FastQCFastQC Report
Thu 2 Feb 2017
SRR4063016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807369
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA52100.6453059257910571No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA45050.5579852582895801No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC41570.5148822905016169No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG37030.4586502578127226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31420.38916530112005787No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA23200.2873531185864209No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA22560.2794261360047265No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA22440.27793982677065876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21270.26344831173849875No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG21220.2628290162243039No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT20600.2551497518482875No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17880.22146007587608643No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA17370.2151432616312987No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA16670.2064731244325705No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT15650.19383949594299507No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA15440.19123845478337662No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT13850.1715448574319797No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13340.16522804318719198No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13280.16448488857015814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12540.15531931496007403No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA11670.14454357301308324No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11310.14008464531088016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.1384744769739735No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG9820.12162963898787295No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG9550.11828544321122064No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA9490.1175422885941868No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT9440.11692299307999193No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT9430.11679913397715294No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG9320.11543668384592423No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA8930.11060617883520422No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT8930.11060617883520422No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA8190.10144060522512012No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA8130.10069745060808627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG308.4224856E-426.6624364
GTACGAC451.2366243E-524.8911043
TCGGTGA451.2384071E-524.88647826
CGGTGAT451.2390017E-524.8849427
GTCTTAC1350.021.3577251
TGCGCAT453.536343E-421.3325910
GTGCGCA453.536343E-421.332599
TAGGACC12600.021.3299484
AAAGGCG453.5393037E-421.3299485
GTCCTAT1450.020.9894891
GACCTAG555.834882E-520.3616647
CACGGTT555.8376645E-520.36040528
CGATTTT801.2647979E-719.99930430
CTGTACG507.138299E-419.2219521
ATTAAGC507.189091E-419.201713
GGCGAGT507.195086E-419.19933119
TGTAGGA40850.018.7722952
CGTTCAA601.1379531E-418.66601814
TCCTATA1650.018.4350192
TTGTAGA7600.018.3367311
Produced by FastQC (version 0.11.5)