##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063020_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3016135
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270950405071392	32.0	32.0	32.0	32.0	32.0
2	30.877102981133138	32.0	32.0	32.0	32.0	32.0
3	30.93083001921333	32.0	32.0	32.0	32.0	32.0
4	30.999640268091447	32.0	32.0	32.0	32.0	32.0
5	30.89135168021325	32.0	32.0	32.0	32.0	32.0
6	34.56186278134102	36.0	36.0	36.0	32.0	36.0
7	34.50270660961794	36.0	36.0	36.0	32.0	36.0
8	34.472850518958865	36.0	36.0	36.0	32.0	36.0
9	34.626318119049714	36.0	36.0	36.0	32.0	36.0
10	34.33289060337153	36.0	36.0	36.0	32.0	36.0
11	34.609655734905765	36.0	36.0	36.0	32.0	36.0
12	34.42299167643358	36.0	36.0	36.0	32.0	36.0
13	34.51610455102308	36.0	36.0	36.0	32.0	36.0
14	34.41044382960312	36.0	36.0	36.0	32.0	36.0
15	34.34962924404909	36.0	36.0	36.0	32.0	36.0
16	34.37005273305074	36.0	36.0	36.0	32.0	36.0
17	34.289716806442684	36.0	36.0	36.0	32.0	36.0
18	34.31593943905031	36.0	36.0	36.0	32.0	36.0
19	34.31140880630343	36.0	36.0	36.0	32.0	36.0
20	34.296022227121796	36.0	36.0	36.0	32.0	36.0
21	34.275621615080226	36.0	36.0	36.0	32.0	36.0
22	34.253129584716866	36.0	36.0	36.0	32.0	36.0
23	34.19588115253462	36.0	36.0	36.0	32.0	36.0
24	34.17964779427977	36.0	36.0	36.0	32.0	36.0
25	34.16138070742855	36.0	36.0	36.0	32.0	36.0
26	34.10684203459063	36.0	36.0	36.0	32.0	36.0
27	34.12009873563352	36.0	36.0	36.0	32.0	36.0
28	34.099148081899514	36.0	36.0	36.0	32.0	36.0
29	34.07051441662923	36.0	36.0	36.0	32.0	36.0
30	34.03849661901739	36.0	36.0	36.0	32.0	36.0
31	34.059232096706545	36.0	36.0	36.0	32.0	36.0
32	34.01894311759918	36.0	36.0	36.0	32.0	36.0
33	33.98204954353834	36.0	36.0	36.0	32.0	36.0
34	33.99328412023998	36.0	36.0	36.0	32.0	36.0
35	33.94807128991242	36.0	36.0	36.0	32.0	36.0
36	33.90787779724714	36.0	36.0	36.0	32.0	36.0
37	33.918064012386715	36.0	36.0	36.0	32.0	36.0
38	33.323687102865094	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	5.0
8	6.0
9	9.0
10	28.0
11	30.0
12	14.0
13	16.0
14	211.0
15	589.0
16	822.0
17	950.0
18	1342.0
19	1713.0
20	2319.0
21	3450.0
22	5379.0
23	8365.0
24	12553.0
25	19452.0
26	28202.0
27	40648.0
28	57016.0
29	77382.0
30	105083.0
31	142104.0
32	194580.0
33	294651.0
34	658359.0
35	1360854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06701178938772	16.915107445588397	11.205744152194805	25.81213661282907
2	17.121536065391588	18.822407367169188	37.4293860747631	26.626670492676123
3	19.729550672525594	22.52844688433833	27.655086749899528	30.086915693236545
4	12.533093726739066	14.657060298966694	35.66675674017881	37.14308923411542
5	14.981880754476764	36.04891035863839	33.358409617622584	15.610799269262266
6	35.467801454181355	34.92717488967651	16.254272859591595	13.350750796550548
7	31.566568328860722	29.83905887470517	19.880582228773655	18.713790567660453
8	28.739586793902898	32.765816502117616	18.701697079892657	19.792899624086825
9	27.20755644072253	14.457471812379378	18.285358484335383	40.04961326256271
10	15.90063256787945	26.166109493690403	31.044431602333994	26.888826336096155
11	38.348735244072095	21.17038827934044	21.50144757625471	18.97942890033275
12	25.21827382892398	23.418602669517195	28.04776842301407	23.31535507854476
13	29.25755866025713	19.00197399420966	25.28460102138367	26.455866324149547
14	23.48675482525176	19.737633100817582	24.64231512610197	32.13329694782869
15	25.369641340534976	27.127305190681373	21.918516904593485	25.584536564190163
16	26.227922007728665	25.84056030500838	23.252876696158147	24.678640991104807
17	24.265954084761034	25.941987316917555	24.69536692430111	25.096691674020306
18	25.333368258653383	24.74973963505472	25.827406804933357	24.08948530135854
19	25.54901649362229	24.94000947646352	25.311275963702958	24.199698066211234
20	25.774637006247758	24.300778425185207	24.84683986298231	25.07774470558472
21	27.03589520502025	24.10374721563317	24.44112063014391	24.41923694920267
22	25.813129100958214	24.126474554454692	25.169244052626222	24.891152291960868
