FastQCFastQC Report
Thu 2 Feb 2017
SRR4063019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091335
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT75770.6942872720108857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT52730.4831696958312526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT30940.283505981206504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.21505770455451353No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18830.1725409704627818No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17370.1591628601666766No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA14690.134605780993004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.10363453934859598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA350.00206437622.8611376
TTATACC953.783316E-818.5287044
ACCGTCG1650.018.4266178
CGTCGTA1850.018.16200410
ATACCGT1950.018.0544366
ATGCCGG450.00884576217.7792559
ACGCGGC550.001365083517.45359621
GTATAAT1700.016.943361
CCGTCGT1850.016.4337969
TACCGTC1950.016.4131247
ACGTTTT3150.016.25308429
TACGACG1900.016.0027965
ATAATAC1001.3124536E-616.0020623
CCGATAA1001.3130812E-616.001339
CGCGGTT2300.015.30421510
TCCGATA951.4305137E-515.1598498
TAGGACG6650.014.9192154
CACGTTT3450.014.83977228
ACGACGG1951.8189894E-1214.77181156
AGGACGT6500.014.7718115
Produced by FastQC (version 0.11.5)