##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1796323
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244294038432955	32.0	32.0	32.0	32.0	32.0
2	31.294989264180217	32.0	32.0	32.0	32.0	32.0
3	31.381978630791902	32.0	32.0	32.0	32.0	32.0
4	31.48168341662385	32.0	32.0	32.0	32.0	32.0
5	31.40316691374547	32.0	32.0	32.0	32.0	32.0
6	34.93631768896796	36.0	36.0	36.0	36.0	36.0
7	34.96017086014041	36.0	36.0	36.0	36.0	36.0
8	34.893103857157094	36.0	36.0	36.0	36.0	36.0
9	35.01026652779038	36.0	36.0	36.0	36.0	36.0
10	34.850471769275345	36.0	36.0	36.0	32.0	36.0
11	35.02700349547381	36.0	36.0	36.0	36.0	36.0
12	34.92200567492595	36.0	36.0	36.0	32.0	36.0
13	34.98230496408497	36.0	36.0	36.0	36.0	36.0
14	34.92334563438758	36.0	36.0	36.0	32.0	36.0
15	34.893539190891616	36.0	36.0	36.0	32.0	36.0
16	34.91042757900444	36.0	36.0	36.0	32.0	36.0
17	34.86999832435481	36.0	36.0	36.0	32.0	36.0
18	34.87198182064139	36.0	36.0	36.0	32.0	36.0
19	34.87083837372232	36.0	36.0	36.0	32.0	36.0
20	34.860122038185786	36.0	36.0	36.0	32.0	36.0
21	34.85598692440057	36.0	36.0	36.0	32.0	36.0
22	34.84182132055315	36.0	36.0	36.0	32.0	36.0
23	34.78565825856486	36.0	36.0	36.0	32.0	36.0
24	34.76003925797309	36.0	36.0	36.0	32.0	36.0
25	34.74390407515798	36.0	36.0	36.0	32.0	36.0
26	34.67621914321645	36.0	36.0	36.0	32.0	36.0
27	34.666789881329805	36.0	36.0	36.0	32.0	36.0
28	34.64310650144768	36.0	36.0	36.0	32.0	36.0
29	34.60973110069848	36.0	36.0	36.0	32.0	36.0
30	34.59091711234561	36.0	36.0	36.0	32.0	36.0
31	34.58553890363815	36.0	36.0	36.0	32.0	36.0
32	34.57326494177272	36.0	36.0	36.0	32.0	36.0
33	34.53588580672852	36.0	36.0	36.0	32.0	36.0
34	34.52510378144688	36.0	36.0	36.0	32.0	36.0
35	34.496597772226934	36.0	36.0	36.0	32.0	36.0
36	34.47237384367956	36.0	36.0	36.0	32.0	36.0
37	34.48178974494008	36.0	36.0	36.0	32.0	36.0
38	34.04546788077645	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	14.0
21	29.0
22	113.0
23	408.0
24	1124.0
25	2868.0
26	5866.0
27	11495.0
28	20200.0
29	31889.0
30	48535.0
31	70025.0
32	101145.0
33	163695.0
34	403576.0
35	935337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.994936971023705	18.236368960859792	12.052670199392258	26.716023868724243
2	16.09064739470574	20.075064451103728	36.354486359079075	27.47980179511146
3	18.08115801000154	23.707596016974676	28.502780401965573	29.708465571058213
4	12.345399291106657	15.68912237846131	35.503509673382425	36.4619686570496
5	14.56174641197602	36.50746552819287	33.060813673264775	15.869974386566335
6	35.22716708205271	35.37491350376686	15.963683550991165	13.434235863189262
7	30.87534104503826	30.09574590676367	20.48485874152502	18.544054306673043
8	28.395724276992297	32.91083076645581	18.915526022932937	19.77791893361896
9	27.338348978926046	13.959335191282262	18.235085840135483	40.467229989656204
10	15.904332398264659	26.38446854456069	31.06302221841193	26.648176838762726
11	38.34680395979633	20.872382190937362	21.575734834828484	19.205079014437825
12	24.80280485014616	23.559149081147705	28.051618951443423	23.58642711726271
13	29.428180629387096	18.94836050835069	25.13590292147442	26.4875559407878
14	23.65137004870505	19.437706915738428	24.496151304637305	32.41477173091922
15	25.392593648247004	27.000990356411403	21.73901909623158	25.86739689911002
16	26.30273063363326	25.688587186157502	23.04296053660728	24.965721643601956
17	24.629534888770007	25.81952132216756	24.48624217359573	25.064701615466706
