FastQCFastQC Report
Thu 2 Feb 2017
SRR4063015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499111
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41940.2797658078687969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29270.19524905093752232No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15830.10559591651318682No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15800.10539579790956106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15450.10306108086726067No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15300.10206048784913192No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15210.10146013203825466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC1456.91216E-1116.549673
CGTTTAT3650.015.7793931
CTTATAC1850.015.5656363
GTCCTAA3850.015.3952591
TTTCGTT4800.015.33143128
ATCGTTT3550.015.32204229
TATACTG2300.015.3004085
TCCGACT5150.015.22242822
CGACTTT5150.015.22242824
CTATACA2000.015.1960864
CTTTCGT4750.015.15601427
CATCGTT3700.015.13325728
TTCGTTC4900.015.01854529
CCGACTT5250.014.93247723
AATTTCG756.2502216E-414.93197818
GCATCGT3900.014.76739927
CGCTTCG4250.014.68102132
TAGCGTA2950.014.64127357
TCGTTTA3900.014.35767330
TCTAGCG3700.014.26849928
Produced by FastQC (version 0.11.5)