##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3016135
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197726229097835	32.0	32.0	32.0	32.0	32.0
2	31.25042645637546	32.0	32.0	32.0	32.0	32.0
3	31.36020138355876	32.0	32.0	32.0	32.0	32.0
4	31.464092290298677	32.0	32.0	32.0	32.0	32.0
5	31.371436291810546	32.0	32.0	32.0	32.0	32.0
6	34.90857769960562	36.0	36.0	36.0	36.0	36.0
7	34.94203077780006	36.0	36.0	36.0	36.0	36.0
8	34.86761501060131	36.0	36.0	36.0	32.0	36.0
9	34.99654557902746	36.0	36.0	36.0	36.0	36.0
10	34.82444519227422	36.0	36.0	36.0	32.0	36.0
11	35.01348878614518	36.0	36.0	36.0	36.0	36.0
12	34.90897688598156	36.0	36.0	36.0	32.0	36.0
13	34.96904283130563	36.0	36.0	36.0	36.0	36.0
14	34.90778861025783	36.0	36.0	36.0	32.0	36.0
15	34.87676811548555	36.0	36.0	36.0	32.0	36.0
16	34.89021512631232	36.0	36.0	36.0	32.0	36.0
17	34.85344455735569	36.0	36.0	36.0	32.0	36.0
18	34.85515834006104	36.0	36.0	36.0	32.0	36.0
19	34.852933307030355	36.0	36.0	36.0	32.0	36.0
20	34.83901648964652	36.0	36.0	36.0	32.0	36.0
21	34.83311025534334	36.0	36.0	36.0	32.0	36.0
22	34.816488651867374	36.0	36.0	36.0	32.0	36.0
23	34.76190422510929	36.0	36.0	36.0	32.0	36.0
24	34.734366996172255	36.0	36.0	36.0	32.0	36.0
25	34.71319121988903	36.0	36.0	36.0	32.0	36.0
26	34.65066019922848	36.0	36.0	36.0	32.0	36.0
27	34.638117657200354	36.0	36.0	36.0	32.0	36.0
28	34.60979498596714	36.0	36.0	36.0	32.0	36.0
29	34.577605445379604	36.0	36.0	36.0	32.0	36.0
30	34.54972804599264	36.0	36.0	36.0	32.0	36.0
31	34.54817937526006	36.0	36.0	36.0	32.0	36.0
32	34.5329923892664	36.0	36.0	36.0	32.0	36.0
33	34.49397490496944	36.0	36.0	36.0	32.0	36.0
34	34.493898316885684	36.0	36.0	36.0	32.0	36.0
35	34.464088974797214	36.0	36.0	36.0	32.0	36.0
36	34.4358050949311	36.0	36.0	36.0	32.0	36.0
37	34.43482072254724	36.0	36.0	36.0	32.0	36.0
38	33.997787234324726	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	11.0
21	64.0
22	226.0
23	689.0
24	1958.0
25	4864.0
26	10143.0
27	19696.0
28	34133.0
29	54227.0
30	82272.0
31	120092.0
32	175180.0
33	284920.0
34	701006.0
35	1526649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29216109331081	18.138432497189033	11.925293787822872	26.64411262167728
2	16.100870816458812	19.796229280188054	36.64242482514874	27.46047507820439
3	18.598073362100834	23.279727200539764	28.027226898000258	30.094972539359148
4	12.073804437669027	15.591255425407303	35.583841959223946	36.751098177699724
5	14.468417361954952	36.64242482514874	33.019742153451354	15.869415659444952
6	35.515909460136	35.33902716394851	15.921959597232217	13.22310377868328
7	31.357432893243555	30.009246958102224	19.804769494122763	18.828550654531455
8	28.632866157902885	32.99977553563927	18.453755534107476	19.913602772350366
9	26.991932403541625	14.301615110795169	18.083790006419065	40.62266247924414
10	15.627232799301618	26.490055246603823	31.000993663035835	26.881718291058725
11	38.51318149716344	21.052563520194166	21.380351926577518	19.05390305606488
12	25.010046006066062	23.536068145739367	27.995301254192466	23.4585845940021
13	29.269845311604815	19.03543218324952	25.211827879983872	26.482894625161794
14	23.380087429773535	19.70054390801473	24.663915905620936	32.2554527565908
15	25.349329522716985	27.27603373191187	21.77240077118564	25.602235974185504
16	26.247830418731255	25.93551018107611	23.118295434388713	24.698363965803917
17	24.24294668507875	26.120879867777802	24.55108939089265	25.0850840562508
18	25.288854776062742	24.81036160516688	25.733297746950985	24.167485871819398
19	25.48539770268904	25.04344135789678	25.23819391373397	24.232967025680217
20	25.7866110104488	24.21778202898743	24.760529618203428	25.23507734236034
21	27.02375094989211	24.188132349645176	24.31246377811051	24.475652922352204
