FastQCFastQC Report
Thu 2 Feb 2017
SRR4063019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091335
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62870.5760834207644765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40930.37504524275314177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22260.2039703665693852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.16896736565765783No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18050.16539376085253382No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17540.1607205853381409No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15760.1444102864839852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13630.12489290639446182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC401.5954155E-423.9957033
TACGGGG450.00885211117.77703921
CCGGACT450.00886380217.7729684
TAGCGTA1101.0635631E-817.4538237
CGTGCGC957.69116E-716.84217810
ATAGCGT1151.8444553E-816.6926656
TTAGGAC4500.016.3526293
ACGTTTT2850.016.28002529
AGCGTAT1304.802132E-915.9993358
AATAGCG1203.1161107E-815.9971375
GTCCTAA5350.015.867261
GACGTGA3550.015.7739927
CGGTAGG756.1940274E-414.9491561
TTAATAC1401.2809323E-814.8544853
TCCAATA1851.0913936E-1114.7088332
GTGCGCC1002.3253846E-514.40006211
ACGAACG1002.3253846E-514.40006215
AACCGCG1002.3263903E-514.3994027
TAGGACC5000.014.3961034
TATGGCG7200.014.22228416
Produced by FastQC (version 0.11.5)