##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063015_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1499111
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23739002648903	32.0	32.0	32.0	32.0	32.0
2	30.815431278937982	32.0	32.0	32.0	32.0	32.0
3	30.875626954908608	32.0	32.0	32.0	32.0	32.0
4	30.949504072747114	32.0	32.0	32.0	32.0	32.0
5	30.846366946810477	32.0	32.0	32.0	32.0	32.0
6	34.519593946012	36.0	36.0	36.0	32.0	36.0
7	34.44720571058447	36.0	36.0	36.0	32.0	36.0
8	34.428451929176696	36.0	36.0	36.0	32.0	36.0
9	34.587840393406495	36.0	36.0	36.0	32.0	36.0
10	34.283003726875464	36.0	36.0	36.0	32.0	36.0
11	34.58078621262869	36.0	36.0	36.0	32.0	36.0
12	34.3756873240207	36.0	36.0	36.0	32.0	36.0
13	34.47255673529178	36.0	36.0	36.0	32.0	36.0
14	34.373413309621505	36.0	36.0	36.0	32.0	36.0
15	34.31059274463332	36.0	36.0	36.0	32.0	36.0
16	34.326662268504464	36.0	36.0	36.0	32.0	36.0
17	34.25540937262151	36.0	36.0	36.0	32.0	36.0
18	34.290049235847114	36.0	36.0	36.0	32.0	36.0
19	34.273523441559696	36.0	36.0	36.0	32.0	36.0
20	34.254688945648454	36.0	36.0	36.0	32.0	36.0
21	34.24264647514427	36.0	36.0	36.0	32.0	36.0
22	34.21604604328832	36.0	36.0	36.0	32.0	36.0
23	34.160813975749626	36.0	36.0	36.0	32.0	36.0
24	34.14692841290605	36.0	36.0	36.0	32.0	36.0
25	34.120104515276054	36.0	36.0	36.0	32.0	36.0
26	34.08200193314571	36.0	36.0	36.0	32.0	36.0
27	34.08149296483049	36.0	36.0	36.0	32.0	36.0
28	34.067304555833424	36.0	36.0	36.0	32.0	36.0
29	34.02223918042093	36.0	36.0	36.0	32.0	36.0
30	34.00070174923671	36.0	36.0	36.0	32.0	36.0
31	34.0192574132269	36.0	36.0	36.0	32.0	36.0
32	33.973587012569446	36.0	36.0	36.0	32.0	36.0
33	33.933938847757105	36.0	36.0	36.0	32.0	36.0
34	33.94685850480718	36.0	36.0	36.0	32.0	36.0
35	33.900157493341055	36.0	36.0	36.0	32.0	36.0
36	33.85281209997125	36.0	36.0	36.0	32.0	36.0
37	33.85260531074751	36.0	36.0	36.0	32.0	36.0
38	33.25490974317445	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	3.0
9	6.0
10	18.0
11	15.0
12	11.0
13	9.0
14	224.0
15	453.0
16	580.0
17	706.0
18	786.0
19	1019.0
20	1319.0
21	1811.0
22	2852.0
23	4327.0
24	6701.0
25	9812.0
26	14502.0
27	20711.0
28	29444.0
29	39425.0
30	52660.0
31	71335.0
32	97424.0
33	147893.0
34	330209.0
35	664851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98258086406395	17.52212791309668	11.607903799348653	25.887387423490722
2	16.229382805700464	20.062619137882244	38.323953588953614	25.384044467463678
3	18.404177391011963	24.592341449811396	29.222123040096708	27.78135811907993
4	12.377097243522735	15.801263599437243	36.79794750145259	35.023691655587434
5	14.210002494820245	36.85436520166019	34.116400041091154	14.81923226242841
6	33.201721559382726	36.300501765719034	17.231069717551662	13.266706957346578
7	29.233325617649392	31.010912467455714	21.70866600271761	18.047095912177284
8	27.449329732960486	33.59626817085405	20.013354643840586	18.94104745234487
9	27.694087213254292	14.088587405702194	18.92253188064628	39.29479350039723
10	15.637572118222856	26.91453077544475	32.32977631260335	25.118120793729044
11	36.87295582548462	21.267553301131294	23.073931492511726	18.785559380872364
12	24.50725853900639	23.922692990683508	29.574227083336112	21.995821386973986
13	29.490898513285956	19.754736215670942	25.541072110034452	25.21329316100865
14	23.361377277667074	20.198711504065827	25.57017979835312	30.869731419913983
15	24.74835226279457	27.720150901901754	22.78394525469033	24.74755158061335
16	25.033240355960345	25.875707420821907	24.613541250397784	24.477510972819964
17	23.506475309754	26.1383667941045	25.930129372752326	24.425028523389177
18	24.52418850207269	24.94539758695359	27.019688889659765	23.510725021313952
