##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063021_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1796323
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27222331395857	32.0	32.0	32.0	32.0	32.0
2	30.892910684771056	32.0	32.0	32.0	32.0	32.0
3	30.942476937610888	32.0	32.0	32.0	32.0	32.0
4	31.008942712418648	32.0	32.0	32.0	32.0	32.0
5	30.919078584419395	32.0	32.0	32.0	32.0	32.0
6	34.57715900759496	36.0	36.0	36.0	32.0	36.0
7	34.52130268331475	36.0	36.0	36.0	32.0	36.0
8	34.51000293377082	36.0	36.0	36.0	32.0	36.0
9	34.64831324878655	36.0	36.0	36.0	32.0	36.0
10	34.35860198861786	36.0	36.0	36.0	32.0	36.0
11	34.618021925900855	36.0	36.0	36.0	32.0	36.0
12	34.43482324726678	36.0	36.0	36.0	32.0	36.0
13	34.525980572536234	36.0	36.0	36.0	32.0	36.0
14	34.41972406966899	36.0	36.0	36.0	32.0	36.0
15	34.375723074302336	36.0	36.0	36.0	32.0	36.0
16	34.39460609255685	36.0	36.0	36.0	32.0	36.0
17	34.314425635033345	36.0	36.0	36.0	32.0	36.0
18	34.33544357000383	36.0	36.0	36.0	32.0	36.0
19	34.33411752786108	36.0	36.0	36.0	32.0	36.0
20	34.32346020175659	36.0	36.0	36.0	32.0	36.0
21	34.307318338628406	36.0	36.0	36.0	32.0	36.0
22	34.28961940586409	36.0	36.0	36.0	32.0	36.0
23	34.222685452449255	36.0	36.0	36.0	32.0	36.0
24	34.20738976230889	36.0	36.0	36.0	32.0	36.0
25	34.194659869077	36.0	36.0	36.0	32.0	36.0
26	34.14822779644864	36.0	36.0	36.0	32.0	36.0
27	34.15226771577272	36.0	36.0	36.0	32.0	36.0
28	34.135172237954976	36.0	36.0	36.0	32.0	36.0
29	34.10636171779797	36.0	36.0	36.0	32.0	36.0
30	34.083918649374304	36.0	36.0	36.0	32.0	36.0
31	34.093669122980664	36.0	36.0	36.0	32.0	36.0
32	34.060077725442476	36.0	36.0	36.0	32.0	36.0
33	34.01745120448828	36.0	36.0	36.0	32.0	36.0
34	34.02978194901474	36.0	36.0	36.0	32.0	36.0
35	33.99110906000758	36.0	36.0	36.0	32.0	36.0
36	33.95790122377768	36.0	36.0	36.0	32.0	36.0
37	33.96723473451044	36.0	36.0	36.0	32.0	36.0
38	33.38863444937241	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	20.0
11	25.0
12	8.0
13	9.0
14	161.0
15	354.0
16	525.0
17	603.0
18	781.0
19	1001.0
20	1362.0
21	2077.0
22	3125.0
23	4944.0
24	7321.0
25	11459.0
26	16653.0
27	24194.0
28	33933.0
29	45491.0
30	61499.0
31	82926.0
32	113142.0
33	168915.0
34	381408.0
35	834378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41147971724878	17.10648207499529	11.429162258665414	26.05287594909051
2	17.05725112390624	19.069782727241837	37.05941232138789	26.81355382746403
3	19.031411965598778	22.956919412571356	28.300110405940877	29.71155821588899
4	12.85460904071428	14.792676395694643	35.54953197563401	36.803182587957075
5	15.117097928706347	35.9889707662957	33.378518379551046	15.5154129254469
6	35.24932987811158	34.947768069631444	16.306494128257015	13.496407923999968
7	31.111943676053805	29.89501331330724	20.522367079862587	18.47067593077637
8	28.50518198605036	32.62187840801105	19.134630319002362	19.73830928693623
9	27.377036312511727	14.168944003945839	18.46917286033748	39.984846823204954
10	16.138957578780595	26.08380030707258	31.138918610179267	26.63832350396756
11	38.21692609170063	20.966219074977175	21.719776426837687	19.097078406484513
12	24.877468172900702	23.42701645856854	28.18421304616015	23.511302322370604
13	29.40283998155972	18.851903157341482	25.266526657119886	26.478730203978913
14	23.765066261206616	19.41431362320164	24.550580157882223	32.27003995770952
15	25.322652673264678	26.88365794417711	21.849229408862662	25.94445997369555
16	26.364315612364997	25.544763047978343	23.13387547032407	24.95704586933259
17	24.567538207032644	25.82515330420465	24.573663136665555	25.03364535209715
