##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063019_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091335
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137853179821043	32.0	32.0	32.0	32.0	32.0
2	30.789410217760818	32.0	32.0	32.0	32.0	32.0
3	30.860393921206594	32.0	32.0	32.0	32.0	32.0
4	30.905733803094375	32.0	32.0	32.0	32.0	32.0
5	30.860352687305	32.0	32.0	32.0	32.0	32.0
6	34.482164504941196	36.0	36.0	36.0	32.0	36.0
7	34.377270957130484	36.0	36.0	36.0	32.0	36.0
8	34.35958252965405	36.0	36.0	36.0	32.0	36.0
9	34.46129648549712	36.0	36.0	36.0	32.0	36.0
10	34.23491595156391	36.0	36.0	36.0	32.0	36.0
11	34.51177777675966	36.0	36.0	36.0	32.0	36.0
12	34.34581132282938	36.0	36.0	36.0	32.0	36.0
13	34.416091301021225	36.0	36.0	36.0	32.0	36.0
14	34.329208721428344	36.0	36.0	36.0	32.0	36.0
15	34.30120632069896	36.0	36.0	36.0	32.0	36.0
16	34.304373084341655	36.0	36.0	36.0	32.0	36.0
17	34.24875771417576	36.0	36.0	36.0	32.0	36.0
18	34.26012910792745	36.0	36.0	36.0	32.0	36.0
19	34.24450970600228	36.0	36.0	36.0	32.0	36.0
20	34.20183261784878	36.0	36.0	36.0	32.0	36.0
21	34.18186441376846	36.0	36.0	36.0	32.0	36.0
22	34.16244874396954	36.0	36.0	36.0	32.0	36.0
23	34.12997109045344	36.0	36.0	36.0	32.0	36.0
24	34.126348004966395	36.0	36.0	36.0	32.0	36.0
25	34.0982548896535	36.0	36.0	36.0	32.0	36.0
26	34.07334961308856	36.0	36.0	36.0	32.0	36.0
27	34.07624514928963	36.0	36.0	36.0	32.0	36.0
28	34.041889062478525	36.0	36.0	36.0	32.0	36.0
29	34.00973303339488	36.0	36.0	36.0	32.0	36.0
30	33.97710510521517	36.0	36.0	36.0	32.0	36.0
31	33.97919887110741	36.0	36.0	36.0	32.0	36.0
32	33.9370550747479	36.0	36.0	36.0	32.0	36.0
33	33.90236911672401	36.0	36.0	36.0	32.0	36.0
34	33.874131224601065	36.0	36.0	36.0	32.0	36.0
35	33.8173677193529	36.0	36.0	36.0	32.0	36.0
36	33.78834088524605	36.0	36.0	36.0	32.0	36.0
37	33.760070922310746	36.0	36.0	36.0	27.0	36.0
38	33.17048110800075	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	5.0
10	9.0
11	7.0
12	6.0
13	7.0
14	280.0
15	535.0
16	569.0
17	730.0
18	842.0
19	1071.0
20	1306.0
21	1751.0
22	2558.0
23	3691.0
24	5246.0
25	7376.0
26	10630.0
27	15385.0
28	21118.0
29	28612.0
30	39093.0
31	52300.0
32	70593.0
33	105535.0
34	233011.0
35	489067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.452235166323234	17.79389827025301	11.735747064641197	25.018119498782553
2	15.274438937267695	20.812727262725335	39.905730092106154	24.00710370790081
3	18.547324356175782	26.30941318568127	28.86931435996972	26.27394809817323
4	11.918288729283995	16.62475969159887	37.43468834589015	34.02226323322698
5	13.145838029420872	38.11005613313615	34.23562355795751	14.508482279485474
6	31.314485417819693	37.101450337752404	18.328977799606353	13.255086444821556
7	28.1638801686743	31.894543741879204	22.112662118104996	17.828913971341496
8	26.877589150149404	34.070535757646844	20.27749550320571	18.77437958899804
9	27.797173001116064	14.587559812119846	19.265046264479984	38.35022092228411
10	15.142688017945044	27.794841533099003	33.20309493353077	23.85937551542519
11	35.344299909467104	22.2192288942891	23.75572244885991	18.680748747383895
12	24.677657157884994	24.772405931603114	30.254364720468207	20.295572190043682
13	29.66245530043788	21.201659863596454	25.552753273080185	23.58313156288548
14	23.005796238172696	20.82540264381773	27.168274187266604	29.00052693074297
15	23.817523754008647	28.73339144094974	24.35598155587267	23.09310324916894
16	23.387970329966404	27.057226801270524	26.31300162573016	23.24180124303291
17	22.370399969938788	26.786373838679435	27.127220336777235	23.716005854604543
18	22.798386113908542	25.932873261558225	29.118951248947333	22.149789375585897
19	24.220742387490457	25.868007987075526	27.597013735460656	22.31423588997336
