FastQCFastQC Report
Thu 2 Feb 2017
SRR4063018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518945
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37090.2441826399244212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24920.16406123987372814No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23100.15207923920879293No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22530.1483266346049396No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22380.1473391070776098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22290.1467465905612119No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22240.14641741471876862No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21920.14431068932713165No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20960.1379905131522208No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20600.1356204470866292No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19750.13002445776509355No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18610.12251924855738686No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17660.11626490755096465No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17600.11586989654003273No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16890.11119559957733821No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16620.10941805002814453No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16370.10777217081592817No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16160.1063896322776664No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15780.10388789587509752No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15640.10296620351625636No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15570.10250535733683577No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15480.10191284082043787No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15200.10006945610275553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA400.004484185519.9999612
TACACCG450.00886173117.774235
TCTAGCG4300.017.11567928
ATACCGT4250.016.9377986
CTAGCGG4350.016.91950629
AAGACGG5850.016.4069825
ACCGTCG4200.016.3793018
ATCGTTT4100.015.99996829
CAAGACG5900.015.7261924
GCGCAAG5900.015.469821
TACCGTC4600.015.3028057
GTATTAC1052.1680953E-615.2501151
TAGGACG1901.8189894E-1215.1553694
TCTAGAT3500.015.09065152
TAGCGGC4900.015.02037830
CACGACT756.2538893E-414.9308454
CATCGTT4400.014.9085728
TTAGCCG1401.27929525E-814.85711332
CGTCGTA4650.014.79566910
CCGTCGT4550.014.7691999
Produced by FastQC (version 0.11.5)