18	25.630008122671832	24.454642477175515	25.570382478188762	24.34496692196389
19	25.894666048314946	24.704580238095105	25.055158940703375	24.345594772886574
20	25.96736695364032	23.997113932845494	24.48307998592597	25.552439127588215
21	27.201131721895266	23.989353084649213	24.200855026803794	24.608660166651728
22	26.21724180933887	23.87172418093389	24.690678947462185	25.22035506226506
23	24.566543179770864	23.776572230926064	25.39793624334824	26.25894834595483
24	25.077155467361973	24.676164180375928	24.83039159844748	25.416288753814626
25	25.127561531317443	24.228895832730142	24.89515566849815	25.748386967454273
26	25.00657028760042	25.22823397350949	25.242432476374805	24.522763262515284
27	25.829640752067316	24.272938682273214	24.421978110077617	25.475442455581852
28	24.89163269691784	24.29955138775842	25.46328205116785	25.345533864155893
29	24.82922959148166	24.712589602616745	25.323682928526694	25.134497877374905
30	24.711218940274136	24.874335910729922	25.536601945044612	24.87784320395133
31	25.416418690698023	24.69289486913828	24.355684666984743	25.535001773178955
32	25.239298580827054	24.44684211610484	24.27336351949992	26.04049578356819
33	24.74359102838621	24.143531122609755	25.04072590657594	26.0721519424281
34	25.71119013378364	24.358376913650563	24.92020250441371	25.010230448152086
35	26.153668839791738	24.076978626760898	25.183919431234763	24.5854331022126
36	24.809065312274328	24.914909692639277	24.681562087110873	25.594462907975522
37	25.946245083934986	24.6074942432489	24.60003385014186	24.84622682267425
38	24.939149586239456	24.36265733144491	25.12821454976261	25.569978532553023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	92.5
2	126.0
3	126.0
4	358.5
5	591.0
6	591.0
7	763.5
8	936.0
9	901.0
10	866.0
11	866.0
12	1176.0
13	1486.0
14	2190.0
15	2894.0
16	2894.0
17	4258.5
18	5623.0
19	5623.0
20	7178.5
21	8734.0
22	9889.5
23	11045.0
24	11045.0
25	13273.5
26	15502.0
27	15502.0
28	19593.5
29	23685.0
30	29238.0
31	34791.0
32	34791.0
33	45459.5
34	56128.0
35	56128.0
36	63992.0
37	71856.0
38	82885.0
39	93914.0
40	93914.0
41	101535.5
42	109157.0
43	125238.5
44	141320.0
45	141320.0
46	144668.5
47	148017.0
48	148017.0
49	159130.0
50	170243.0
51	173533.5
52	176824.0
53	176824.0
54	168638.0
55	160452.0
56	160452.0
57	154964.5
58	149477.0
59	133776.5
60	118076.0
61	118076.0
62	111507.5
63	104939.0
64	87462.5
65	69986.0
66	69986.0
67	58469.0
68	46952.0
69	46952.0
70	37856.0
71	28760.0
72	22295.0
73	15830.0
74	15830.0
75	11987.0
76	8144.0
77	8144.0
78	7779.5
79	7415.0
80	5798.0
81	4181.0
82	4181.0
83	4087.5
84	3994.0
85	3994.0
86	2696.5
87	1399.0
88	1179.0
89	959.0
90	959.0
91	723.0
92	487.0
93	413.0
94	339.0
95	339.0
96	301.5
97	264.0
98	264.0
99	568.0
100	872.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12453216932589517
2	0.05689399957580012
3	0.012469917715243861
4	0.0027277945002095946
5	3.3401565308688915E-4
6	4.453542041158522E-4
7	0.0
8	2.226771020579261E-4
9	0.0
10	2.7834637757240765E-4
11	0.002393778847122706
12	0.003061810153296484
13	0.014195665256192789
14	0.009686453939519786
15	0.023882119195712575
16	0.012302909888700416
17	0.021377001797560906
18	0.006457635959679857
19	0.008350391327172229
20	0.005956612480049524
21	0.006791651612766746
22	0.007292675092397081
23	0.010076138868121157
24	0.01525338149096794
25	0.01820385309323546
26	0.01998526990969887
27	0.008573068429230156
28	0.006513305235194339
29	0.011022516551867343
30	0.0036741721839557806
31	0.006346297408650894
32	0.007571021469969488
