##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063017_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2382905
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25924575255833	32.0	32.0	32.0	32.0	32.0
2	30.89666688348885	32.0	32.0	32.0	32.0	32.0
3	30.941988455267836	32.0	32.0	32.0	32.0	32.0
4	31.004049259202528	32.0	32.0	32.0	32.0	32.0
5	30.923797633560717	32.0	32.0	32.0	32.0	32.0
6	34.57374968788097	36.0	36.0	36.0	32.0	36.0
7	34.509875970716415	36.0	36.0	36.0	32.0	36.0
8	34.497781069744704	36.0	36.0	36.0	32.0	36.0
9	34.63062018838351	36.0	36.0	36.0	32.0	36.0
10	34.36583371976642	36.0	36.0	36.0	32.0	36.0
11	34.61402615714852	36.0	36.0	36.0	32.0	36.0
12	34.43845684154425	36.0	36.0	36.0	32.0	36.0
13	34.52329866276666	36.0	36.0	36.0	32.0	36.0
14	34.42672452321851	36.0	36.0	36.0	32.0	36.0
15	34.37504852270653	36.0	36.0	36.0	32.0	36.0
16	34.38918169209431	36.0	36.0	36.0	32.0	36.0
17	34.319624995541155	36.0	36.0	36.0	32.0	36.0
18	34.34736508589306	36.0	36.0	36.0	32.0	36.0
19	34.32869669583974	36.0	36.0	36.0	32.0	36.0
20	34.31479265854073	36.0	36.0	36.0	32.0	36.0
21	34.3033180089009	36.0	36.0	36.0	32.0	36.0
22	34.27095876671542	36.0	36.0	36.0	32.0	36.0
23	34.21919254019778	36.0	36.0	36.0	32.0	36.0
24	34.20484282839643	36.0	36.0	36.0	32.0	36.0
25	34.18428892465289	36.0	36.0	36.0	32.0	36.0
26	34.14152011935012	36.0	36.0	36.0	32.0	36.0
27	34.14275180924124	36.0	36.0	36.0	32.0	36.0
28	34.124729689181905	36.0	36.0	36.0	32.0	36.0
29	34.09404319517564	36.0	36.0	36.0	32.0	36.0
30	34.06688264953911	36.0	36.0	36.0	32.0	36.0
31	34.08597699027028	36.0	36.0	36.0	32.0	36.0
32	34.035808813192304	36.0	36.0	36.0	32.0	36.0
33	34.00571151598574	36.0	36.0	36.0	32.0	36.0
34	34.01465312297385	36.0	36.0	36.0	32.0	36.0
35	33.97443288758889	36.0	36.0	36.0	32.0	36.0
36	33.930701391788595	36.0	36.0	36.0	32.0	36.0
37	33.93752709403019	36.0	36.0	36.0	32.0	36.0
38	33.348838917204	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	25.0
11	24.0
12	9.0
13	12.0
14	225.0
15	615.0
16	752.0
17	974.0
18	1126.0
19	1485.0
20	1997.0
21	2818.0
22	4337.0
23	6590.0
24	10043.0
25	15046.0
26	21670.0
27	31885.0
28	44741.0
29	60417.0
30	81398.0
31	109837.0
32	150233.0
33	226498.0
34	510138.0
35	1099999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.702720599109945	16.993534627640265	11.378559568146066	25.92518520510373
2	17.01814001354528	19.219647293609743	37.20229035358394	26.55992233926104
3	19.38345016422918	22.970050943362967	28.079832253721282	29.566666638686566
4	12.861383869930766	15.010529390386488	35.39746874965902	36.73061799002373
5	15.088637831481739	35.92418310637589	33.25411609975916	15.733062962383215
6	34.97199834654905	34.854871910008626	16.545000933738162	13.628128809704162
7	31.21484070913444	29.883818280628056	20.215745067470166	18.685595942767335
8	28.524265170898833	32.502399386294265	19.10503205966173	19.868303383145168
9	27.42822515226176	14.489972944765272	18.40859657317146	39.67320532980151
10	16.135513926523952	26.080543069597155	31.121503681020474	26.662439322858418
11	37.78049253376822	21.08926156865148	21.939373744681813	19.19087215289849
12	25.427703557348746	23.373182107752	28.04957273709293	23.149541597806316
13	29.433784875607273	19.104246456040208	25.114634376672296	26.34733429168022
14	23.8184153204991	19.64540078650601	24.782577674812924	31.75360621818197
15	25.367145056034563	26.81935502015011	22.1842628073149	25.629237116500427
16	26.188197807308022	25.664493448868946	23.293679873014913	24.85362887080812
