##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063018_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1518945
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22962516746821	32.0	32.0	32.0	32.0	32.0
2	30.88166786815849	32.0	32.0	32.0	32.0	32.0
3	30.936859464957585	32.0	32.0	32.0	32.0	32.0
4	30.99024717814009	32.0	32.0	32.0	32.0	32.0
5	30.911816425216188	32.0	32.0	32.0	32.0	32.0
6	34.511466182119825	36.0	36.0	36.0	32.0	36.0
7	34.4568118002956	36.0	36.0	36.0	32.0	36.0
8	34.45457669632541	36.0	36.0	36.0	32.0	36.0
9	34.573289355440785	36.0	36.0	36.0	32.0	36.0
10	34.31044047019477	36.0	36.0	36.0	32.0	36.0
11	34.55863181352847	36.0	36.0	36.0	32.0	36.0
12	34.38828002330565	36.0	36.0	36.0	32.0	36.0
13	34.48887813581137	36.0	36.0	36.0	32.0	36.0
14	34.36805150943582	36.0	36.0	36.0	32.0	36.0
15	34.3250887951835	36.0	36.0	36.0	32.0	36.0
16	34.33776535687599	36.0	36.0	36.0	32.0	36.0
17	34.2588112143626	36.0	36.0	36.0	32.0	36.0
18	34.29585008015432	36.0	36.0	36.0	32.0	36.0
19	34.274687365243636	36.0	36.0	36.0	32.0	36.0
20	34.264319642910046	36.0	36.0	36.0	32.0	36.0
21	34.23446866081392	36.0	36.0	36.0	32.0	36.0
22	34.21389187890279	36.0	36.0	36.0	32.0	36.0
23	34.16375247293352	36.0	36.0	36.0	32.0	36.0
24	34.14917722498181	36.0	36.0	36.0	32.0	36.0
25	34.13155380872909	36.0	36.0	36.0	32.0	36.0
26	34.08019184368098	36.0	36.0	36.0	32.0	36.0
27	34.0871558878037	36.0	36.0	36.0	32.0	36.0
28	34.06485159107143	36.0	36.0	36.0	32.0	36.0
29	34.02770607230676	36.0	36.0	36.0	32.0	36.0
30	34.004853368620985	36.0	36.0	36.0	32.0	36.0
31	34.02091319962211	36.0	36.0	36.0	32.0	36.0
32	33.9812863533571	36.0	36.0	36.0	32.0	36.0
33	33.94544766268693	36.0	36.0	36.0	32.0	36.0
34	33.945697177975504	36.0	36.0	36.0	32.0	36.0
35	33.91275984318063	36.0	36.0	36.0	32.0	36.0
36	33.86087119678461	36.0	36.0	36.0	32.0	36.0
37	33.87283476360237	36.0	36.0	36.0	32.0	36.0
38	33.20126930204846	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	5.0
10	8.0
11	14.0
12	8.0
13	12.0
14	238.0
15	485.0
16	638.0
17	766.0
18	934.0
19	1212.0
20	1525.0
21	2119.0
22	2930.0
23	4309.0
24	6157.0
25	9099.0
26	12939.0
27	19094.0
28	27112.0
29	37781.0
30	52362.0
31	73280.0
32	102856.0
33	158554.0
34	353126.0
35	651380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.090464228580615	17.711464954298243	11.502064109671027	25.69600670745011
2	16.706232783140354	19.971293706616148	37.74311095068024	25.57936255956325
3	18.720633783527525	24.278223172710227	28.66959187543457	28.331551168327678
4	12.33549783692288	15.548149616071093	36.66269230186826	35.45366024513777
5	14.562814692312553	36.604641119093266	33.57905228895864	15.253491899635534
6	33.49726355041249	36.146678701383	17.08725630193181	13.268801446272693
7	30.01940822044789	30.580192554827562	21.284063138601557	18.116336086122992
8	27.979588437485802	32.564026431538245	19.865656578291617	19.590728552684332
9	27.463194776364077	14.411610186564069	18.98880931595808	39.136385721113776
10	15.93972379440676	26.09074348509916	31.433544818396502	26.53598790209758
11	37.40157480314961	21.311128960050564	22.665446501461563	18.621849735338266
12	25.16567801910581	23.879821714255616	28.487467986911668	22.467032279726908
13	28.900929516239373	19.381569148778567	25.774931571881293	25.942569763100764
14	23.732875607464514	20.35708148402733	25.03132380352288	30.878719104985276
15	25.01949127765325	27.304777733585844	22.49589761284528	25.179833375915628
16	25.865643742391114	25.831010750054485	23.759286217978474	24.54405928957593
17	23.836480389393863	25.964068512670313	25.33114545114535	24.868305646790475
18	25.017167424359638	24.60850701016878	26.505581470374263	23.868744095097327
19	25.471180093307684	25.003456571633993	25.69858958709441	23.826773747963912
20	25.59799744677097	24.350878717670675	25.054761429781923	24.996362405776434
21	27.16702000184349	24.235742596421	24.480531451219996	24.11670595051552
22	25.68806070972865	24.33834112220295	25.14422247592561	24.82937569214279
