##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1518945
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114695397134195	32.0	32.0	32.0	32.0	32.0
2	31.07124352758	32.0	32.0	32.0	32.0	32.0
3	31.230676555108975	32.0	32.0	32.0	32.0	32.0
4	31.366435256049428	32.0	32.0	32.0	32.0	32.0
5	31.236130340466573	32.0	32.0	32.0	32.0	32.0
6	34.76161480501269	36.0	36.0	36.0	32.0	36.0
7	34.78451688507484	36.0	36.0	36.0	32.0	36.0
8	34.72085361879462	36.0	36.0	36.0	32.0	36.0
9	34.88357774639635	36.0	36.0	36.0	32.0	36.0
10	34.66467054435809	36.0	36.0	36.0	32.0	36.0
11	34.89960400146154	36.0	36.0	36.0	36.0	36.0
12	34.76187551227991	36.0	36.0	36.0	32.0	36.0
13	34.853055903933324	36.0	36.0	36.0	32.0	36.0
14	34.78144435776147	36.0	36.0	36.0	32.0	36.0
15	34.74982043457795	36.0	36.0	36.0	32.0	36.0
16	34.755945080302446	36.0	36.0	36.0	32.0	36.0
17	34.70973603389194	36.0	36.0	36.0	32.0	36.0
18	34.722223648650875	36.0	36.0	36.0	32.0	36.0
19	34.723769458406984	36.0	36.0	36.0	32.0	36.0
20	34.70192139939234	36.0	36.0	36.0	32.0	36.0
21	34.69446424985763	36.0	36.0	36.0	32.0	36.0
22	34.67998051279013	36.0	36.0	36.0	32.0	36.0
23	34.61481225455826	36.0	36.0	36.0	32.0	36.0
24	34.58583688020303	36.0	36.0	36.0	32.0	36.0
25	34.569057470810336	36.0	36.0	36.0	32.0	36.0
26	34.492200178413306	36.0	36.0	36.0	32.0	36.0
27	34.482567834911734	36.0	36.0	36.0	32.0	36.0
28	34.44138069515354	36.0	36.0	36.0	32.0	36.0
29	34.40438133046292	36.0	36.0	36.0	32.0	36.0
30	34.387279987096306	36.0	36.0	36.0	32.0	36.0
31	34.38163725480514	36.0	36.0	36.0	32.0	36.0
32	34.35481271540444	36.0	36.0	36.0	32.0	36.0
33	34.311436556294005	36.0	36.0	36.0	32.0	36.0
34	34.304831972191224	36.0	36.0	36.0	32.0	36.0
35	34.2746498391976	36.0	36.0	36.0	32.0	36.0
36	34.22613787859336	36.0	36.0	36.0	32.0	36.0
37	34.23361543703031	36.0	36.0	36.0	32.0	36.0
38	33.75030234801128	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	22.0
22	114.0
23	374.0
24	1035.0
25	2564.0
26	5548.0
27	10996.0
28	18959.0
29	30548.0
30	47404.0
31	70081.0
32	102486.0
33	166676.0
34	384238.0
35	677888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11678851339963	19.166697391707398	12.35462666441935	26.361887430473622
2	15.613731899443364	21.20208434143435	36.86440259522234	26.31978116389994
3	17.5610045130008	25.31750655882866	29.050294776966908	28.07119415120363
4	11.898669008646143	16.58008233357012	36.83633948081001	34.684909176973726
5	14.124088840668254	37.0163745338867	33.11623074978406	15.743305875660985
6	33.51909847834975	36.70559066403413	16.603256343391422	13.172054514224692
7	29.599530726301122	30.91320740804625	21.21323841645144	18.274023449201188
8	27.668200434915143	32.9274081467256	19.67355688296332	19.730834535395942
9	27.397747293407527	14.211421017009856	18.660213683204248	39.730618006378364
10	15.650501109663454	26.184765713209767	31.44202263568607	26.722710541440716
11	37.448514152682534	21.24996049679234	22.59037017139125	18.71115517913388
12	24.945422321941148	23.923391110619537	28.46880974576941	22.662376821669906
13	28.951777650642583	19.287623037862513	25.700635244377175	26.059964067117726
14	23.528896701328883	20.24872526655014	25.12125192156398	31.101126110556997
15	24.981023012683146	27.42719453304761	22.375530384576138	25.216252069693105
16	25.804901299850425	25.879953441338195	23.66519108011882	24.649954178692564
17	23.875650533758627	26.12694995539667	25.114734239883603	24.882665270961095
18	25.00979298131269	24.523995273034902	26.535588846205755	23.930622899446654
19	25.41968274032305	24.994848398065763	25.681970051581853	23.90349881002933
20	25.580188881098394	24.209500673164598	25.128625460434712	25.0816849853023
21	27.100454591838414	24.28369690805131	24.44176714759257	24.1740813525177
22	25.520213042605228	24.317338679149014	25.27714960054512	24.88529867770064
23	23.802078155664827	23.89016572708785	26.02329382998572	26.2844622872616
