FastQCFastQC Report
Thu 2 Feb 2017
SRR4063018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518945
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46170.30396097291211993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31390.2066565938858879No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24180.15918943740556768No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23970.15780689886730592No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22410.14753661258307577No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22360.1472074367406325No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21940.14444235966410895No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21630.14240146944096066No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21400.1408872605657216No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20960.1379905131522208No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20440.13456708439081072No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20090.1322628534937078No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19140.12600851248728556No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18910.12449430361204653No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18380.1210050396821478No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18250.1201491824917953No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17970.11830579777411296No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17270.11369733597990711No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16990.11185395126222476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16520.108759698343258No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT15980.10520459924487063No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15800.10401956621207482No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT15780.10388789587509752No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15390.10132032430403998No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15240.10033279677671016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC450.00885270117.77737624
GCTCTAA652.1007984E-417.2309461
ATTCGTT652.101327E-417.2303832
TAGGACG2200.016.7274444
ACCGTCG5000.015.6811928
TATACTG1652.910383E-1115.5163345
AGGGGCG5100.015.37220232
TTATACT2000.015.2001564
ACTATAC951.4333029E-515.1575513
CTAGCGG4250.015.05848129
TACCGTC5000.015.0411447
CGTCGTA5150.014.91375510
ATACCGT5150.014.9137556
TCTAGCG4400.014.90875328
TAGCGGC4350.014.71231130
AAGACGG5450.014.6800165
CCGTCGT5250.014.6296859
CGCGTAA2850.014.59760310
GTCGTAG5400.014.51914611
GATCACG901.5349925E-414.22190113
Produced by FastQC (version 0.11.5)