23	23.955277880380642	24.05127465678519	25.906553919835453	26.08689354299872
24	24.567751094161423	25.33331840871036	25.37749968230306	24.721430814825155
25	24.74786217014011	24.65831266341962	25.458858915410616	25.134966251029656
26	24.335245956691395	25.73330032225311	25.99069456278536	23.94075915827013
27	25.1491293217875	24.88668805174557	24.91789657206666	25.04628605440027
28	24.00330508865143	24.97606773456583	26.04021410653973	24.98041307024301
29	24.203073077943618	25.00534960755279	25.902305960680877	24.889271353822714
30	24.117489824913456	25.27246584054576	26.286353467561526	24.323690866979263
31	24.958340548160038	25.046366920518835	24.790583482512908	25.204709048808223
32	24.356863274396005	25.189732987895407	24.691064591844462	25.76233914586413
33	23.93309042642146	24.685842881278038	25.660411396697185	25.72065529560332
34	25.020098487527086	24.883409019529605	25.745026735432795	24.351465757510514
35	25.96884728638478	24.60446746980336	25.62230363623913	23.804381607572733
36	24.207107978698186	25.564084533008856	25.036082447074687	25.19272504121827
37	25.421185561308608	25.258953073726236	25.0603104539876	24.259550910977556
38	24.35957112712453	25.043568717430446	25.59687661510404	24.999983540340978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	749.0
1	552.5
2	356.0
3	356.0
4	705.5
5	1055.0
6	1055.0
7	1224.5
8	1394.0
9	1318.0
10	1242.0
11	1242.0
12	1633.5
13	2025.0
14	2727.0
15	3429.0
16	3429.0
17	5030.0
18	6631.0
19	6631.0
20	8179.0
21	9727.0
22	10701.5
23	11676.0
24	11676.0
25	14174.0
26	16672.0
27	16672.0
28	21376.0
29	26080.0
30	31713.0
31	37346.0
32	37346.0
33	47783.0
34	58220.0
35	58220.0
36	63631.5
37	69043.0
38	78536.0
39	88029.0
40	88029.0
41	92348.0
42	96667.0
43	109163.0
44	121659.0
45	121659.0
46	122593.0
47	123527.0
48	123527.0
49	131212.5
50	138898.0
51	140199.5
52	141501.0
53	141501.0
54	132154.0
55	122807.0
56	122807.0
57	119514.0
58	116221.0
59	102850.5
60	89480.0
61	89480.0
62	86787.0
63	84094.0
64	69115.5
65	54137.0
66	54137.0
67	45308.0
68	36479.0
69	36479.0
70	29540.0
71	22601.0
72	17514.0
73	12427.0
74	12427.0
75	9299.0
76	6171.0
77	6171.0
78	6411.0
79	6651.0
80	5159.5
81	3668.0
82	3668.0
83	4012.0
84	4356.0
85	4356.0
86	2816.0
87	1276.0
88	1135.0
89	994.0
90	994.0
91	721.5
92	449.0
93	375.5
94	302.0
95	302.0
96	269.0
97	236.0
98	236.0
99	453.0
100	670.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12021501766028395
2	0.053392321644299176
3	0.012706187518310406
4	0.002896747413500818
5	6.583516848865495E-5
6	3.9501101093192975E-4
7	6.583516848865495E-5
8	1.316703369773099E-4
9	1.9750550546596487E-4
10	2.633406739546198E-4
11	0.0016458792122163736
12	0.002304230897102923
13	0.013035363360753682
14	0.008690242240502454
15	0.02086974841090362
16	0.010665297295162102
17	0.01823634167135742
18	0.005266813479092396
19	0.006517681680376841
20	0.005530154153047017
21	0.005595989321535671
22	0.007307703702240699
23	0.009480264262366313
24	0.0132987040347083
25	0.015273759089367948
26	0.017314649312516254
27	0.008492736735036489
28	0.004937637636649122
29	0.009348593925389004
30	0.002567571571057543
31	0.005398483816069706
32	0.006583516848865495
33	0.007834385050149939
34	0.011455319317025963
35	0.015339594257856606
36	0.013232868866219646
37	0.009019418082945728
38	0.005266813479092396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1518945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.26050721405099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69117755700051	42.16961866827894
2	11.931017854584407	12.47042089320959
3	3.3903796463282996	5.315488798959352
4	1.3672585354737459	2.8581449822639398
5	0.6864483606999998	1.793706975321791
6	0.40269592124788356	1.262705585846636
7	0.2616359931121716	0.9571260793845827
8	0.18030629509462154	0.7538318748425019
9	0.14287654921804233	0.6720120838015391
>10	0.7492770638785109	7.100455750424752
>50	0.08194160509524716	3.0342912359602834
>100	0.09340140379085145	10.801491454637016
