##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091335
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10445097060023	32.0	32.0	32.0	32.0	32.0
2	31.27744643028951	32.0	32.0	32.0	32.0	32.0
3	31.37038031401907	32.0	32.0	32.0	32.0	32.0
4	31.476631831655723	32.0	32.0	32.0	32.0	32.0
5	31.38989769410859	32.0	32.0	32.0	32.0	32.0
6	34.903806805426385	36.0	36.0	36.0	36.0	36.0
7	34.9351015041211	36.0	36.0	36.0	36.0	36.0
8	34.88267580532101	36.0	36.0	36.0	32.0	36.0
9	34.98469214310913	36.0	36.0	36.0	36.0	36.0
10	34.83928766144218	36.0	36.0	36.0	32.0	36.0
11	35.0165073052729	36.0	36.0	36.0	36.0	36.0
12	34.92946528792717	36.0	36.0	36.0	36.0	36.0
13	34.978846092171516	36.0	36.0	36.0	36.0	36.0
14	34.92007495407002	36.0	36.0	36.0	32.0	36.0
15	34.89126070363362	36.0	36.0	36.0	32.0	36.0
16	34.909235019494474	36.0	36.0	36.0	32.0	36.0
17	34.867937892581104	36.0	36.0	36.0	32.0	36.0
18	34.8587748033372	36.0	36.0	36.0	32.0	36.0
19	34.856180732772245	36.0	36.0	36.0	32.0	36.0
20	34.83057997773369	36.0	36.0	36.0	32.0	36.0
21	34.81355495791851	36.0	36.0	36.0	32.0	36.0
22	34.80345173571818	36.0	36.0	36.0	32.0	36.0
23	34.76130702304975	36.0	36.0	36.0	32.0	36.0
24	34.73708806186918	36.0	36.0	36.0	32.0	36.0
25	34.72335625632826	36.0	36.0	36.0	32.0	36.0
26	34.65898463808088	36.0	36.0	36.0	32.0	36.0
27	34.64217861609863	36.0	36.0	36.0	32.0	36.0
28	34.60223029592197	36.0	36.0	36.0	32.0	36.0
29	34.574255384460315	36.0	36.0	36.0	32.0	36.0
30	34.541527578607855	36.0	36.0	36.0	32.0	36.0
31	34.52143475651381	36.0	36.0	36.0	32.0	36.0
32	34.47774331438101	36.0	36.0	36.0	32.0	36.0
33	34.43907141253602	36.0	36.0	36.0	32.0	36.0
34	34.406477387786516	36.0	36.0	36.0	32.0	36.0
35	34.36549730376099	36.0	36.0	36.0	32.0	36.0
36	34.32663572596865	36.0	36.0	36.0	32.0	36.0
37	34.30475885039883	36.0	36.0	36.0	32.0	36.0
38	33.89066510283277	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	13.0
21	28.0
22	95.0
23	282.0
24	723.0
25	1779.0
26	3752.0
27	7434.0
28	12795.0
29	20565.0
30	31313.0
31	44936.0
32	64595.0
33	101842.0
34	243329.0
35	557852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88950470660684	18.95811339843406	12.395072725140025	25.757309169819067
2	14.494082935120748	21.917468055180123	38.93085074702085	24.657598262678277
3	17.720498288793085	26.919781735214208	29.303101247554604	26.056618728438107
4	11.54164394984125	17.49930131444515	37.36826913825727	33.590785597456325
5	12.823675266852556	38.58153285935641	33.898544257344	14.696247616447042
6	31.446588713069247	37.465374913065034	17.8869327693747	13.20110360449102
7	27.9342636965904	32.008649995876596	22.12318913619162	17.93389717134139
8	26.812912921168135	34.28310929066901	20.192429282238777	18.71154850592407
9	27.644644002565748	14.35645560340878	19.172821405662972	38.826078988362504
10	14.965344974196576	28.080100574712645	33.23666212174525	23.71789232934553
11	35.46748600730882	22.063917367064853	23.71179271390399	18.75680391172234
12	24.522326844626566	24.84239443175862	30.288858019374487	20.346420704240323
13	29.66834198481676	21.172692161435304	25.70090760398961	23.458058249758324
14	22.943642419605347	20.819729963759983	27.151791154869954	29.084836461764716
15	23.79974984766364	28.672222553111553	24.337348293603707	23.1906793056211
16	23.382004609033892	26.880563713250282	26.357992733670226	23.379438944045596
17	22.34455964483866	26.755762437748267	27.219323122597554	23.680354794815525
