FastQCFastQC Report
Thu 2 Feb 2017
SRR4063021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796323
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31480.17524687931958785No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26470.1473565722868326No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24600.13694641776562455No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21380.11902091104996151No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21200.11801886409070084No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21080.11735083278452707No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20930.11651579365180983No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20700.11523540031497675No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20580.11456736900880297No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19970.1111715432024196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19540.1087777643552969No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18680.10399020666105149No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17970.1000376880995233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC507.197592E-419.200212
TCTAGCG4400.018.54461528
CTAGCGG4350.018.39099729
TAGCGGC4600.017.39148530
GTATCAA24600.016.8611981
CGAGCCG4800.016.33350415
GTGTTAT1101.9997788E-716.0148811
CGTTTAT3950.015.79763331
CAAGACG6050.015.5991254
AGCGGCG5250.015.5430231
ATACCGT5150.015.53068356
TACCGTC4850.015.5040227
CTTACAC1451.2259989E-915.4449973
ATCGTTT4500.015.28904829
ACACCGT1052.1913274E-615.234866
GTCTTAG2000.015.2141381
CGCCGGT5750.015.0249887
AAGACGG6300.014.9809475
ACCGTCG5050.014.8900018
GCGCAAG6450.014.6492721
Produced by FastQC (version 0.11.5)