>500	0.015019897793097384	5.499909296621693
>1k	0.006563316682698017	5.310796320447378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3709	0.2441826399244212	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2492	0.16406123987372814	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2310	0.15207923920879293	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2253	0.1483266346049396	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2238	0.1473391070776098	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2229	0.1467465905612119	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2224	0.14641741471876862	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2192	0.14431068932713165	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2096	0.1379905131522208	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2060	0.1356204470866292	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1975	0.13002445776509355	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1861	0.12251924855738686	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1766	0.11626490755096465	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1760	0.11586989654003273	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1689	0.11119559957733821	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1662	0.10941805002814453	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1637	0.10777217081592817	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1616	0.1063896322776664	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1578	0.10388789587509752	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1564	0.10296620351625636	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1557	0.10250535733683577	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1548	0.10191284082043787	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1520	0.10006945610275553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.583516848865495E-5
7	0.0	0.0	0.0	0.0	6.583516848865495E-5
8	0.0	0.0	0.0	0.0	6.583516848865495E-5
9	0.0	0.0	0.0	0.0	6.583516848865495E-5
10	0.0	0.0	0.0	6.583516848865495E-5	6.583516848865495E-5
11	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	6.583516848865495E-5
12	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	1.316703369773099E-4
13	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	2.633406739546198E-4
14	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	2.633406739546198E-4
15	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
16	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
17	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
18	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
19	6.583516848865495E-5	0.0	0.0	1.9750550546596487E-4	3.2917584244327477E-4
20	6.583516848865495E-5	0.0	0.0	1.9750550546596487E-4	4.6084617942058467E-4
21	6.583516848865495E-5	0.0	0.0	4.6084617942058467E-4	5.266813479092396E-4
22	6.583516848865495E-5	0.0	0.0	6.583516848865495E-4	5.266813479092396E-4
23	6.583516848865495E-5	0.0	0.0	0.0010533626958184792	5.266813479092396E-4
24	6.583516848865495E-5	0.0	0.0	0.0021725605601256135	5.266813479092396E-4
25	6.583516848865495E-5	0.0	0.0	0.002633406739546198	5.266813479092396E-4
26	6.583516848865495E-5	0.0	0.0	0.003357593592921403	5.266813479092396E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	40	0.0044841855	19.99996	12
TACACCG	45	0.008861731	17.77423	5
TCTAGCG	430	0.0	17.115679	28
ATACCGT	425	0.0	16.937798	6
CTAGCGG	435	0.0	16.919506	29
AAGACGG	585	0.0	16.406982	5
ACCGTCG	420	0.0	16.379301	8
ATCGTTT	410	0.0	15.999968	29
CAAGACG	590	0.0	15.726192	4
GCGCAAG	590	0.0	15.46982	1
TACCGTC	460	0.0	15.302805	7
GTATTAC	105	2.1680953E-6	15.250115	1
TAGGACG	190	1.8189894E-12	15.155369	4
TCTAGAT	350	0.0	15.0906515	2
TAGCGGC	490	0.0	15.020378	30
CACGACT	75	6.2538893E-4	14.930845	4
CATCGTT	440	0.0	14.90857	28
TTAGCCG	140	1.27929525E-8	14.857113	32
CGTCGTA	465	0.0	14.795669	10
CCGTCGT	455	0.0	14.769199	9
>>END_MODULE