18	22.95509628116023	25.63099323305859	29.1621729349833	22.251737550797877
19	24.372717818085192	25.610834436721998	27.898766190033307	22.117681555159507
20	24.803016488979093	24.911599096519403	28.01367132915191	22.271713085349596
21	25.028061960809467	25.069937278654127	26.720576175051658	23.18142458548475
22	24.33973069680712	25.898738700765577	27.06290918920405	22.698621413223254
23	22.751829873952197	25.802139037433154	27.732898879534368	23.71313220908028
24	23.33857156601777	26.08988074239349	27.856066194156696	22.715481497432044
25	23.44358056875295	25.608818557088338	27.7285159918815	23.219084882277212
26	22.579409622160014	26.43569573045857	28.19528375796616	22.789610889415258
27	23.415746233508717	26.26482818275615	27.324723686790662	22.994701896944473
28	22.52159052903096	26.252525576472852	27.84882735365401	23.37705654084218
29	22.949506796721447	26.26562879409164	27.646139819578774	23.138724589608138
30	22.86786367155823	26.208084593639903	27.9848075980336	22.93924413676827
31	23.343886157779234	25.938414877191697	27.296384703138816	23.421314261890252
32	22.70402763587716	26.55765644829498	27.370422464229588	23.36789345159827
33	22.40109590547357	26.336184581269727	27.892993443809644	23.369726069447054
34	22.95023984386096	26.432671911008075	27.981967040367987	22.635121204762974
35	23.421222630997814	26.33691762840924	27.64824733010487	22.593612410488074
36	22.650515194692737	26.749531537062403	27.71009818250125	22.88985508574361
37	23.271497752752364	26.770515011430955	26.983831729029124	22.974155506787557
38	22.90644027727508	27.0142531853189	27.258449513669035	22.82085702373698
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	908.0
1	638.0
2	368.0
3	368.0
4	1175.0
5	1982.0
6	1982.0
7	2326.0
8	2670.0
9	2600.5
10	2531.0
11	2531.0
12	3180.0
13	3829.0
14	4627.5
15	5426.0
16	5426.0
17	8041.5
18	10657.0
19	10657.0
20	12233.0
21	13809.0
22	13815.5
23	13822.0
24	13822.0
25	15448.5
26	17075.0
27	17075.0
28	21797.5
29	26520.0
30	32441.5
31	38363.0
32	38363.0
33	45424.0
34	52485.0
35	52485.0
36	57123.5
37	61762.0
38	66646.5
39	71531.0
40	71531.0
41	74366.0
42	77201.0
43	82184.5
44	87168.0
45	87168.0
46	92706.5
47	98245.0
48	98245.0
49	98113.0
50	97981.0
51	93834.0
52	89687.0
53	89687.0
54	83562.0
55	77437.0
56	77437.0
57	74104.0
58	70771.0
59	62414.0
60	54057.0
61	54057.0
62	49182.5
63	44308.0
64	36387.0
65	28466.0
66	28466.0
67	23187.0
68	17908.0
69	17908.0
70	14215.5
71	10523.0
72	8106.0
73	5689.0
74	5689.0
75	4311.5
76	2934.0
77	2934.0
78	2627.5
79	2321.0
80	1809.0
81	1297.0
82	1297.0
83	1163.0
84	1029.0
85	1029.0
86	674.0
87	319.0
88	251.0
89	183.0
90	183.0
91	108.0
92	33.0
93	26.0
94	19.0
95	19.0
96	14.0
97	9.0
98	9.0
99	10.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009437981921224923
2	0.0
3	0.0
4	0.0
5	1.8326178487815384E-4
6	1.8326178487815384E-4
7	4.5815446219538456E-4
8	0.0022907723109769225
9	0.0032070812353676916
10	6.414162470735384E-4
11	0.004673175514392922
12	2.7489267731723073E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.7489267731723073E-4
24	0.0
25	0.0
26	0.0
27	9.163089243907692E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1091335.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.22039388928164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14706595538482	44.333121734356126
2	14.667220995364676	17.078627672225462
3	4.712383506095221	8.230704716466533
4	1.8341561044689065	4.271411634264406
5	0.8513742971855682	2.4783673464677056
