FastQCFastQC Report
Thu 2 Feb 2017
SRR4063021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796323
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32490.18086947614655047No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26390.14691121808271676No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26060.14507413199073885No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23930.1332165763061543No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22450.12497752353001101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21990.12241673685634488No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21660.12057965076436698No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21560.12002295800922218No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21340.11879823394790358No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20970.11673847075386777No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20600.11467870755983195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20240.11267461364131062No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18940.10543760782442801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.017.718421
CTAGCGG5050.017.4252929
TCTAGCG5100.017.25445228
TATACCG652.0998644E-417.2322395
GTATTAT1404.0017767E-1117.1433641
TAGCGGC5500.016.58138730
CGAACGA2650.016.3014616
ACGAACG2800.015.99958215
CGATCGG1255.1435563E-815.35959915
GCATTCG7800.014.97396822
GATAACG2900.014.89657811
CTTACAC2150.014.8845753
CATCGTT4100.014.8288828
CGTTTAT3900.014.76884531
CCGATAA2950.014.6453179
CGGTCCA6600.014.5454810
GTCCTAT5750.014.4699931
AACGAAC3000.014.39962514
ATCGTTT3900.014.358629
TAAGAGG8600.014.3264044
Produced by FastQC (version 0.11.5)