19	25.305074117066273	25.103470359844692	25.93717060934769	23.654284913741343
20	25.457703915399826	24.654370502013336	25.59806378101726	24.289861801569575
21	26.580102348163877	24.37703510538018	24.861824641878158	24.18103790457778
22	25.460574944829737	24.79593007586411	25.341762086089624	24.401732893216526
23	24.05041925986907	24.62008431229573	25.9661590005277	25.3633374273075
24	24.570110629712705	25.364707106801504	25.748862145850822	24.31632011763497
25	24.624992077179943	24.795924834955148	25.8155167179843	24.763566369880607
26	24.17729567582751	25.652468930289224	26.281569274763193	23.88866611912007
27	24.782199427204006	25.289016473388603	25.306028234185902	24.622755865221492
28	23.93119458842836	25.194628454780155	26.09215415507568	24.7820228017158
29	24.13583298475111	25.327683966351977	25.84999022635495	24.68649282254196
30	24.05575764529615	25.48298632675565	26.179956892957517	24.281299134990682
31	24.79494758863701	25.167861124064128	25.15071644095058	24.886474846348282
32	24.23311901181726	25.442063143681697	25.166611740940926	25.158206103560122
33	24.001328915179588	24.92089552338377	25.9750881773915	25.102687384045147
34	24.659419632373346	25.095402531969857	26.04515808799983	24.200019747656967
35	25.21701652401276	25.17244899252693	25.878257249396036	23.732277234064274
36	24.17020379461218	25.72284840098688	25.531569951069542	24.575377853331396
37	24.967777487204394	25.488741467371874	25.216818150280996	24.32666289514274
38	24.196903289504405	25.594425654265144	25.698227496814564	24.510443559415883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	506.0
1	416.5
2	327.0
3	327.0
4	821.0
5	1315.0
6	1315.0
7	1489.5
8	1664.0
9	1534.5
10	1405.0
11	1405.0
12	1905.5
13	2406.0
14	3200.0
15	3994.0
16	3994.0
17	5889.0
18	7784.0
19	7784.0
20	9536.5
21	11289.0
22	12361.0
23	13433.0
24	13433.0
25	16110.5
26	18788.0
27	18788.0
28	23690.5
29	28593.0
30	34705.0
31	40817.0
32	40817.0
33	50376.5
34	59936.0
35	59936.0
36	66096.0
37	72256.0
38	80396.5
39	88537.0
40	88537.0
41	92787.0
42	97037.0
43	106764.5
44	116492.0
45	116492.0
46	119763.0
47	123034.0
48	123034.0
49	129001.5
50	134969.0
51	135206.0
52	135443.0
53	135443.0
54	129087.5
55	122732.0
56	122732.0
57	118489.5
58	114247.0
59	101464.0
60	88681.0
61	88681.0
62	83404.5
63	78128.0
64	64207.0
65	50286.0
66	50286.0
67	41887.0
68	33488.0
69	33488.0
70	26894.0
71	20300.0
72	15847.0
73	11394.0
74	11394.0
75	8539.0
76	5684.0
77	5684.0
78	5398.0
79	5112.0
80	4018.0
81	2924.0
82	2924.0
83	2977.5
84	3031.0
85	3031.0
86	1983.5
87	936.0
88	791.0
89	646.0
90	646.0
91	496.0
92	346.0
93	303.0
94	260.0
95	260.0
96	245.0
97	230.0
98	230.0
99	445.5
100	661.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12720872570476768
2	0.056500152423669764
3	0.012340647223587848
4	0.004002372072514977
5	3.335310060429148E-4
6	3.335310060429148E-4
7	0.0
8	4.6694340846008064E-4
9	2.668248048343318E-4
10	6.670620120858296E-4
11	0.0022680108410918207
12	0.003268603859220565
13	0.015675957284016995
14	0.010272754986121775
15	0.02594871227013877
16	0.012007116217544932
17	0.022746814612126787
18	0.006203676712398215
19	0.008738512358324367
20	0.007137563529318375
21	0.007337682132944125
22	0.00767121313898704
23	0.010672992193373273
24	0.01580936968643416
25	0.018410911533568896
26	0.02027868516740922
27	0.009872517778870278
28	0.006737326322066878
29	0.012140528619962098
30	0.003468722462846314
31	0.006937444925692628
32	0.007804625541404205
33	0.009605692974035945
34	0.013274534040508008
35	0.017477024716648734
36	0.016609844100937157
37	0.01060628599216469
38	0.006737326322066878
