FastQCFastQC Report
Thu 2 Feb 2017
SRR4063017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382905
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61780.2592633781036172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40340.16928916595500032No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32470.13626225132768618No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30860.129505792299735No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA27200.1141463885467528No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26760.11229990284967299No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26180.10986589897624957No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25800.108271206783317No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24400.1023960250198812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5900.018.4399428
CTAGCGG6150.017.69034829
ATCGTTT5050.016.47458829
TAGGACG3500.016.0013754
CGGTCCA7950.015.69847310
TAGCGGC7050.015.65894830
ACCGTCG6150.015.6114248
CGCTTCG5300.015.3956132
TACCGTC6100.015.2147427
ATACCGT6000.015.2016256
CATCGTT5650.015.00824828
TAAACGC5450.014.97187828
AGCGGCG7700.014.75265631
CGTCGTA6350.014.6145110
GTATCAA41650.014.5990931
CGTTTAT5650.014.44189931
CCGTCGT6350.014.3631379
GTAAACG5750.014.19073727
CGCCGGT7600.014.1067717
AAACGCT5700.014.03452629
Produced by FastQC (version 0.11.5)