FastQCFastQC Report
Thu 2 Feb 2017
SRR4063020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016135
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT65510.21719850073023922No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC52830.175157942200863No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT52620.1744616868939885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43200.14322966312847402No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT39950.1324542833792254No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC39270.13019974238553644No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC38640.12811097646491287No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA37870.12555804033970627No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC35940.11915912251938324No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG34080.11299228980135173No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33780.11199763936295955No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33180.11000833848617518No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC31890.10573134160108881No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG31080.10304578541742992No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG30730.10188535990597239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8750.019.93109928
CTAGCGG8850.019.525129
ACCGTCG6400.019.2509578
TAGCGGC9250.018.8537430
ATACCGT7000.018.058046
TACCGTC6650.018.0460117
CGTCGTA7050.017.02184110
CAAGACG9850.016.7309654
AAGACGG9950.016.724455
AGCGGCG10550.016.68218831
CCGTCGT7300.016.6583639
CGCAAGA9550.016.0835022
CGCCGGT11250.015.7874527
GCGGCGC11600.015.72387732
GTATCAA52550.015.7115331
GTCGTAG7700.015.58493311
GTTTTCG8550.015.34477428
GCGCAAG10350.015.3051081
CGGTCCA11850.015.2578910
CGAAAAA7500.015.14641624
Produced by FastQC (version 0.11.5)