FastQCFastQC Report
Thu 2 Feb 2017
SRR4063016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807369
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA53980.6685914371247843No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA41200.510299503696575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39370.4876332878770426No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC38420.47586667310734004No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG37630.466081803983061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29970.37120573120840655No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA23670.2931744964198526No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA23050.2854952320438362No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG21930.27162301252587107No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA20900.2588655249334567No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA19430.24065823681612744No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT19300.23904806847922078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19230.23818105475934795No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17390.21539097983697664No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA16580.2053583925070197No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16510.20449137878714688No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14620.18108200835058072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.17426975769443712No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT13880.1719164347404966No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA13780.17067784371210684No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT13590.16832452075816634No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA13320.16498032498151405No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG11340.14045622261939708No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT11010.13636887222571092No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA10230.1267078622042709No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG9930.12299208911910169No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT9290.1150651065374073No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT9170.11357879730333961No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA9090.11258792448062781No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA9090.11258792448062781No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA9040.11196862896643293No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA8990.11134933345223806No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG8980.1112254743493991No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG8940.11073003793804322No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC8820.1092437287039755No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT8780.1087482922926196No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA8570.10614725113300115No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8300.10280305535634883No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT8150.10094516881376421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13400.023.8838774
GGCGTGC350.002064361222.8602838
CGTGCAC350.002065848822.8574510
ACCTAAG350.002065848822.857451
AGTAGGC350.002065848822.8574510
ATAGGAC1950.021.3362643
AGGACCG555.8243717E-520.3664345
GAGTAAT555.829928E-520.3639131
AGGCGTG400.004478780620.0027487
CGTTACC400.004483599719.99903326
TCCGGTG902.0372681E-819.5546112
AGGACCT24100.019.3221575
GGACGTA507.1867515E-419.2026396
TCGACAT507.195743E-419.19907232
CGGTGTT852.395027E-718.82261814
GGACCTG24150.018.752066
TCCGTAA601.1380713E-418.66576426
GGCGATA601.1380713E-418.66576419
TGTAGGA44900.018.4946822
TTAGGCA1055.9590093E-918.2882274
Produced by FastQC (version 0.11.5)