20	24.866165110375984	25.138233434438774	27.628726163094374	22.366875292090864
21	25.370346952277796	25.245813608681228	26.227418595146588	23.156420843894384
22	24.463073318200937	26.12757581394667	26.680974325937886	22.728376541914507
23	22.787734496065422	26.13196386073241	27.410346006270032	23.669955636932137
24	23.315578788389775	26.34561244015631	27.641370411372684	22.697438360081236
25	23.45017454238186	25.917871141990155	27.461670786215137	23.170283529412846
26	22.55818535914767	26.79124756558598	27.95005155229694	22.70051552296941
27	23.405822933056704	26.645082598922144	27.065339610002226	22.883754858018925
28	22.479310973637357	26.570290457344086	27.458605911045662	23.491792657972894
29	22.84239397885488	26.696150458981272	27.399674309786924	23.061781252376925
30	22.805040194922235	26.51820248564336	27.734450525837367	22.942306793597037
31	23.32032109082912	26.33760354812697	26.94688805806026	23.395187302983654
32	22.711961013945324	26.960986454215398	26.88465281481441	23.442399717024877
33	22.34795063601899	26.706749471473305	27.477614935655826	23.46768495685188
34	22.844778999980754	26.86466904753395	27.69357376610499	22.596978186380305
35	23.394055257347986	26.810362287826866	27.338429490381237	22.457152964443903
36	22.612324797510187	27.2536900893173	27.327556027222254	22.80642908595026
37	23.15779827443218	27.314648082218774	26.48943628091108	23.038117362437973
38	22.60458561161495	27.778862128338844	26.862143000486583	22.75440925955962
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1599.0
1	1102.5
2	606.0
3	606.0
4	1259.5
5	1913.0
6	1913.0
7	2238.5
8	2564.0
9	2375.0
10	2186.0
11	2186.0
12	2938.0
13	3690.0
14	4399.5
15	5109.0
16	5109.0
17	7324.0
18	9539.0
19	9539.0
20	11289.0
21	13039.0
22	13204.0
23	13369.0
24	13369.0
25	15501.5
26	17634.0
27	17634.0
28	22207.0
29	26780.0
30	33212.5
31	39645.0
32	39645.0
33	46104.0
34	52563.0
35	52563.0
36	56854.5
37	61146.0
38	66578.0
39	72010.0
40	72010.0
41	74543.0
42	77076.0
43	82367.0
44	87658.0
45	87658.0
46	92865.0
47	98072.0
48	98072.0
49	97610.0
50	97148.0
51	93195.0
52	89242.0
53	89242.0
54	83563.0
55	77884.0
56	77884.0
57	73930.5
58	69977.0
59	62067.0
60	54157.0
61	54157.0
62	49295.5
63	44434.0
64	36237.5
65	28041.0
66	28041.0
67	22956.5
68	17872.0
69	17872.0
70	14296.5
71	10721.0
72	8379.5
73	6038.0
74	6038.0
75	4515.0
76	2992.0
77	2992.0
78	2699.0
79	2406.0
80	1866.5
81	1327.0
82	1327.0
83	1319.5
84	1312.0
85	1312.0
86	862.0
87	412.0
88	362.0
89	312.0
90	312.0
91	235.5
92	159.0
93	143.5
94	128.0
95	128.0
96	117.0
97	106.0
98	106.0
99	287.5
100	469.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12361007390031475
2	0.05836887848369199
3	0.011087337985128306
4	0.0026572958807332303
5	9.163089243907692E-5
6	2.7489267731723073E-4
7	9.163089243907692E-5
8	1.8326178487815384E-4
9	9.163089243907692E-5
10	2.7489267731723073E-4
11	0.001740986956342461
12	0.0025656649882941536
13	0.014752573682691382
14	0.01007939816829846
15	0.02345750846440369
16	0.012186908694397229
17	0.020891843476109537
18	0.005131329976588307
19	0.0065974242556135375
20	0.005956008008539999
21	0.005589484438783692
22	0.007147209610247999
23	0.010262659953176614
24	0.01456931189781323
25	0.017959654918059075
26	0.01924248741220615
27	0.008979827459029538
28	0.005131329976588307
29	0.010445921738054769
30	0.0025656649882941536
31	0.00503969908414923
32	0.006689055148052614
33	0.008613303889273229
34	0.01246180137171446
35	0.017043345993668305
36	0.015485620822204
37	0.00916308924390769
38	0.004764806406831999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1091335.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.182885232019345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72040523829715	46.774382286424725
