##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2382905
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205995203333746	32.0	32.0	32.0	32.0	32.0
2	31.274049531978825	32.0	32.0	32.0	32.0	32.0
3	31.36795927659726	32.0	32.0	32.0	32.0	32.0
4	31.468310738363467	32.0	32.0	32.0	32.0	32.0
5	31.388660059884888	32.0	32.0	32.0	32.0	32.0
6	34.91352487824735	36.0	36.0	36.0	36.0	36.0
7	34.943189090626774	36.0	36.0	36.0	36.0	36.0
8	34.878450882431316	36.0	36.0	36.0	32.0	36.0
9	34.99932183616216	36.0	36.0	36.0	36.0	36.0
10	34.83758311808486	36.0	36.0	36.0	32.0	36.0
11	35.015250293234516	36.0	36.0	36.0	36.0	36.0
12	34.915005424051735	36.0	36.0	36.0	32.0	36.0
13	34.964036333802646	36.0	36.0	36.0	36.0	36.0
14	34.90296675696261	36.0	36.0	36.0	32.0	36.0
15	34.87948995029176	36.0	36.0	36.0	32.0	36.0
16	34.892710368227014	36.0	36.0	36.0	32.0	36.0
17	34.85313304558931	36.0	36.0	36.0	32.0	36.0
18	34.858809730140315	36.0	36.0	36.0	32.0	36.0
19	34.84697417647787	36.0	36.0	36.0	32.0	36.0
20	34.835181427711134	36.0	36.0	36.0	32.0	36.0
21	34.830854356342364	36.0	36.0	36.0	32.0	36.0
22	34.81530778608463	36.0	36.0	36.0	32.0	36.0
23	34.75853002952279	36.0	36.0	36.0	32.0	36.0
24	34.73257725339449	36.0	36.0	36.0	32.0	36.0
25	34.71655059685552	36.0	36.0	36.0	32.0	36.0
26	34.651357901385076	36.0	36.0	36.0	32.0	36.0
27	34.63493634870043	36.0	36.0	36.0	32.0	36.0
28	34.60854251428403	36.0	36.0	36.0	32.0	36.0
29	34.57221332784983	36.0	36.0	36.0	32.0	36.0
30	34.5511537388188	36.0	36.0	36.0	32.0	36.0
31	34.54955401075578	36.0	36.0	36.0	32.0	36.0
32	34.52091124069151	36.0	36.0	36.0	32.0	36.0
33	34.4853567389384	36.0	36.0	36.0	32.0	36.0
34	34.47587419557221	36.0	36.0	36.0	32.0	36.0
35	34.44702369586702	36.0	36.0	36.0	32.0	36.0
36	34.419937009658376	36.0	36.0	36.0	32.0	36.0
37	34.416505064196855	36.0	36.0	36.0	32.0	36.0
38	33.98474844779796	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	47.0
22	170.0
23	550.0
24	1536.0
25	3835.0
26	8125.0
27	15971.0
28	27479.0
29	43742.0
30	65647.0
31	95699.0
32	138145.0
33	221645.0
34	541644.0
35	1218657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06041174315346	18.210315276919157	12.056663472809616	26.672609507117766
2	16.011423031971482	20.222753320002266	36.51072955069547	27.25509409733078
3	18.2840272692365	23.73493697818419	28.357362127319384	29.623673625259922
4	12.365080590741382	15.859724101348608	35.44347569184491	36.33171961606511
5	14.634934476925396	36.478980872474345	32.836739755449344	16.04934489515091
6	34.98584496692683	35.32039558554332	16.164602933067215	13.529156514462642
7	30.932780952253058	30.196491999650842	20.19958067830069	18.67114636979541
8	28.439222328490548	32.72059548684626	18.91674573841401	19.923436446249188
9	27.392616280777737	14.290690403347309	18.186972524036747	40.12972079183821
10	15.932624330746265	26.36576442436415	31.11187396081545	26.589737284074133
11	37.92670167614928	20.998722921894274	21.894440240676555	19.1801351612799
12	25.30852151813528	23.472856778954355	28.022319051138172	23.19630265177219
13	29.483725929025166	19.109816144271203	25.112667290835834	26.293790635867794
14	23.682238143038916	19.647623236185762	24.684040985285183	31.98609763549014
15	25.292615526007122	27.027808494253865	21.985643573705204	25.69393240603381
16	26.197981035752576	25.709291809786794	23.17478036262461	24.917946791836016
17	24.315111177323477	25.819115743179015	24.82251705376421	25.043256025733296
