##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063016_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	807369
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302118362235856	32.0	32.0	32.0	32.0	32.0
2	31.053930730558147	32.0	32.0	32.0	32.0	32.0
3	31.1163780130275	32.0	32.0	32.0	32.0	32.0
4	31.142509806544467	32.0	32.0	32.0	32.0	32.0
5	31.154608363709777	32.0	32.0	32.0	32.0	32.0
6	34.81956329757521	36.0	36.0	36.0	36.0	36.0
7	34.72743565829255	36.0	36.0	36.0	32.0	36.0
8	34.71285124893326	36.0	36.0	36.0	32.0	36.0
9	34.790192588519005	36.0	36.0	36.0	32.0	36.0
10	34.62122028465299	36.0	36.0	36.0	32.0	36.0
11	34.807496943776634	36.0	36.0	36.0	36.0	36.0
12	34.67368452343352	36.0	36.0	36.0	32.0	36.0
13	34.719466563615896	36.0	36.0	36.0	32.0	36.0
14	34.672686219064644	36.0	36.0	36.0	32.0	36.0
15	34.64059680270112	36.0	36.0	36.0	32.0	36.0
16	34.661450959846114	36.0	36.0	36.0	32.0	36.0
17	34.650974956927996	36.0	36.0	36.0	32.0	36.0
18	34.68849683354203	36.0	36.0	36.0	32.0	36.0
19	34.59390563670391	36.0	36.0	36.0	32.0	36.0
20	34.534541207304216	36.0	36.0	36.0	32.0	36.0
21	34.47978805230322	36.0	36.0	36.0	32.0	36.0
22	34.42949134782237	36.0	36.0	36.0	32.0	36.0
23	34.445761479571296	36.0	36.0	36.0	32.0	36.0
24	34.407284649274374	36.0	36.0	36.0	32.0	36.0
25	34.37833629975885	36.0	36.0	36.0	32.0	36.0
26	34.409372913748236	36.0	36.0	36.0	32.0	36.0
27	34.32937851217969	36.0	36.0	36.0	32.0	36.0
28	34.237644744844054	36.0	36.0	36.0	32.0	36.0
29	34.160271201891575	36.0	36.0	36.0	32.0	36.0
30	34.073717222236674	36.0	36.0	36.0	32.0	36.0
31	34.081600854132375	36.0	36.0	36.0	32.0	36.0
32	34.0394354997529	36.0	36.0	36.0	32.0	36.0
33	34.074616439323286	36.0	36.0	36.0	32.0	36.0
34	34.02032527877588	36.0	36.0	36.0	32.0	36.0
35	34.002558929064655	36.0	36.0	36.0	32.0	36.0
36	33.904633445178106	36.0	36.0	36.0	32.0	36.0
37	33.81676408185105	36.0	36.0	36.0	32.0	36.0
38	33.19797019702268	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	3.0
9	2.0
10	8.0
11	11.0
12	4.0
13	2.0
14	121.0
15	235.0
16	289.0
17	312.0
18	437.0
19	472.0
20	621.0
21	862.0
22	1210.0
23	1813.0
24	2566.0
25	3870.0
26	5859.0
27	8879.0
28	13000.0
29	18505.0
30	25412.0
31	34850.0
32	47238.0
33	72725.0
34	177179.0
35	390881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35174483494134	19.839901783278354	12.606835486991244	25.201517894789056
2	10.761423207049113	20.492619994794957	50.09616933734865	18.649787460807275
3	20.18749450312594	26.046821599029823	30.45980499877985	23.305878899064385
4	9.879705506396219	17.74244691287072	41.36919212437697	31.008655456356088
5	9.400905905478165	41.58792324203679	37.98486194045102	11.026308912034025
6	28.552902765529474	40.687641818848405	19.367004885009067	11.392450530613054
7	25.29227651792427	32.62250594214046	22.64813239051784	19.43708514941743
8	21.001829412544296	45.796500458901384	20.152768952751117	13.048901175803202
9	26.649466723311704	15.018696577888372	20.962338069304295	37.36949862949564
10	14.66316885960838	30.01537102889283	36.981506459919515	18.33995365157928
11	33.71643807959611	20.3320488858006	29.763882128095464	16.187630906507827
12	25.69474377839544	23.69795924434073	35.91747301039948	14.68982396686435
13	31.658920183140744	23.494143063841594	26.33936534992964	18.507571403088022
14	17.894189200906737	27.16483543707961	27.710859790162147	27.230115571851503
15	19.60502043531748	40.532544745230624	23.374435225804373	16.48799959364752
16	17.08261847101014	27.983871467238142	37.29394932271311	17.639560739038608
17	16.97266118601989	31.94830550889242	32.920178789996086	18.158854515091598
18	17.070657148767342	27.42998138287001	40.229598883219936	15.269762585142713
19	23.73595175069707	25.656906232697104	28.087703934191083	22.519438082414744
20	22.708191661061708	30.280768120735125	31.543586751738157	15.467453466465011
21	20.219071318949062	27.496262310839487	27.028540214067085	25.256126156144365
22	21.2470859872217	34.51182079657528	27.93067312766163	16.310420088541395
23	18.34945118378561	33.224408293002575	30.815959211329858	17.610181311881952