24	24.587723716131922	25.17049662759349	25.466491545118487	24.775288111156097
25	24.719805338445656	24.520982998714896	25.596467019192282	25.162744643647166
26	24.421226575024114	25.525414020915832	26.015161839302937	24.03819756475712
27	25.229699054079674	24.621645037605074	25.013627888849093	25.135028019466155
28	24.080002896747413	24.81814680584221	26.245519093844745	24.85633120356563
29	24.348544549012637	24.716694811201194	26.048145258715756	24.886615381070413
30	24.188170078574274	24.91768958059706	26.418270575958974	24.475869764869696
31	25.033361971631624	24.846324257955356	24.893330568256257	25.22698320215676
32	24.449469862305744	25.02243333366251	24.85415864300551	25.67393816102624
33	24.062688247434895	24.47172214925491	25.85017890707037	25.615410696239827
34	24.996757617951936	24.549210142566057	25.971578957763448	24.48245328171856
35	25.978623320791733	24.43959458703245	25.61139475096202	23.970387341213804
36	24.380540440898123	25.36016774800931	25.19380227723848	25.06548953385409
37	25.59513346434532	25.0277001471416	25.045278137128076	24.331888251385006
38	24.417226704868643	24.638433016622074	25.809707270314114	25.134633008195166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	402.0
1	312.5
2	223.0
3	223.0
4	656.5
5	1090.0
6	1090.0
7	1204.5
8	1319.0
9	1283.0
10	1247.0
11	1247.0
12	1685.5
13	2124.0
14	2879.5
15	3635.0
16	3635.0
17	5455.5
18	7276.0
19	7276.0
20	8771.5
21	10267.0
22	11278.0
23	12289.0
24	12289.0
25	14428.0
26	16567.0
27	16567.0
28	21316.0
29	26065.0
30	31614.5
31	37164.0
32	37164.0
33	48127.0
34	59090.0
35	59090.0
36	64353.5
37	69617.0
38	78921.0
39	88225.0
40	88225.0
41	92047.0
42	95869.0
43	108525.0
44	121181.0
45	121181.0
46	121942.0
47	122703.0
48	122703.0
49	130724.0
50	138745.0
51	139231.0
52	139717.0
53	139717.0
54	131642.0
55	123567.0
56	123567.0
57	120479.0
58	117391.0
59	103934.5
60	90478.0
61	90478.0
62	87158.0
63	83838.0
64	69008.0
65	54178.0
66	54178.0
67	45538.5
68	36899.0
69	36899.0
70	30091.0
71	23283.0
72	17963.0
73	12643.0
74	12643.0
75	9282.5
76	5922.0
77	5922.0
78	6296.0
79	6670.0
80	5213.5
81	3757.0
82	3757.0
83	3668.5
84	3580.0
85	3580.0
86	2297.0
87	1014.0
88	840.0
89	666.0
90	666.0
91	397.0
92	128.0
93	92.5
94	57.0
95	57.0
96	41.5
97	26.0
98	26.0
99	29.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0063860113433995315
2	0.0
3	0.0
4	1.316703369773099E-4
5	6.583516848865495E-5
6	1.316703369773099E-4
7	1.316703369773099E-4
8	0.0018433847176823387
9	0.004015945277807952
10	0.0010533626958184792
11	0.005332648647581051
12	5.925165163978946E-4
13	1.316703369773099E-4
14	0.0
15	0.0
16	6.583516848865495E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.316703369773099E-4
24	0.0
25	6.583516848865495E-5
26	0.0
27	6.583516848865495E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.583516848865495E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1518945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.49941159290452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48418157621367	40.13904400542373
2	12.633449133458043	12.759634952570412
3	3.6011756727618494	5.455717575514663
4	1.4535501371468706	2.9361370658681056
5	0.7214321117415913	1.8215948573588454
6	0.44246336064390585	1.340648361836181
7	0.2914014986365701	1.030092295290615
8	0.21374743751859268	0.8635295859344043
9	0.16365861624202174	0.7438197440097958
>10	0.7864326450511994	7.214609390852927
>50	0.08789055682148601	3.146364305440859
>100	0.09717486492969131	10.953653819151972
>500	0.01584653099981267	5.578271444416573