17	24.27875201527178	25.671152137331877	24.973294107326417	25.07680174006992
18	25.28910546122367	24.530454120626278	25.94577512578789	24.23466529236216
19	25.75167853398659	24.818422870534437	25.266815347411836	24.163083248067135
20	25.840076214138225	24.089711091339456	24.90850945961826	25.16170323490406
21	26.94336353899821	24.029829073316165	24.402167891568492	24.624639496117133
22	25.969121082323355	24.1336603537293	24.90936851320714	24.9878500507402
23	24.455664326859516	24.003147722622682	25.5265195630961	26.0146683874217
24	24.935112357192622	24.92487135793971	25.089860571313448	25.05015571355421
25	25.095361374278287	24.3098892095583	25.138425093472616	25.456324322690804
26	24.716157828419558	25.1827712733674	25.606152565009825	24.494918333203216
27	25.50483504280212	24.590959282060883	24.732185088485416	25.172020586651577
28	24.646306440829903	24.54449272132628	25.57345759139628	25.235743246447534
29	24.600404589828177	24.81931958399436	25.565754241058315	25.014521585119155
30	24.609939911987304	24.939882610567736	25.721639490389194	24.72853798705577
31	25.232407396915445	24.808028615350914	24.598231391355547	25.361332596378087
32	24.834047003613048	24.78674877337265	24.58823889845066	25.790965324563647
33	24.461747789913343	24.451255544102686	25.239516987365658	25.847479678618313
34	25.3752779486904	24.518620988368337	25.378719517978844	24.727381544962423
35	25.87492041026958	24.462221058193997	25.26296614818443	24.39989238335199
36	24.660083011306714	25.05495123536925	24.9725191358732	25.31244661745084
37	25.65960306091914	24.821262417199435	24.714575963315305	24.80455855856612
38	24.699238117461018	24.68450743710503	25.329509791495823	25.286744653938126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	196.0
1	279.5
2	363.0
3	363.0
4	795.5
5	1228.0
6	1228.0
7	1465.5
8	1703.0
9	1663.5
10	1624.0
11	1624.0
12	2198.0
13	2772.0
14	3754.0
15	4736.0
16	4736.0
17	7043.0
18	9350.0
19	9350.0
20	11346.0
21	13342.0
22	14601.5
23	15861.0
24	15861.0
25	18749.5
26	21638.0
27	21638.0
28	27811.5
29	33985.0
30	42113.0
31	50241.0
32	50241.0
33	64150.0
34	78059.0
35	78059.0
36	87599.0
37	97139.0
38	111499.5
39	125860.0
40	125860.0
41	135064.0
42	144268.0
43	163696.5
44	183125.0
45	183125.0
46	189477.5
47	195830.0
48	195830.0
49	209510.5
50	223191.0
51	225979.0
52	228767.0
53	228767.0
54	219867.5
55	210968.0
56	210968.0
57	204385.0
58	197802.0
59	176980.5
60	156159.0
61	156159.0
62	146823.0
63	137487.0
64	114274.5
65	91062.0
66	91062.0
67	76086.5
68	61111.0
69	61111.0
70	49224.5
71	37338.0
72	29185.0
73	21032.0
74	21032.0
75	15927.0
76	10822.0
77	10822.0
78	10218.0
79	9614.0
80	7517.0
81	5420.0
82	5420.0
83	5393.0
84	5366.0
85	5366.0
86	3579.5
87	1793.0
88	1546.5
89	1300.0
90	1300.0
91	938.5
92	577.0
93	501.5
94	426.0
95	426.0
96	378.0
97	330.0
98	330.0
99	675.0
100	1020.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11922422421372234
2	0.053044498207020424
3	0.010869086262356241
4	0.0021402447852516154
5	2.5179350414724884E-4
6	4.196558402454147E-4
7	0.0
8	1.678623360981659E-4
9	8.393116804908296E-5
10	2.5179350414724884E-4
11	0.0018045201130552832
12	0.0026438317935461126
13	0.013848642728098685
14	0.009022600565276418
15	0.022493553037154227
16	0.011036948598454407
17	0.019807755659583575
18	0.005203732419043143
19	0.007176114868196592
20	0.006085009683558514
21	0.00541356033916585
22	0.007511839540392924
23	0.009652084325644539
24	0.013638814807975979
25	0.016324612185546632
26	0.017583579706282878
27	0.00822525446881013
28	0.00511980125099406
29	0.01028156808601266