23	24.333476908732134	24.061181025367087	25.71786870444258	25.887473361458195
24	24.656838346696148	25.029586760252236	25.228412441924895	25.08516245112672
25	24.917761739843243	24.59275480928019	25.218030871181725	25.27145257969484
26	24.620437355914756	25.59610502272203	25.664516497014166	24.118941124349046
27	25.409590643119728	24.662643735139067	24.968624383554104	24.9591412381871
28	24.497588491071152	24.7037255873266	25.80755542027498	24.991130501327273
29	24.266708313728376	25.058433546575575	25.80410163872567	24.870756500970383
30	24.43061834494626	25.196723512823954	25.88584018421537	24.486817958014413
31	25.07893014425267	25.17445534718985	24.647558089336787	25.099056419220695
32	24.822857059490186	24.82895800493779	24.679252740286067	25.668932195285958
33	24.34362223835908	24.614754614754613	25.348539559065873	25.693083587820432
34	25.279515874989638	24.749896377352236	25.380751056951006	24.58983669070712
35	25.764796703052557	24.559811224026227	25.51294286648464	24.16244920643658
36	24.306447644432183	25.33748316314654	25.097406710070548	25.258662482350736
37	25.72991272746565	24.917104355668734	24.835899782481828	24.51708313438379
38	24.447984148257294	24.687643611656178	25.556160488284608	25.308211751801924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	157.0
2	227.0
3	227.0
4	584.5
5	942.0
6	942.0
7	1213.5
8	1485.0
9	1549.0
10	1613.0
11	1613.0
12	2192.5
13	2772.0
14	4117.0
15	5462.0
16	5462.0
17	8037.0
18	10612.0
19	10612.0
20	13438.0
21	16264.0
22	18356.0
23	20448.0
24	20448.0
25	25170.0
26	29892.0
27	29892.0
28	38255.0
29	46618.0
30	56624.0
31	66630.0
32	66630.0
33	83935.0
34	101240.0
35	101240.0
36	114613.5
37	127987.0
38	146569.0
39	165151.0
40	165151.0
41	175766.5
42	186382.0
43	210295.5
44	234209.0
45	234209.0
46	239998.0
47	245787.0
48	245787.0
49	261370.0
50	276953.0
51	282315.5
52	287678.0
53	287678.0
54	275619.5
55	263561.0
56	263561.0
57	253300.5
58	243040.0
59	218279.5
60	193519.0
61	193519.0
62	182627.0
63	171735.0
64	143757.0
65	115779.0
66	115779.0
67	97660.5
68	79542.0
69	79542.0
70	63603.0
71	47664.0
72	37028.0
73	26392.0
74	26392.0
75	19877.0
76	13362.0
77	13362.0
78	12863.0
79	12364.0
80	9524.0
81	6684.0
82	6684.0
83	6702.5
84	6721.0
85	6721.0
86	4518.5
87	2316.0
88	1964.0
89	1612.0
90	1612.0
91	1189.5
92	767.0
93	703.5
94	640.0
95	640.0
96	569.5
97	499.0
98	499.0
99	999.0
100	1499.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12201045377610752
2	0.05570042454996212
3	0.011239549953831642
4	0.0026524011690458155
5	1.6577507306536347E-4
6	1.6577507306536347E-4
7	3.315501461307269E-5
8	4.31015189969945E-4
9	1.9893008767843615E-4
10	5.304802338091631E-4
11	0.0019561458621712886
12	0.002785021227498106
13	0.013991416166716676
14	0.009349714120886499
15	0.023606370404507755
16	0.011571100099962369
17	0.020622419089331216
18	0.005835282571900794
19	0.008023513536363592
20	0.005768972542674648
21	0.006266297761870739
22	0.007426723273328282
23	0.009880194354695662
24	0.01419034625439511
25	0.016809592408827855
26	0.018533653168707635
27	0.008553993770172755
28	0.006001057644966157
29	0.01047698461773097
30	0.003315501461307269
31	0.005868437586513867
32	0.0069293980541321925
33	0.008786078872464263
34	0.012764680626032985
35	0.016478042262697126
36	0.014223501269008183
37	0.009780729310856443
38	0.006266297761870739
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3016135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.34351491957838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32808570380695	32.342661182955354
2	15.227380242459516	13.809258864201121
3	5.800773309882199	7.890823533652067
4	2.7515305052326537	4.990562580627675
5	1.4696072218017742	3.331857849384444
6	0.8710898490502584	2.369896534002221
7	0.5670003267180899	1.7996851441763269
8	0.3701380788832668	1.3426689201717987
9	0.2675683116804582	1.0919238959420328
>10	1.1696928477990327	9.11531999237746
>50	0.07783001772872063	2.4752544937093583
>100	0.07633096258484226	7.464065134779204
