29	34.505624900789755	36.0	36.0	36.0	32.0	36.0
30	34.47787334201547	36.0	36.0	36.0	32.0	36.0
31	34.468414018642136	36.0	36.0	36.0	32.0	36.0
32	34.41248931089631	36.0	36.0	36.0	32.0	36.0
33	34.36379820337716	36.0	36.0	36.0	32.0	36.0
34	34.32718130256009	36.0	36.0	36.0	32.0	36.0
35	34.270749662471815	36.0	36.0	36.0	32.0	36.0
36	34.21625528907945	36.0	36.0	36.0	32.0	36.0
37	34.20374071690816	36.0	36.0	36.0	32.0	36.0
38	33.75213227349768	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	17.0
22	41.0
23	133.0
24	387.0
25	977.0
26	2129.0
27	4220.0
28	7293.0
29	11443.0
30	17194.0
31	24751.0
32	34941.0
33	55168.0
34	131094.0
35	296078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6894015950183	18.454348063582238	12.40799284426722	25.448257497132243
2	14.948043613324321	21.634782786801303	37.481310038301615	25.93586356157276
3	17.931067442483663	26.338804902735557	29.024863580580906	26.705264074199874
4	11.721080911319119	16.661716813796776	36.070260601219374	35.54694167366473
5	13.419028226061103	37.85504465954458	33.15815435663117	15.567772757763148
6	32.9994742914883	36.320484198021425	17.109763840812732	13.570277669677544
7	28.546703648389162	31.51525496052912	21.538553445700874	18.39948794538084
8	27.960755397157016	32.84021547662939	20.095448773094095	19.103580353119494
9	27.64095115036212	14.026583188104988	18.788991890540217	39.54347377099268
10	15.829881219991362	27.183707049138633	31.457500174952592	25.52891155591741
11	36.99858665446774	21.459979107066047	22.296208546985206	19.245225691481007
12	24.485556670694823	24.3990195877626	29.38932470352088	21.726099038021697
13	30.017563413480985	19.738784761989294	25.553145114076848	24.690506710452876
14	23.864736113552844	20.14672021601804	25.728437880305933	30.26010579012318
15	25.004565804767896	27.157577443729657	23.953662628845304	23.884194122657146
16	24.90300865198668	25.770084847160753	25.241987652698434	24.084918848154135
17	23.128916137687604	25.883931268849945	26.485422708179364	24.501729885283087
18	24.46503276284954	24.207811537233926	28.41773955966867	22.909416140247867
19	24.90778781211756	25.34115522541421	26.742131880923814	23.008925081544422
20	24.923149398252534	23.938471727000717	27.42384493673587	23.714533938010877
21	26.634816923717647	24.324089056387358	25.299551440919238	23.741542578975753
22	24.947386567487715	24.709281982395623	26.27155529232245	24.071776157794215
23	23.35148845235433	24.251336031283017	26.788387323619123	25.608788192743525
24	23.841693734350383	25.839382669058526	26.423805679349076	23.895117917242015
25	24.58929092625244	24.23017117927483	26.480643548048484	24.69989434642425
26	23.47096745562635	25.36744060613405	27.475562276723615	23.686029661515985
27	24.716322225180757	25.08534229086018	26.417535451187625	23.78080003277144
28	23.472844982820625	24.796330970493806	27.343964688834006	24.386859357851563
29	23.350293662321615	24.935609351450903	27.56192852766024	24.152168458567242
30	23.928913406738957	24.984937111373206	27.598625650093787	23.487523831794046
31	23.818822039438313	24.827224827054142	26.36048180761491	24.993471325892635
32	23.425565434382985	25.533687104972547	26.307398993304055	24.733348467340416
33	23.194117536616048	24.86631152955313	26.951049452359456	24.988521481471366
34	23.88351138549559	25.31026136885387	27.309315777885118	23.49691146776542
35	25.141454605659554	25.07113267801945	26.820475970212176	22.966936746108825
36	23.654282383503705	25.92882123722215	26.394277297111852	24.022619082162297
37	24.671560754219744	25.77588811303396	26.299718200236565	23.252832932509726
38	23.880609752558986	25.586769919283398	26.265752026449235	24.26686830170838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	441.0
1	339.5
2	238.0
3	238.0
4	755.5
5	1273.0
6	1273.0
7	1475.0
8	1677.0
9	1511.0
10	1345.0
11	1345.0
12	1643.5
13	1942.0
14	2407.5
15	2873.0
16	2873.0
17	4111.5
18	5350.0
19	5350.0
20	5880.0
21	6410.0
22	6149.5
23	5889.0
24	5889.0
25	6578.5
26	7268.0
27	7268.0
28	9283.5
29	11299.0
30	13673.5
31	16048.0
32	16048.0
33	19137.5
34	22227.0
35	22227.0
36	23956.0
37	25685.0
38	29231.0
39	32777.0
40	32777.0
41	33890.0
42	35003.0
43	39604.5
44	44206.0
45	44206.0
46	49382.5
47	54559.0
48	54559.0
49	55115.0
50	55671.0
51	53085.5
52	50500.0
53	50500.0
54	47520.0
55	44540.0
56	44540.0
57	43381.5
58	42223.0
59	37918.5
60	33614.0
61	33614.0
62	31983.5
63	30353.0
64	25008.5
65	19664.0
66	19664.0
67	16398.5
68	13133.0
69	13133.0
70	10573.0
71	8013.0
72	6188.0
73	4363.0
74	4363.0
75	3251.0
76	2139.0
77	2139.0
78	2181.0
79	2223.0
80	1722.5
81	1222.0
82	1222.0
83	1192.0
84	1162.0
85	1162.0
86	722.5
87	283.0
88	245.0
89	207.0
90	207.0
91	118.0
92	29.0
93	22.5
94	16.0
95	16.0
96	12.0
97	8.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009046267390595636
2	1.7068429038859692E-4
3	0.0
4	1.7068429038859692E-4
5	0.0
6	1.7068429038859692E-4
7	3.4136858077719385E-4