18	25.323040574424915	24.519315709186895	25.929695057083684	24.227948659304506
19	25.705263113720438	24.830238721224724	25.254552741296866	24.209945423757976
20	25.90275315213993	24.109941437027494	24.77207442176671	25.21523098906587
21	26.94769619435101	24.08014587237007	24.368491400202693	24.603666533076225
22	25.92379966788409	24.161117762661764	24.9487704703045	24.96631209914965
23	24.431260706483105	23.91235560673498	25.521905642783466	26.134478043998456
24	24.879884007125757	24.878289314932825	25.074058764407308	25.16776791353411
25	25.026239003434046	24.298093543883237	25.19028260906969	25.485384843613023
26	24.8141239369593	25.167809879118135	25.48175441320573	24.536311770716836
27	25.59837777566084	24.591160102748706	24.655997038903422	25.15446508268703
28	24.67995996483284	24.47923857644346	25.616086247668285	25.224715211055415
29	24.648485776814436	24.751511285594685	25.508780249317535	25.091222688273344
30	24.73715905585829	24.795743011156553	25.725364628468196	24.741733304516966
31	25.255140259473208	24.650835849519808	24.619655420589577	25.474368470417414
32	24.96373124400679	24.628426227650703	24.67127308893976	25.736569439402746
33	24.568457408079635	24.277887704293708	25.308310654432297	25.845344233194357
34	25.333490004847025	24.43001294638267	25.428667949414685	24.80782909935562
35	25.888275025651463	24.417968823767627	25.23168149800349	24.46207465257742
36	24.721379996265064	25.008130831904758	24.973089569244262	25.29739960258592
37	25.843665609833376	24.617305347884198	24.712902948292108	24.826126093990318
38	24.773416940107875	24.51046014920469	25.345282913557888	25.37083999712955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	137.5
2	175.0
3	175.0
4	591.5
5	1008.0
6	1008.0
7	1312.0
8	1616.0
9	1652.0
10	1688.0
11	1688.0
12	2136.5
13	2585.0
14	3678.5
15	4772.0
16	4772.0
17	7199.0
18	9626.0
19	9626.0
20	11583.0
21	13540.0
22	14726.5
23	15913.0
24	15913.0
25	18724.5
26	21536.0
27	21536.0
28	27874.0
29	34212.0
30	42039.0
31	49866.0
32	49866.0
33	64318.0
34	78770.0
35	78770.0
36	87877.5
37	96985.0
38	111492.0
39	125999.0
40	125999.0
41	135308.0
42	144617.0
43	164015.0
44	183413.0
45	183413.0
46	190077.5
47	196742.0
48	196742.0
49	210239.5
50	223737.0
51	226125.0
52	228513.0
53	228513.0
54	219754.0
55	210995.0
56	210995.0
57	204569.5
58	198144.0
59	177779.5
60	157415.0
61	157415.0
62	147595.0
63	137775.0
64	114658.5
65	91542.0
66	91542.0
67	76681.5
68	61821.0
69	61821.0
70	49605.5
71	37390.0
72	29004.5
73	20619.0
74	20619.0
75	15522.5
76	10426.0
77	10426.0
78	9956.5
79	9487.0
80	7288.5
81	5090.0
82	5090.0
83	4824.5
84	4559.0
85	4559.0
86	2900.0
87	1241.0
88	996.5
89	752.0
90	752.0
91	446.5
92	141.0
93	96.0
94	51.0
95	51.0
96	36.5
97	22.0
98	22.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008644910309055544
2	0.0
3	0.0
4	4.196558402454148E-5
5	1.678623360981659E-4
6	2.9375908817179033E-4
7	3.7769025622087326E-4
8	0.0020143480331779907
9	0.00398673048233144
10	0.0011330707686626198
11	0.00511980125099406
12	3.357246721963318E-4
13	1.678623360981659E-4
14	4.196558402454148E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	8.393116804908296E-5
23	1.2589675207362442E-4
24	0.0
25	8.393116804908296E-5
26	0.0
27	1.678623360981659E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.678623360981659E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2382905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.050774418356916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.64927753210243	39.13004976621394
