FastQCFastQC Report
Thu 2 Feb 2017
SRR4063020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016135
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT64970.2154081299411333No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT52170.17296971123640023No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC50020.1658413830945896No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT41890.1388863562141615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38880.12890669681562664No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36520.12108211336694147No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA36450.12085002826464997No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35190.1166724964234028No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG34140.11319121988903016No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG34010.11276020469906023No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC33420.11080405883688892No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31940.10589711667415418No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG30530.10122225961371092No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC30440.10092386448219329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8650.019.42194628
CTAGCGG8700.019.31064429
TAGCGGC9450.017.60786430
GTATCAA47600.016.5868151
CAAGACG10650.016.5219694
TACCGTC6700.016.4762297
ATCGTTT5650.016.14184429
ACCGTCG6550.016.1207838
AGCGGCG10450.016.07600631
AAGACGG10950.016.0690465
CGGTCCA10900.016.0007810
CATCGTT5700.015.99998428
CGCCGGT10950.015.4872857
GCGCAAG11100.015.4377431
CGCTTCG6950.015.42393432
CGCAAGA11050.015.2086482
CGTCGTA7100.015.09932610
ATACCGT7350.015.0169076
TCTTGCG12100.014.8148152
GGCGTAA650.00417012114.7657876
Produced by FastQC (version 0.11.5)