30	0.0028116941296442784
31	0.005371594755141309
32	0.006378768771730305
33	0.008099357716736505
34	0.01137267327065074
35	0.015695128425178513
36	0.01418436740029502
37	0.009316359653448207
38	0.005161766835018601
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2382905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.162339508330035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42725433522124	41.693763216751805
2	12.850115425991637	13.662843979955932
3	3.9316378361617934	6.270451964094881
4	1.6256427203321064	3.456918808701627
5	0.8508844719804198	2.261750459089461
6	0.5145638168539148	1.641324979817399
7	0.34439399926622144	1.2816153499535703
8	0.24503372115500144	1.0421252700025327
9	0.17898393738852472	0.8563684361387794
>10	0.880707488512014	8.399918263517987
>50	0.0660926519709733	2.4807715640930965
>100	0.06744198173071696	7.827497249002866
>500	0.011946741416292678	4.394334753120576
>1k	0.005221754526326325	4.493471846932427
>5k	7.911749282312615E-5	0.23684385882710865
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5631	0.23630820364219304	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3549	0.1489358577030977	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3115	0.13072279423644667	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3001	0.12593871765764897	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2539	0.10655061783831081	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2511	0.10537558148562365	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2464	0.10340319903647019	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2457	0.1031094399482984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.196558402454147E-5
7	0.0	0.0	0.0	4.196558402454147E-5	4.196558402454147E-5
8	0.0	0.0	0.0	4.196558402454147E-5	4.196558402454147E-5
9	0.0	0.0	0.0	4.196558402454147E-5	4.196558402454147E-5
10	0.0	0.0	0.0	8.393116804908294E-5	8.393116804908294E-5
11	0.0	0.0	0.0	1.6786233609816589E-4	8.393116804908294E-5
12	0.0	0.0	0.0	2.0982792012270738E-4	2.9375908817179033E-4
13	0.0	0.0	0.0	2.5179350414724884E-4	3.3572467219633177E-4
14	0.0	0.0	0.0	2.5179350414724884E-4	3.3572467219633177E-4
15	0.0	0.0	0.0	2.9375908817179033E-4	3.7769025622087326E-4
16	0.0	0.0	0.0	2.9375908817179033E-4	5.035870082944977E-4
17	0.0	0.0	0.0	3.3572467219633177E-4	5.455525923190391E-4
18	0.0	0.0	0.0	4.1965584024541476E-4	5.875181763435807E-4
19	0.0	0.0	0.0	5.035870082944977E-4	5.875181763435807E-4
20	0.0	0.0	0.0	6.714493443926635E-4	5.875181763435807E-4
21	0.0	0.0	0.0	8.393116804908295E-4	5.875181763435807E-4
22	0.0	0.0	0.0	0.0010071740165889954	5.875181763435807E-4
23	0.0	0.0	0.0	0.001594692192932576	5.875181763435807E-4
24	0.0	0.0	0.0	0.0028956252976933615	5.875181763435807E-4
25	0.0	0.0	0.0	0.003357246721963318	5.875181763435807E-4
26	0.0	0.0	0.0	0.004070661650380523	5.875181763435807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	700	0.0	19.65655	28
CTAGCGG	710	0.0	19.380917	29
TAGCGGC	735	0.0	18.939	30
CAAGACG	785	0.0	17.727484	4
AAGACGG	785	0.0	17.524824	5
TAGATCG	55	0.0013672335	17.450722	5
CGCAAGA	775	0.0	16.313637	2
AGCGGCG	890	0.0	16.000189	31
GCGCAAG	795	0.0	15.916596	1
GACGGAC	865	0.0	15.720749	7
ACACCGT	165	2.910383E-11	15.511754	6
CGGTCCA	935	0.0	15.059002	10
AGACGGA	915	0.0	15.034958	6
CGCCGGT	905	0.0	14.6723585	7
GTATCAA	4210	0.0	14.64768	1
GCCGGTC	930	0.0	14.622294	8
CCGGTCC	955	0.0	14.575783	9
CGACGCT	110	3.5456378E-6	14.545626	15
TCGACGC	110	3.5456378E-6	14.545626	14
ACCGTCG	510	0.0	14.430029	8
>>END_MODULE