22	25.681825038882565	24.27058090608073	25.11709530050565	24.93049875453105
23	24.232328028083653	24.064895037750336	25.712170422249425	25.990606511916585
24	24.651084914965676	25.081304384584907	25.129909636007675	25.137701064441742
25	24.921497214149895	24.546414533832206	25.266110436038176	25.265977815979724
26	24.57733130468716	25.589024091444244	25.583652975515335	24.24999162835326
27	25.40460735497826	24.65580110167519	24.89733546321218	25.042256080134372
28	24.461537696422738	24.704895503682696	25.81107941123325	25.022487388661318
29	24.381402026102943	24.98399441669554	25.67676844703569	24.957835110165824
30	24.38136887108833	25.17122078421556	25.84075314931195	24.60665719538416
31	25.099208092475966	25.05196219665234	24.696639905044037	25.152189805827657
32	24.870007476455797	24.804360547521913	24.689710506989908	25.635921469032386
33	24.323214975457	24.58099521407364	25.370913437230097	25.724876373239265
34	25.205801464456997	24.700253801636862	25.458840535983967	24.635104197922175
35	25.764065600511916	24.484480966534985	25.471041581361575	24.280411851591523
36	24.382098281409817	25.29840342027131	25.07536963696917	25.244128661349706
37	25.66983904898156	24.9468608003289	24.79743114946778	24.585869001221763
38	24.503818464299	24.53634354441812	25.55045631261741	25.409381678665472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	76.0
2	88.0
3	88.0
4	349.0
5	610.0
6	610.0
7	958.0
8	1306.0
9	1398.0
10	1490.0
11	1490.0
12	2045.0
13	2600.0
14	4023.0
15	5446.0
16	5446.0
17	8128.0
18	10810.0
19	10810.0
20	13311.0
21	15812.0
22	18227.5
23	20643.0
24	20643.0
25	25236.0
26	29829.0
27	29829.0
28	38329.0
29	46829.0
30	56518.5
31	66208.0
32	66208.0
33	84487.5
34	102767.0
35	102767.0
36	115549.0
37	128331.0
38	146602.5
39	164874.0
40	164874.0
41	175629.5
42	186385.0
43	211189.5
44	235994.0
45	235994.0
46	241013.0
47	246032.0
48	246032.0
49	262297.0
50	278562.0
51	282339.5
52	286117.0
53	286117.0
54	275691.5
55	265266.0
56	265266.0
57	254730.5
58	244195.0
59	219451.5
60	194708.0
61	194708.0
62	183906.5
63	173105.0
64	144424.5
65	115744.0
66	115744.0
67	97683.0
68	79622.0
69	79622.0
70	63397.0
71	47172.0
72	36455.0
73	25738.0
74	25738.0
75	19224.0
76	12710.0
77	12710.0
78	12333.5
79	11957.0
80	9262.5
81	6568.0
82	6568.0
83	6107.0
84	5646.0
85	5646.0
86	3596.0
87	1546.0
88	1283.5
89	1021.0
90	1021.0
91	612.5
92	204.0
93	138.5
94	73.0
95	73.0
96	48.0
97	23.0
98	23.0
99	31.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00888554391630348
2	0.0
3	0.0
4	6.631002922614538E-5
5	0.0
6	1.6577507306536347E-4
7	2.6524011690458153E-4
8	0.0021882309644627976
9	0.00394544673895565
10	7.294103214875992E-4
11	0.005470577411156995
12	3.647051607437996E-4
13	2.3208510229150883E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.946504383921807E-5
22	6.631002922614538E-5
23	9.946504383921807E-5
24	0.0
25	0.0
26	6.631002922614538E-5
27	3.315501461307269E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.315501461307269E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3016135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.19642381436879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00154574119671	29.806200136832867
2	16.328424460053707	14.106590863947716
3	6.326128335914982	8.197983621068133
4	3.029819619953013	5.235094895383206
5	1.599998820619396	3.4557113578982825
6	0.9560594979428017	2.477901075893394
7	0.6043936045245408	1.8275349604215247
8	0.41964554285862443	1.4501749376905602
9	0.2921698378090292	1.1358622925796793
>10	1.2494685275927089	9.387155434515208
>50	0.08577325645693354	2.5743526371386003
>100	0.0810239959386465	7.534534509440786
>500	0.015360130116781058	4.56349815228839
>1k	0.009957069271604767	7.6773929643765575