2	13.78982721252921	16.598231770020455
3	4.296007910283732	7.756384530613593
4	1.7080482334134617	4.11181083209103
5	0.808559075502765	2.4330709022145283
6	0.4233611271038189	1.5287456474512493
7	0.2570966746428214	1.0830973764493916
8	0.17390183733513814	0.8372731454382332
9	0.11457730146692771	0.6206033325940733
>10	0.5921925712660622	6.586523940659628
>50	0.06153797747692786	2.6285051688460164
>100	0.049143192413486206	5.546010567853092
>500	0.004120700092881219	1.571281883123857
>1k	0.0010683296537099458	1.3466162207563492
>5k	1.5261852195856367E-4	0.5774623954638269
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6287	0.5760834207644765	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4093	0.37504524275314177	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2226	0.2039703665693852	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.16896736565765783	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1805	0.16539376085253382	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1754	0.1607205853381409	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1576	0.1444102864839852	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1363	0.12489290639446182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.832617848781538E-4
2	0.0	0.0	0.0	0.0	1.832617848781538E-4
3	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
4	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
5	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
6	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
7	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
8	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
9	0.0	0.0	0.0	9.16308924390769E-5	1.832617848781538E-4
10	9.16308924390769E-5	0.0	0.0	2.7489267731723073E-4	1.832617848781538E-4
11	9.16308924390769E-5	0.0	0.0	2.7489267731723073E-4	1.832617848781538E-4
12	1.832617848781538E-4	0.0	0.0	2.7489267731723073E-4	2.7489267731723073E-4
13	1.832617848781538E-4	0.0	0.0	2.7489267731723073E-4	2.7489267731723073E-4
14	1.832617848781538E-4	0.0	0.0	3.665235697563076E-4	2.7489267731723073E-4
15	2.7489267731723073E-4	0.0	0.0	4.5815446219538456E-4	3.665235697563076E-4
16	3.665235697563076E-4	0.0	0.0	5.497853546344615E-4	3.665235697563076E-4
17	3.665235697563076E-4	0.0	0.0	5.497853546344615E-4	3.665235697563076E-4
18	3.665235697563076E-4	0.0	0.0	5.497853546344615E-4	3.665235697563076E-4
19	3.665235697563076E-4	0.0	0.0	5.497853546344615E-4	3.665235697563076E-4
20	3.665235697563076E-4	0.0	0.0	7.330471395126152E-4	5.497853546344615E-4
21	3.665235697563076E-4	0.0	0.0	0.001007939816829846	5.497853546344615E-4
22	3.665235697563076E-4	0.0	0.0	0.0013744633865861537	6.414162470735384E-4
23	3.665235697563076E-4	0.0	0.0	0.003207081235367692	6.414162470735384E-4
24	5.497853546344615E-4	0.0	0.0	0.005314591761466461	6.414162470735384E-4
25	6.414162470735384E-4	0.0	0.0	0.006414162470735384	6.414162470735384E-4
26	6.414162470735384E-4	0.0	0.0	0.008430042104395076	6.414162470735384E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAC	40	1.5954155E-4	23.995703	3
TACGGGG	45	0.008852111	17.777039	21
CCGGACT	45	0.008863802	17.772968	4
TAGCGTA	110	1.0635631E-8	17.453823	7
CGTGCGC	95	7.69116E-7	16.842178	10
ATAGCGT	115	1.8444553E-8	16.692665	6
TTAGGAC	450	0.0	16.352629	3
ACGTTTT	285	0.0	16.280025	29
AGCGTAT	130	4.802132E-9	15.999335	8
AATAGCG	120	3.1161107E-8	15.997137	5
GTCCTAA	535	0.0	15.86726	1
GACGTGA	355	0.0	15.773992	7
CGGTAGG	75	6.1940274E-4	14.949156	1
TTAATAC	140	1.2809323E-8	14.854485	3
TCCAATA	185	1.0913936E-11	14.708833	2
GTGCGCC	100	2.3253846E-5	14.400062	11
ACGAACG	100	2.3253846E-5	14.400062	15
AACCGCG	100	2.3263903E-5	14.399402	7
TAGGACC	500	0.0	14.396103	4
TATGGCG	720	0.0	14.222284	16
>>END_MODULE