>500	0.013578067536352266	4.238256027957002
>1k	0.009174369956893304	7.181459798552065
>5k	2.201848789654393E-4	0.5563060475119157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6497	0.2154081299411333	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5217	0.17296971123640023	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5002	0.1658413830945896	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4189	0.1388863562141615	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3888	0.12890669681562664	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3652	0.12108211336694147	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3645	0.12085002826464997	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3519	0.1166724964234028	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3414	0.11319121988903016	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3401	0.11276020469906023	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3342	0.11080405883688892	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3194	0.10589711667415418	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3053	0.10122225961371092	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3044	0.10092386448219329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3262005845229077E-4
2	0.0	0.0	0.0	0.0	1.3262005845229077E-4
3	0.0	0.0	0.0	0.0	1.3262005845229077E-4
4	0.0	0.0	0.0	3.315501461307269E-5	1.3262005845229077E-4
5	3.315501461307269E-5	0.0	0.0	3.315501461307269E-5	1.3262005845229077E-4
6	3.315501461307269E-5	0.0	0.0	3.315501461307269E-5	2.3208510229150883E-4
7	3.315501461307269E-5	0.0	0.0	3.315501461307269E-5	2.3208510229150883E-4
8	3.315501461307269E-5	0.0	0.0	3.315501461307269E-5	2.6524011690458153E-4
9	3.315501461307269E-5	0.0	0.0	3.315501461307269E-5	2.6524011690458153E-4
10	6.631002922614538E-5	0.0	0.0	1.3262005845229077E-4	2.983951315176542E-4
11	6.631002922614538E-5	0.0	0.0	1.3262005845229077E-4	2.983951315176542E-4
12	6.631002922614538E-5	0.0	3.315501461307269E-5	1.3262005845229077E-4	5.636352484222357E-4
13	6.631002922614538E-5	0.0	3.315501461307269E-5	1.3262005845229077E-4	6.299452776483811E-4
14	6.631002922614538E-5	0.0	3.315501461307269E-5	1.3262005845229077E-4	6.962553068745266E-4
15	6.631002922614538E-5	0.0	3.315501461307269E-5	1.3262005845229077E-4	7.957203507137446E-4
16	6.631002922614538E-5	0.0	3.315501461307269E-5	1.3262005845229077E-4	8.288753653268173E-4
17	6.631002922614538E-5	0.0	3.315501461307269E-5	1.6577507306536344E-4	8.288753653268173E-4
18	6.631002922614538E-5	0.0	3.315501461307269E-5	1.9893008767843615E-4	8.620303799398899E-4
19	6.631002922614538E-5	0.0	3.315501461307269E-5	2.6524011690458153E-4	8.620303799398899E-4
20	6.631002922614538E-5	0.0	3.315501461307269E-5	4.973252191960904E-4	9.946504383921808E-4
21	6.631002922614538E-5	0.0	3.315501461307269E-5	5.636352484222357E-4	0.0010941154822313988
22	6.631002922614538E-5	0.0	3.315501461307269E-5	7.294103214875992E-4	0.0012267355406836895
23	6.631002922614538E-5	0.0	3.315501461307269E-5	0.0010941154822313988	0.0012930455699098349
24	6.631002922614538E-5	0.0	3.315501461307269E-5	0.0013925106137490531	0.0013925106137490531
25	6.631002922614538E-5	0.0	3.315501461307269E-5	0.0017903707891059252	0.0013925106137490531
26	6.631002922614538E-5	0.0	3.315501461307269E-5	0.0021219209352366522	0.0015251306722013438
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	865	0.0	19.421946	28
CTAGCGG	870	0.0	19.310644	29
TAGCGGC	945	0.0	17.607864	30
GTATCAA	4760	0.0	16.586815	1
CAAGACG	1065	0.0	16.521969	4
TACCGTC	670	0.0	16.476229	7
ATCGTTT	565	0.0	16.141844	29
ACCGTCG	655	0.0	16.120783	8
AGCGGCG	1045	0.0	16.076006	31
AAGACGG	1095	0.0	16.069046	5
CGGTCCA	1090	0.0	16.00078	10
CATCGTT	570	0.0	15.999984	28
CGCCGGT	1095	0.0	15.487285	7
GCGCAAG	1110	0.0	15.437743	1
CGCTTCG	695	0.0	15.423934	32
CGCAAGA	1105	0.0	15.208648	2
CGTCGTA	710	0.0	15.099326	10
ATACCGT	735	0.0	15.016907	6
TCTTGCG	1210	0.0	14.814815	2
GGCGTAA	65	0.004170121	14.765787	6
>>END_MODULE