6	0.466915458012882	1.63104011271026
7	0.2788843727668275	1.1365730622434962
8	0.17930922409016198	0.835156292360856
9	0.12023957328847378	0.6300355786140682
>10	0.619229707111081	6.746453217177067
>50	0.06423357156532893	2.670077425414832
>100	0.05329409872328355	6.004522051456368
>500	0.004111709291924694	1.502327116766347
>1k	0.0012651413205922136	1.2684660591830583
>5k	3.162853301480534E-4	1.1831159802934093
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7577	0.6942872720108857	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5273	0.4831696958312526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3094	0.283505981206504	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2347	0.21505770455451353	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1883	0.1725409704627818	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1737	0.1591628601666766	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1469	0.134605780993004	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1131	0.10363453934859598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.16308924390769E-5
2	0.0	0.0	0.0	0.0	9.16308924390769E-5
3	0.0	0.0	0.0	9.16308924390769E-5	9.16308924390769E-5
4	0.0	0.0	0.0	9.16308924390769E-5	9.16308924390769E-5
5	0.0	0.0	0.0	9.16308924390769E-5	9.16308924390769E-5
6	0.0	0.0	0.0	9.16308924390769E-5	9.16308924390769E-5
7	0.0	0.0	0.0	9.16308924390769E-5	9.16308924390769E-5
8	0.0	0.0	0.0	1.832617848781538E-4	9.16308924390769E-5
9	0.0	0.0	0.0	1.832617848781538E-4	9.16308924390769E-5
10	0.0	0.0	0.0	2.7489267731723073E-4	9.16308924390769E-5
11	0.0	0.0	0.0	2.7489267731723073E-4	9.16308924390769E-5
12	1.832617848781538E-4	0.0	0.0	2.7489267731723073E-4	9.16308924390769E-5
13	1.832617848781538E-4	0.0	0.0	2.7489267731723073E-4	9.16308924390769E-5
14	1.832617848781538E-4	0.0	0.0	3.665235697563076E-4	9.16308924390769E-5
15	1.832617848781538E-4	0.0	0.0	4.5815446219538456E-4	1.832617848781538E-4
16	1.832617848781538E-4	0.0	0.0	6.414162470735384E-4	1.832617848781538E-4
17	1.832617848781538E-4	0.0	0.0	7.330471395126152E-4	1.832617848781538E-4
18	1.832617848781538E-4	0.0	0.0	8.246780319516922E-4	1.832617848781538E-4
19	1.832617848781538E-4	0.0	0.0	8.246780319516922E-4	1.832617848781538E-4
20	1.832617848781538E-4	0.0	0.0	8.246780319516922E-4	1.832617848781538E-4
21	1.832617848781538E-4	0.0	0.0	0.001099570709268923	2.7489267731723073E-4
22	1.832617848781538E-4	0.0	0.0	0.0015577251714643074	2.7489267731723073E-4
23	1.832617848781538E-4	0.0	0.0	0.003390343020245846	2.7489267731723073E-4
24	1.832617848781538E-4	0.0	0.0	0.005314591761466461	3.665235697563076E-4
25	2.7489267731723073E-4	0.0	0.0	0.00650579336317446	3.665235697563076E-4
26	2.7489267731723073E-4	0.0	0.0	0.008430042104395076	4.5815446219538456E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	35	0.002064376	22.861137	6
TTATACC	95	3.783316E-8	18.528704	4
ACCGTCG	165	0.0	18.426617	8
CGTCGTA	185	0.0	18.162004	10
ATACCGT	195	0.0	18.054436	6
ATGCCGG	45	0.008845762	17.779255	9
ACGCGGC	55	0.0013650835	17.453596	21
GTATAAT	170	0.0	16.94336	1
CCGTCGT	185	0.0	16.433796	9
TACCGTC	195	0.0	16.413124	7
ACGTTTT	315	0.0	16.253084	29
TACGACG	190	0.0	16.002796	5
ATAATAC	100	1.3124536E-6	16.002062	3
CCGATAA	100	1.3130812E-6	16.00133	9
CGCGGTT	230	0.0	15.304215	10
TCCGATA	95	1.4305137E-5	15.159849	8
TAGGACG	665	0.0	14.919215	4
CACGTTT	345	0.0	14.839772	28
ACGACGG	195	1.8189894E-12	14.7718115	6
AGGACGT	650	0.0	14.771811	5
>>END_MODULE