FastQCFastQC Report
Thu 2 Feb 2017
SRR4063017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382905
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT56310.23630820364219304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35490.1489358577030977No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31150.13072279423644667No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30010.12593871765764897No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25390.10655061783831081No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25110.10537558148562365No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24640.10340319903647019No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24570.1031094399482984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG7000.019.6565528
CTAGCGG7100.019.38091729
TAGCGGC7350.018.93930
CAAGACG7850.017.7274844
AAGACGG7850.017.5248245
TAGATCG550.001367233517.4507225
CGCAAGA7750.016.3136372
AGCGGCG8900.016.00018931
GCGCAAG7950.015.9165961
GACGGAC8650.015.7207497
ACACCGT1652.910383E-1115.5117546
CGGTCCA9350.015.05900210
AGACGGA9150.015.0349586
CGCCGGT9050.014.67235857
GTATCAA42100.014.647681
GCCGGTC9300.014.6222948
CCGGTCC9550.014.5757839
CGACGCT1103.5456378E-614.54562615
TCGACGC1103.5456378E-614.54562614
ACCGTCG5100.014.4300298
Produced by FastQC (version 0.11.5)