8	0.00221889577505176
9	0.003072317226994745
10	6.827371615543877E-4
11	0.005632581582823698
12	1.7068429038859692E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.7068429038859692E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.7068429038859692E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	585877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.8864543997477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8045348887567	43.542698845758096
2	12.325862993684051	13.283941082933865
3	3.2703745593288445	5.286866686841062
4	1.2231612419420839	2.6364728994980346
5	0.5969128044734907	1.6082757309443134
6	0.3279910801198645	1.060456582944184
7	0.22060524564413753	0.8321344156823566
8	0.15799245364383133	0.6810922519026055
9	0.09628669470577318	0.4669693725208567
>10	0.7263328893245534	7.909063225125998
>50	0.12385817020214357	4.800847347393066
>100	0.11398772269315192	11.787048619858254
>500	0.010188846721175574	3.5591571430492355
>1k	0.00191040876022042	2.5449757955480767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3897	0.6651566796443622	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2977	0.508127132486853	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2441	0.4166403528385651	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2351	0.40127876670359136	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1767	0.30159914111665076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1400	0.23895800654403568	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	967	0.16505170880577325	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	801	0.13671811660126615	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	784	0.13381648366465998	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	773	0.13193895647038542	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	754	0.12869595495300207	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	753	0.12852527066261346	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	744	0.12698911204911612	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	731	0.12477021627406434	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	715	0.1220392676278468	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	713	0.1216978990470696	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	707	0.12067379330473803	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	706	0.12050310901434944	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	694	0.11845489752968627	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	681	0.11623600175463451	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.11589463317385731	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	627	0.10701905007365027	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	619	0.10565357575054149	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	606	0.10343467997548973	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	592	0.10104509991004937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.7068429038859692E-4	0.0
6	0.0	0.0	0.0	1.7068429038859692E-4	0.0
7	0.0	0.0	0.0	1.7068429038859692E-4	0.0
8	0.0	0.0	0.0	1.7068429038859692E-4	0.0
9	0.0	0.0	0.0	1.7068429038859692E-4	0.0
10	0.0	0.0	0.0	1.7068429038859692E-4	0.0
11	0.0	0.0	0.0	1.7068429038859692E-4	0.0
12	0.0	0.0	0.0	1.7068429038859692E-4	5.120528711657908E-4
13	0.0	0.0	0.0	1.7068429038859692E-4	5.120528711657908E-4
14	0.0	0.0	0.0	1.7068429038859692E-4	5.120528711657908E-4
15	0.0	0.0	0.0	1.7068429038859692E-4	8.534214519429846E-4
16	0.0	0.0	0.0	1.7068429038859692E-4	0.0010241057423315816
17	0.0	0.0	0.0	3.4136858077719385E-4	0.0010241057423315816
18	0.0	0.0	0.0	3.4136858077719385E-4	0.0011947900327201784
19	0.0	0.0	0.0	5.120528711657908E-4	0.0011947900327201784
20	0.0	0.0	0.0	8.534214519429846E-4	0.0011947900327201784
21	0.0	0.0	0.0	8.534214519429846E-4	0.0011947900327201784
22	0.0	0.0	0.0	0.0013654743231087754	0.0011947900327201784
23	0.0	0.0	0.0	0.0034136858077719384	0.0013654743231087754
24	0.0	0.0	0.0	0.005461897292435102	0.0013654743231087754
25	0.0	0.0	0.0	0.006315318744378086	0.0013654743231087754
26	0.0	0.0	0.0	0.007339424486709668	0.0017068429038859692
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	125	0.0	21.759535	29
GTCGTAA	45	3.534421E-4	21.332876	30
TAGCGGC	125	0.0	20.479563	30
ACTAGTA	75	1.5050664E-6	19.20123	8
GTTTTCG	225	0.0	19.19959	28
ACCGTCG	135	1.8189894E-12	18.964176	8
TCTAGCG	145	0.0	18.75822	28
CAAGACG	180	0.0	18.666267	4
GATATAC	225	0.0	18.490072	1
TTTCGGA	235	0.0	18.382586	30
AACCGCG	105	5.9553713E-9	18.286884	7
CCGTCGT	140	1.8189894E-12	18.286884	9
CTATAGA	90	4.3547698E-7	17.778915	1
CCATGCG	45	0.00884198	17.778915	9
GGGCGTC	45	0.00884198	17.778915	7
CGTCGTA	135	2.3646862E-11	17.778915	10
ATTAGTG	45	0.0088463295	17.777397	3
CAAGCGA	45	0.0088463295	17.777397	26
CCTATCC	45	0.0088463295	17.777397	3
TATACAC	225	0.0	17.777397	3
>>END_MODULE