18	25.6232314567035	24.51357578787334	25.458951424660263	24.404241330762897
19	25.90686641544978	24.780955318169394	24.93955708411015	24.37262118227067
20	26.030229529989874	24.015168764192186	24.42366990791745	25.530931797900493
21	27.257575074360403	24.02663446748601	24.096889249626596	24.618901208526996
22	26.19662499450266	23.94836563357481	24.677243457885915	25.17776591403662
23	24.52859178765476	23.77972744277189	25.380555802974968	26.31112496659838
24	25.111129791245784	24.70847392144954	24.76948744741341	25.410908839891267
25	25.164043620266085	24.264538465007092	24.85396541041384	25.71745250431298
26	25.0704355508447	25.1515456852693	25.11369057791945	24.664328185966554
27	25.901105648712008	24.285859821268023	24.38856974894799	25.424464781071983
28	24.90025457559693	24.343506151176598	25.409517108003403	25.346722165223067
29	24.91233480838357	24.644342916056853	25.246350461470456	25.196971814089114
30	24.83011128844868	24.7706008329237	25.467190477436407	24.93209740119121
31	25.50972180392947	24.573253251224862	24.311050963551654	25.60597398129401
32	25.33642334925289	24.38253031331225	24.234672717545784	26.046373619889074
33	24.72690045164483	24.201493829339153	24.943286925569623	26.128318793446393
34	25.6958798612499	24.220532721565107	25.05434713022101	25.029240286963983
35	26.195344601165825	24.08252858756471	25.06163980531341	24.660487005956057
36	24.842803883265983	24.915897642016496	24.64512228591406	25.59617618880346
37	26.05828684484917	24.55694215349912	24.531389956037973	24.853381045613734
38	24.995852636665365	24.262465192766108	25.08030297463372	25.661379195934803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	53.5
2	74.0
3	74.0
4	277.0
5	480.0
6	480.0
7	635.0
8	790.0
9	776.5
10	763.0
11	763.0
12	1064.5
13	1366.0
14	2039.5
15	2713.0
16	2713.0
17	4267.0
18	5821.0
19	5821.0
20	7302.5
21	8784.0
22	9910.0
23	11036.0
24	11036.0
25	13214.5
26	15393.0
27	15393.0
28	19571.5
29	23750.0
30	29306.5
31	34863.0
32	34863.0
33	45946.5
34	57030.0
35	57030.0
36	64556.0
37	72082.0
38	82737.0
39	93392.0
40	93392.0
41	101551.0
42	109710.0
43	125548.5
44	141387.0
45	141387.0
46	144551.5
47	147716.0
48	147716.0
49	158755.0
50	169794.0
51	173580.5
52	177367.0
53	177367.0
54	169135.5
55	160904.0
56	160904.0
57	155390.0
58	149876.0
59	134366.5
60	118857.0
61	118857.0
62	111997.5
63	105138.0
64	87693.5
65	70249.0
66	70249.0
67	59029.5
68	47810.0
69	47810.0
70	38225.5
71	28641.0
72	22277.5
73	15914.0
74	15914.0
75	12009.0
76	8104.0
77	8104.0
78	7689.0
79	7274.0
80	5596.5
81	3919.0
82	3919.0
83	3751.5
84	3584.0
85	3584.0
86	2230.0
87	876.0
88	737.0
89	598.0
90	598.0
91	367.5
92	137.0
93	92.0
94	47.0
95	47.0
96	39.5
97	32.0
98	32.0
99	25.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009185430459889453
2	0.0
3	0.0
4	1.1133855102896306E-4
5	0.0
6	1.1133855102896306E-4
7	3.3401565308688915E-4
8	0.002338109571608224
9	0.004286534214615078
10	0.0011133855102896306
11	0.003841180010499225
12	3.3401565308688915E-4
13	2.226771020579261E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.226771020579261E-4
22	0.0
23	1.6700782654344458E-4
24	0.0
25	1.1133855102896306E-4
26	0.0
27	1.1133855102896306E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.566927551448153E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1796323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.01475291464186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0105876995646	42.67737372232623