2	13.901962867781274	14.194119406709518
3	4.232714149442908	6.482500056617921
4	1.7809910857645137	3.63683896641875
5	0.9457232759709404	2.413995281189099
6	0.5385922069924334	1.6497329557593428
7	0.3692934057681197	1.3196900046438518
8	0.25366814752600475	1.035996430117804
9	0.1940929175195562	0.8917734373642457
>10	0.9730746035171496	8.855508940935078
>50	0.070575899912105	2.569243573704228
>100	0.07209935616203704	8.26766768204075
>500	0.011157439898560625	3.931646574641227
>1k	0.0066944639391362595	5.360572941220982
>5k	8.264770295229949E-5	0.26066398242330424
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6178	0.2592633781036172	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4034	0.16928916595500032	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3247	0.13626225132768618	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3086	0.129505792299735	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2720	0.1141463885467528	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2676	0.11229990284967299	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2618	0.10986589897624957	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2580	0.108271206783317	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2440	0.1023960250198812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.196558402454147E-5	0.0
4	0.0	0.0	0.0	4.196558402454147E-5	0.0
5	0.0	0.0	0.0	4.196558402454147E-5	0.0
6	0.0	0.0	0.0	4.196558402454147E-5	0.0
7	0.0	0.0	0.0	4.196558402454147E-5	0.0
8	0.0	0.0	0.0	4.196558402454147E-5	0.0
9	0.0	0.0	0.0	4.196558402454147E-5	0.0
10	0.0	0.0	0.0	8.393116804908294E-5	0.0
11	0.0	0.0	0.0	8.393116804908294E-5	0.0
12	0.0	0.0	0.0	8.393116804908294E-5	2.5179350414724884E-4
13	0.0	0.0	0.0	8.393116804908294E-5	2.5179350414724884E-4
14	0.0	0.0	0.0	8.393116804908294E-5	2.5179350414724884E-4
15	0.0	0.0	0.0	1.2589675207362442E-4	2.9375908817179033E-4
16	0.0	4.196558402454147E-5	0.0	1.6786233609816589E-4	2.9375908817179033E-4
17	0.0	4.196558402454147E-5	0.0	2.0982792012270738E-4	2.9375908817179033E-4
18	0.0	4.196558402454147E-5	0.0	2.5179350414724884E-4	2.9375908817179033E-4
19	0.0	4.196558402454147E-5	0.0	3.3572467219633177E-4	2.9375908817179033E-4
20	0.0	4.196558402454147E-5	0.0	4.1965584024541476E-4	2.9375908817179033E-4
21	0.0	4.196558402454147E-5	0.0	6.294837603681221E-4	2.9375908817179033E-4
22	0.0	4.196558402454147E-5	0.0	8.393116804908295E-4	3.3572467219633177E-4
23	0.0	4.196558402454147E-5	0.0	0.0013848642728098686	3.3572467219633177E-4
24	0.0	4.196558402454147E-5	0.0	0.00255990062549703	3.3572467219633177E-4
25	0.0	4.196558402454147E-5	0.0	0.002937590881717903	3.7769025622087326E-4
26	0.0	4.196558402454147E-5	4.196558402454147E-5	0.0036929713941596496	3.7769025622087326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	590	0.0	18.43994	28
CTAGCGG	615	0.0	17.690348	29
ATCGTTT	505	0.0	16.474588	29
TAGGACG	350	0.0	16.001375	4
CGGTCCA	795	0.0	15.698473	10
TAGCGGC	705	0.0	15.658948	30
ACCGTCG	615	0.0	15.611424	8
CGCTTCG	530	0.0	15.39561	32
TACCGTC	610	0.0	15.214742	7
ATACCGT	600	0.0	15.201625	6
CATCGTT	565	0.0	15.008248	28
TAAACGC	545	0.0	14.971878	28
AGCGGCG	770	0.0	14.752656	31
CGTCGTA	635	0.0	14.61451	10
GTATCAA	4165	0.0	14.599093	1
CGTTTAT	565	0.0	14.441899	31
CCGTCGT	635	0.0	14.363137	9
GTAAACG	575	0.0	14.190737	27
CGCCGGT	760	0.0	14.106771	7
AAACGCT	570	0.0	14.034526	29
>>END_MODULE