24	23.19029090373035	28.461318339087487	32.301921478414805	16.04646927876736
25	20.28418522831446	30.941626817967837	32.5755060578281	16.198681895889596
26	14.695731243511634	33.04984799569879	35.01413503730114	17.240285723488444
27	16.89543475433392	32.5686397165844	32.588706730501244	17.947218798580444
28	16.077391864440372	33.88098895109746	28.680448892632416	21.361170291829758
29	19.03064852183004	29.19871866336671	28.072222593839573	23.698410220963677
30	16.989662322348263	30.510888871823198	31.502047469585232	20.997401336243307
31	22.41889513315059	27.503997800126097	30.37993655543843	19.69717051128488
32	17.667620860806153	33.16805253972127	31.14033838176767	18.023988217704908
33	16.85951088875153	32.69040915346321	34.164828236308395	16.28525172147687
34	17.857717987785996	32.692159996036025	34.01588068428159	15.434241331896391
35	16.881557920295332	33.942494704111596	32.72227246261908	16.453674912973998
36	15.638916864560997	31.936614353192137	34.41750934346156	18.006959438785305
37	16.70095839284321	31.888456302513724	28.75909370745792	22.651491597185146
38	18.402032421130343	35.99558040066245	28.076223224449503	17.526163953757713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	175.0
2	206.0
3	206.0
4	485.0
5	764.0
6	764.0
7	1012.0
8	1260.0
9	1269.0
10	1278.0
11	1278.0
12	1627.5
13	1977.0
14	2657.0
15	3337.0
16	3337.0
17	4474.5
18	5612.0
19	5612.0
20	6899.5
21	8187.0
22	10488.0
23	12789.0
24	12789.0
25	19662.0
26	26535.0
27	26535.0
28	36472.5
29	46410.0
30	61115.5
31	75821.0
32	75821.0
33	89717.0
34	103613.0
35	103613.0
36	106420.5
37	109228.0
38	108970.0
39	108712.0
40	108712.0
41	93695.5
42	78679.0
43	66574.0
44	54469.0
45	54469.0
46	50733.0
47	46997.0
48	46997.0
49	42059.0
50	37121.0
51	31319.0
52	25517.0
53	25517.0
54	22497.0
55	19477.0
56	19477.0
57	16395.0
58	13313.0
59	11203.5
60	9094.0
61	9094.0
62	7711.5
63	6329.0
64	5145.5
65	3962.0
66	3962.0
67	3209.5
68	2457.0
69	2457.0
70	2016.0
71	1575.0
72	1275.0
73	975.0
74	975.0
75	755.0
76	535.0
77	535.0
78	425.0
79	315.0
80	238.5
81	162.0
82	162.0
83	118.0
84	74.0
85	74.0
86	60.5
87	47.0
88	32.5
89	18.0
90	18.0
91	19.5
92	21.0
93	20.0
94	19.0
95	19.0
96	16.5
97	14.0
98	14.0
99	170.0
100	326.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12249665270774578
2	0.056851328203089295
3	0.011395037461185654
4	0.002105604748262566
5	0.0
6	1.238591028389745E-4
7	0.0
8	7.43154617033847E-4
9	2.47718205677949E-4
10	7.43154617033847E-4
11	0.002229463851101541
12	0.0026010411596184644
13	0.014986951443515913
14	0.009784869124278986
15	0.023285511333727204
16	0.012881346695253346
17	0.02043675196843079
18	0.005945236936270776
19	0.007679264376016418
20	0.005945236936270776
21	0.006440673347626673
22	0.007803123478855392
23	0.010651882844151805
24	0.015606246957710785
25	0.0184550063230072
26	0.019198160940041047
27	0.009165573610084113
28	0.0060690960391097505
29	0.010775741946990781
30	0.003839632188008209
31	0.0061929551419487245
32	0.006936109758982572
33	0.008422418993050265
34	0.012262051181058477
35	0.017216415294617454
36	0.01486309234067694
37	0.00990872822711796
38	0.006936109758982572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	807369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.448040875660666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66817981758587	37.32651011598638
2	12.37055103809716	11.739168226201613
3	3.8173598148254517	5.433787335928274
4	1.6044043671160222	3.0450337596803805
5	0.8215814854936135	1.9491215953193493
6	0.5219651946517808	1.4859735534905925
7	0.3301308874940674	1.096484469089565
8	0.24697928938824304	0.9374946734667985
9	0.19266183852118787	0.8227284110399932
>10	1.1517249904412405	10.603971624888334
>50	0.14298136026577762	4.750044453981089
>100	0.1139666867359886	10.706966126310833
>500	0.011501225266847492	3.794301042534023
>1k	0.005750612633423746	5.662243643835135
>5k	2.6139148333744307E-4	0.6461709682477123