2	12.736941347016348	13.759654794947476
3	3.7654380722016	6.101676212560644
4	1.5147968244991352	3.272855047648197
5	0.8331395805161642	2.250091429249449
6	0.4915121496843208	1.5929344391845832
7	0.31833321142259124	1.2036282831661
8	0.22514982509373982	0.9729129736970552
9	0.16171533981310285	0.7861512710260886
>10	0.7965604070881526	7.553696945567602
>50	0.05870366543348414	2.2556875037252535
>100	0.0687865833196374	8.067458554114374
>500	0.012637886499217035	4.681123134477052
>1k	0.00569740784800768	4.824755688309812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3249	0.18086947614655047	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2639	0.14691121808271676	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2606	0.14507413199073885	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2393	0.1332165763061543	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2245	0.12497752353001101	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2199	0.12241673685634488	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2166	0.12057965076436698	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2156	0.12002295800922218	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2134	0.11879823394790358	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2097	0.11673847075386777	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2060	0.11467870755983195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2024	0.11267461364131062	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1894	0.10543760782442801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.566927551448153E-5
7	0.0	0.0	0.0	0.0	5.566927551448153E-5
8	0.0	0.0	0.0	0.0	5.566927551448153E-5
9	0.0	0.0	0.0	0.0	5.566927551448153E-5
10	0.0	0.0	0.0	0.0	5.566927551448153E-5
11	0.0	0.0	0.0	5.566927551448153E-5	5.566927551448153E-5
12	0.0	0.0	0.0	5.566927551448153E-5	5.566927551448153E-5
13	0.0	0.0	0.0	5.566927551448153E-5	1.1133855102896306E-4
14	0.0	0.0	0.0	5.566927551448153E-5	1.1133855102896306E-4
15	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
16	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
17	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
18	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
19	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
20	0.0	0.0	0.0	5.566927551448153E-5	1.6700782654344458E-4
21	0.0	0.0	0.0	1.1133855102896306E-4	1.6700782654344458E-4
22	0.0	0.0	0.0	1.6700782654344458E-4	1.6700782654344458E-4
23	0.0	0.0	0.0	2.226771020579261E-4	1.6700782654344458E-4
24	0.0	0.0	0.0	3.3401565308688915E-4	1.6700782654344458E-4
25	0.0	0.0	0.0	4.453542041158522E-4	1.6700782654344458E-4
26	0.0	0.0	0.0	5.010234796303338E-4	1.6700782654344458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2375	0.0	17.71842	1
CTAGCGG	505	0.0	17.42529	29
TCTAGCG	510	0.0	17.254452	28
TATACCG	65	2.0998644E-4	17.232239	5
GTATTAT	140	4.0017767E-11	17.143364	1
TAGCGGC	550	0.0	16.581387	30
CGAACGA	265	0.0	16.30146	16
ACGAACG	280	0.0	15.999582	15
CGATCGG	125	5.1435563E-8	15.359599	15
GCATTCG	780	0.0	14.973968	22
GATAACG	290	0.0	14.896578	11
CTTACAC	215	0.0	14.884575	3
CATCGTT	410	0.0	14.82888	28
CGTTTAT	390	0.0	14.768845	31
CCGATAA	295	0.0	14.645317	9
CGGTCCA	660	0.0	14.54548	10
GTCCTAT	575	0.0	14.469993	1
AACGAAC	300	0.0	14.399625	14
ATCGTTT	390	0.0	14.3586	29
TAAGAGG	860	0.0	14.326404	4
>>END_MODULE