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1499111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.08876938896531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07667435751202	47.09644239577753
2	11.819833289700936	13.731991403629056
3	3.217137089815636	5.606386035089623
4	1.368723226350202	3.180297914111098
5	0.6737052479460591	1.9567354392037162
6	0.41837207164707507	1.4581631273214368
7	0.270805024676327	1.1011511439457353
8	0.18509818733203398	0.8601700734596816
9	0.13307025518510907	0.6956898629380709
>10	0.6902557274178973	7.268955784622266
>50	0.06330565086747494	2.5851011309585457
>100	0.07231144934604272	8.8645103472818
>500	0.007484381109876539	2.923182303558903
>1k	0.0032240410934852785	2.6712230381025055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4194	0.2797658078687969	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2927	0.19524905093752232	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1583	0.10559591651318682	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1580	0.10539579790956106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1545	0.10306108086726067	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1530	0.10206048784913192	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1521	0.10146013203825466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.670620120858295E-5
2	0.0	0.0	0.0	0.0	6.670620120858295E-5
3	0.0	0.0	0.0	0.0	6.670620120858295E-5
4	0.0	0.0	0.0	0.0	1.334124024171659E-4
5	0.0	0.0	0.0	0.0	1.334124024171659E-4
6	6.670620120858295E-5	0.0	0.0	0.0	1.334124024171659E-4
7	6.670620120858295E-5	0.0	0.0	0.0	1.334124024171659E-4
8	6.670620120858295E-5	0.0	0.0	0.0	1.334124024171659E-4
9	6.670620120858295E-5	0.0	0.0	0.0	1.334124024171659E-4
10	6.670620120858295E-5	0.0	0.0	0.0	1.334124024171659E-4
11	6.670620120858295E-5	0.0	0.0	6.670620120858295E-5	1.334124024171659E-4
12	6.670620120858295E-5	0.0	0.0	6.670620120858295E-5	2.668248048343318E-4
13	6.670620120858295E-5	0.0	0.0	6.670620120858295E-5	3.335310060429148E-4
14	6.670620120858295E-5	0.0	0.0	6.670620120858295E-5	3.335310060429148E-4
15	6.670620120858295E-5	0.0	0.0	1.334124024171659E-4	4.669434084600807E-4
16	6.670620120858295E-5	0.0	0.0	1.334124024171659E-4	4.669434084600807E-4
17	6.670620120858295E-5	0.0	0.0	2.0011860362574886E-4	4.669434084600807E-4
18	6.670620120858295E-5	0.0	0.0	3.335310060429148E-4	5.336496096686636E-4
19	6.670620120858295E-5	0.0	0.0	3.335310060429148E-4	5.336496096686636E-4
20	6.670620120858295E-5	0.0	0.0	4.002372072514977E-4	5.336496096686636E-4
21	6.670620120858295E-5	0.0	0.0	4.669434084600807E-4	6.670620120858296E-4
22	6.670620120858295E-5	0.0	0.0	6.003558108772465E-4	7.337682132944125E-4
23	6.670620120858295E-5	0.0	0.0	7.337682132944125E-4	8.004744145029954E-4
24	6.670620120858295E-5	0.0	0.0	0.0016676550302145738	8.004744145029954E-4
25	6.670620120858295E-5	0.0	0.0	0.002401423243508986	8.004744145029954E-4
26	6.670620120858295E-5	0.0	0.0	0.003068485255594816	8.671806157115783E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	145	6.91216E-11	16.54967	3
CGTTTAT	365	0.0	15.77939	31
CTTATAC	185	0.0	15.565636	3
GTCCTAA	385	0.0	15.395259	1
TTTCGTT	480	0.0	15.331431	28
ATCGTTT	355	0.0	15.322042	29
TATACTG	230	0.0	15.300408	5
TCCGACT	515	0.0	15.222428	22
CGACTTT	515	0.0	15.222428	24
CTATACA	200	0.0	15.196086	4
CTTTCGT	475	0.0	15.156014	27
CATCGTT	370	0.0	15.133257	28
TTCGTTC	490	0.0	15.018545	29
CCGACTT	525	0.0	14.932477	23
AATTTCG	75	6.2502216E-4	14.931978	18
GCATCGT	390	0.0	14.767399	27
CGCTTCG	425	0.0	14.681021	32
TAGCGTA	295	0.0	14.6412735	7
TCGTTTA	390	0.0	14.357673	30
TCTAGCG	370	0.0	14.268499	28
>>END_MODULE