33	0.009408107561947378
34	0.011913224960099047
35	0.016255428450228607
36	0.016144089899199642
37	0.009519446112976341
38	0.006401966684165376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1796323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.891402058347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47960347000992	44.98117875038662
2	11.915457717048096	13.319432759455369
3	3.4557453882491487	5.794393647177347
4	1.4431548194564028	3.2263978498671584
5	0.7212520897157667	2.0155895265863433
6	0.464236750045363	1.5568105708227433
7	0.2917378071410222	1.1413944552177555
8	0.2032633996909605	0.9088541116699165
9	0.15264502749914818	0.7678390143746091
>10	0.7334535116218255	7.246585413485597
>50	0.05843845699643039	2.3022866026521664
>100	0.0646099254162562	7.94954877523589
>500	0.011201118026135715	4.29568301662227
>1k	0.0052005190835630105	4.494005506446289
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3148	0.17524687931958785	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2647	0.1473565722868326	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2460	0.13694641776562455	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2138	0.11902091104996151	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2120	0.11801886409070084	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2108	0.11735083278452707	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2093	0.11651579365180983	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2070	0.11523540031497675	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2058	0.11456736900880297	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1997	0.1111715432024196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1954	0.1087777643552969	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1868	0.10399020666105149	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1797	0.1000376880995233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.566927551448153E-5	0.0
10	0.0	0.0	0.0	5.566927551448153E-5	0.0
11	0.0	0.0	0.0	1.1133855102896306E-4	0.0
12	0.0	0.0	0.0	1.1133855102896306E-4	0.0
13	0.0	0.0	0.0	1.1133855102896306E-4	0.0
14	0.0	0.0	0.0	1.1133855102896306E-4	0.0
15	0.0	0.0	0.0	1.1133855102896306E-4	5.566927551448153E-5
16	0.0	0.0	0.0	1.1133855102896306E-4	5.566927551448153E-5
17	0.0	0.0	0.0	1.1133855102896306E-4	5.566927551448153E-5
18	0.0	0.0	0.0	2.7834637757240765E-4	5.566927551448153E-5
19	0.0	0.0	0.0	3.3401565308688915E-4	5.566927551448153E-5
20	0.0	0.0	0.0	3.3401565308688915E-4	5.566927551448153E-5
21	0.0	0.0	0.0	3.896849286013707E-4	5.566927551448153E-5
22	0.0	0.0	0.0	4.453542041158522E-4	5.566927551448153E-5
23	0.0	0.0	0.0	5.010234796303338E-4	1.1133855102896306E-4
24	0.0	0.0	0.0	7.237005816882598E-4	1.1133855102896306E-4
25	0.0	0.0	0.0	8.350391327172229E-4	1.1133855102896306E-4
26	0.0	0.0	0.0	8.907084082317044E-4	1.1133855102896306E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	50	7.197592E-4	19.2002	12
TCTAGCG	440	0.0	18.544615	28
CTAGCGG	435	0.0	18.390997	29
TAGCGGC	460	0.0	17.391485	30
GTATCAA	2460	0.0	16.861198	1
CGAGCCG	480	0.0	16.333504	15
GTGTTAT	110	1.9997788E-7	16.014881	1
CGTTTAT	395	0.0	15.797633	31
CAAGACG	605	0.0	15.599125	4
AGCGGCG	525	0.0	15.54302	31
ATACCGT	515	0.0	15.5306835	6
TACCGTC	485	0.0	15.504022	7
CTTACAC	145	1.2259989E-9	15.444997	3
ATCGTTT	450	0.0	15.289048	29
ACACCGT	105	2.1913274E-6	15.23486	6
GTCTTAG	200	0.0	15.214138	1
CGCCGGT	575	0.0	15.024988	7
AAGACGG	630	0.0	14.980947	5
ACCGTCG	505	0.0	14.890001	8
GCGCAAG	645	0.0	14.649272	1
>>END_MODULE