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	5210	0.6453059257910571	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	4505	0.5579852582895801	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	4157	0.5148822905016169	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	3703	0.4586502578127226	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3142	0.38916530112005787	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2320	0.2873531185864209	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2256	0.2794261360047265	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2244	0.27793982677065876	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2127	0.26344831173849875	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	2122	0.2628290162243039	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	2060	0.2551497518482875	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1788	0.22146007587608643	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	1737	0.2151432616312987	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1667	0.2064731244325705	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	1565	0.19383949594299507	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1544	0.19123845478337662	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1385	0.1715448574319797	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1334	0.16522804318719198	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1328	0.16448488857015814	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1254	0.15531931496007403	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1167	0.14454357301308324	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1131	0.14008464531088016	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.1384744769739735	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	982	0.12162963898787295	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	955	0.11828544321122064	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	949	0.1175422885941868	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	944	0.11692299307999193	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	943	0.11679913397715294	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	932	0.11543668384592423	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	893	0.11060617883520422	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	893	0.11060617883520422	No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA	819	0.10144060522512012	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	813	0.10069745060808627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.2385910283897449E-4	0.0
8	0.0	0.0	0.0	1.2385910283897449E-4	0.0
9	0.0	0.0	0.0	1.2385910283897449E-4	0.0
10	0.0	0.0	0.0	1.2385910283897449E-4	0.0
11	0.0	0.0	0.0	1.2385910283897449E-4	0.0
12	0.0	0.0	0.0	2.4771820567794897E-4	0.0
13	0.0	0.0	0.0	2.4771820567794897E-4	0.0
14	0.0	0.0	0.0	2.4771820567794897E-4	0.0
15	0.0	0.0	0.0	2.4771820567794897E-4	0.0
16	0.0	0.0	0.0	2.4771820567794897E-4	0.0
17	0.0	0.0	0.0	2.4771820567794897E-4	0.0
18	0.0	0.0	0.0	4.954364113558979E-4	0.0
19	0.0	0.0	0.0	7.43154617033847E-4	0.0
20	0.0	0.0	0.0	7.43154617033847E-4	0.0
21	0.0	0.0	0.0	0.0011147319255507705	0.0
22	0.0	0.0	0.0	0.0013624501312287194	0.0
23	0.0	0.0	0.0	0.0018578865425846174	0.0
24	0.0	0.0	0.0	0.0035919139823302605	0.0
25	0.0	0.0	0.0	0.004335068599364107	0.0
26	0.0	0.0	0.0	0.0054498005249148775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	30	8.4224856E-4	26.662436	4
GTACGAC	45	1.2366243E-5	24.891104	3
TCGGTGA	45	1.2384071E-5	24.886478	26
CGGTGAT	45	1.2390017E-5	24.88494	27
GTCTTAC	135	0.0	21.357725	1
TGCGCAT	45	3.536343E-4	21.33259	10
GTGCGCA	45	3.536343E-4	21.33259	9
TAGGACC	1260	0.0	21.329948	4
AAAGGCG	45	3.5393037E-4	21.329948	5
GTCCTAT	145	0.0	20.989489	1
GACCTAG	55	5.834882E-5	20.361664	7
CACGGTT	55	5.8376645E-5	20.360405	28
CGATTTT	80	1.2647979E-7	19.999304	30
CTGTACG	50	7.138299E-4	19.221952	1
ATTAAGC	50	7.189091E-4	19.20171	3
GGCGAGT	50	7.195086E-4	19.199331	19
TGTAGGA	4085	0.0	18.772295	2
CGTTCAA	60	1.1379531E-4	18.666018	14
TCCTATA	165	0.0	18.435019	2
TTGTAGA	760	0.0	18.336731	1
>>END_MODULE