>5k	2.3155975050243645E-4	0.5700121605250589
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6551	0.21719850073023922	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5283	0.175157942200863	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5262	0.1744616868939885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4320	0.14322966312847402	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3995	0.1324542833792254	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3927	0.13019974238553644	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3864	0.12811097646491287	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3787	0.12555804033970627	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3594	0.11915912251938324	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3408	0.11299228980135173	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3378	0.11199763936295955	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3318	0.11000833848617518	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3189	0.10573134160108881	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	3108	0.10304578541742992	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3073	0.10188535990597239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6577507306536344E-4
2	0.0	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4
3	0.0	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4
4	0.0	0.0	0.0	6.631002922614538E-5	1.6577507306536344E-4
5	0.0	0.0	0.0	6.631002922614538E-5	1.6577507306536344E-4
6	0.0	0.0	0.0	6.631002922614538E-5	2.6524011690458153E-4
7	0.0	0.0	0.0	6.631002922614538E-5	2.6524011690458153E-4
8	0.0	0.0	0.0	6.631002922614538E-5	2.983951315176542E-4
9	0.0	0.0	0.0	6.631002922614538E-5	3.978601753568723E-4
10	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	4.3101518996994497E-4
11	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	4.3101518996994497E-4
12	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	6.299452776483811E-4
13	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	8.288753653268173E-4
14	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	8.951853945529626E-4
15	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	9.283404091660353E-4
16	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	9.283404091660353E-4
17	0.0	0.0	3.315501461307269E-5	1.6577507306536344E-4	9.283404091660353E-4
18	0.0	0.0	3.315501461307269E-5	1.9893008767843615E-4	9.61495423779108E-4
19	0.0	0.0	3.315501461307269E-5	2.6524011690458153E-4	9.946504383921808E-4
20	0.0	0.0	3.315501461307269E-5	5.636352484222357E-4	0.0011272704968444715
21	0.0	0.0	3.315501461307269E-5	5.967902630353084E-4	0.0011935805260706168
22	0.0	0.0	3.315501461307269E-5	7.294103214875992E-4	0.0013262005845229075
23	3.315501461307269E-5	0.0	3.315501461307269E-5	0.0010609604676183261	0.0015914407014274892
24	3.315501461307269E-5	0.0	3.315501461307269E-5	0.0013593555991359802	0.0016245957160405619
25	3.315501461307269E-5	0.0	3.315501461307269E-5	0.0017903707891059252	0.0016245957160405619
26	3.315501461307269E-5	0.0	3.315501461307269E-5	0.002055610906010507	0.0016577507306536345
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	875	0.0	19.931099	28
CTAGCGG	885	0.0	19.5251	29
ACCGTCG	640	0.0	19.250957	8
TAGCGGC	925	0.0	18.85374	30
ATACCGT	700	0.0	18.05804	6
TACCGTC	665	0.0	18.046011	7
CGTCGTA	705	0.0	17.021841	10
CAAGACG	985	0.0	16.730965	4
AAGACGG	995	0.0	16.72445	5
AGCGGCG	1055	0.0	16.682188	31
CCGTCGT	730	0.0	16.658363	9
CGCAAGA	955	0.0	16.083502	2
CGCCGGT	1125	0.0	15.787452	7
GCGGCGC	1160	0.0	15.723877	32
GTATCAA	5255	0.0	15.711533	1
GTCGTAG	770	0.0	15.584933	11
GTTTTCG	855	0.0	15.344774	28
GCGCAAG	1035	0.0	15.305108	1
CGGTCCA	1185	0.0	15.25789	10
CGAAAAA	750	0.0	15.146416	24
>>END_MODULE