>1k	0.00759585783462095	6.016882596330958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4617	0.30396097291211993	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3139	0.2066565938858879	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2418	0.15918943740556768	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2397	0.15780689886730592	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2241	0.14753661258307577	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2236	0.1472074367406325	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2194	0.14444235966410895	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2163	0.14240146944096066	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2140	0.1408872605657216	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2096	0.1379905131522208	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2044	0.13456708439081072	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2009	0.1322628534937078	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1914	0.12600851248728556	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1891	0.12449430361204653	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1838	0.1210050396821478	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1825	0.1201491824917953	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1797	0.11830579777411296	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1727	0.11369733597990711	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1699	0.11185395126222476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1652	0.108759698343258	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1598	0.10520459924487063	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1580	0.10401956621207482	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1578	0.10388789587509752	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1539	0.10132032430403998	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1524	0.10033279677671016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.316703369773099E-4
2	0.0	0.0	0.0	0.0	1.316703369773099E-4
3	0.0	0.0	0.0	0.0	1.316703369773099E-4
4	0.0	0.0	0.0	0.0	1.316703369773099E-4
5	0.0	0.0	0.0	0.0	1.316703369773099E-4
6	0.0	0.0	0.0	0.0	1.316703369773099E-4
7	0.0	0.0	0.0	0.0	1.316703369773099E-4
8	0.0	0.0	0.0	0.0	1.316703369773099E-4
9	0.0	0.0	0.0	0.0	1.9750550546596487E-4
10	0.0	0.0	0.0	6.583516848865495E-5	1.9750550546596487E-4
11	0.0	0.0	0.0	6.583516848865495E-5	1.9750550546596487E-4
12	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
13	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.2917584244327477E-4
14	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.9501101093192975E-4
15	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	3.9501101093192975E-4
16	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	4.6084617942058467E-4
17	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	4.6084617942058467E-4
18	6.583516848865495E-5	0.0	0.0	6.583516848865495E-5	5.266813479092396E-4
19	6.583516848865495E-5	0.0	0.0	1.316703369773099E-4	5.266813479092396E-4
20	6.583516848865495E-5	0.0	0.0	1.9750550546596487E-4	7.241868533752045E-4
21	6.583516848865495E-5	0.0	0.0	3.9501101093192975E-4	7.241868533752045E-4
22	6.583516848865495E-5	0.0	0.0	5.925165163978946E-4	7.241868533752045E-4
23	6.583516848865495E-5	0.0	0.0	9.875275273298243E-4	7.241868533752045E-4
24	6.583516848865495E-5	0.0	0.0	0.0020408902231483037	7.241868533752045E-4
25	6.583516848865495E-5	0.0	0.0	0.0024359012340802334	7.241868533752045E-4
26	6.583516848865495E-5	0.0	0.0	0.003094252918966783	7.241868533752045E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	45	0.008852701	17.777376	24
GCTCTAA	65	2.1007984E-4	17.230946	1
ATTCGTT	65	2.101327E-4	17.23038	32
TAGGACG	220	0.0	16.727444	4
ACCGTCG	500	0.0	15.681192	8
TATACTG	165	2.910383E-11	15.516334	5
AGGGGCG	510	0.0	15.372202	32
TTATACT	200	0.0	15.200156	4
ACTATAC	95	1.4333029E-5	15.157551	3
CTAGCGG	425	0.0	15.058481	29
TACCGTC	500	0.0	15.041144	7
CGTCGTA	515	0.0	14.913755	10
ATACCGT	515	0.0	14.913755	6
TCTAGCG	440	0.0	14.908753	28
TAGCGGC	435	0.0	14.712311	30
AAGACGG	545	0.0	14.680016	5
CCGTCGT	525	0.0	14.629685	9
CGCGTAA	285	0.0	14.597603	10
GTCGTAG	540	0.0	14.519146	11
GATCACG	90	1.5349925E-4	14.221901	13
>>END_MODULE